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Application of high-substrate-concentration fermented corn steep liquor product in cultivation and production of edible mushrooms

A fermentation product, corn steep liquor technology, applied in plant cultivation, cultivation, application and other directions, can solve the problems of high bacterial bag contamination rate, rising price, low yield, etc., and achieve the goal of improving nutritional value, reducing raw material cost, and improving yield and quality. Effect

Active Publication Date: 2021-10-01
QIQIHAR UNIVERSITY
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] When looking at the current status of the edible fungus planting industry, the important problems are that the output is low, the pollution rate of the fungus bag is relatively high, and the benefit is relatively low
Black fungus cultivation medium auxiliary nitrogen source raw materials compete with the breeding industry for soybean meal and bran, and the price continues to rise

Method used

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  • Application of high-substrate-concentration fermented corn steep liquor product in cultivation and production of edible mushrooms
  • Application of high-substrate-concentration fermented corn steep liquor product in cultivation and production of edible mushrooms
  • Application of high-substrate-concentration fermented corn steep liquor product in cultivation and production of edible mushrooms

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preparation example Construction

[0052] In the present invention, the preparation method of described aspergillus oryzae seed koji preferably comprises the following steps:

[0053] (1) The making of slant test tube bacterial classification: pass bacterial classification Aspergillus oryzae YY-21 test tube slant medium, constant temperature culture obtains slant test tube bacterial classification;

[0054] (2) The production of triangular bottle seed koji: mix bran and bean cake powder in a weight ratio of 4:1, add sterile water with a weight of 80-90% of the mixed dry material and 10-15% corn steep liquor and mix evenly. Each bottle contains 20g of wet material with a thickness of 1cm. After being sterilized by high pressure, shake well and place it in a sterile room. After the material is cooled, inoculate the spores of the fungus in the inclined test tube, and cultivate it at a constant temperature. When the spores are covered with the koji material, it will be mature. The triangular bottle of koji;

[005...

Embodiment 1

[0067] Adjust the corn steep liquor temperature to 50°C, add 1.7% (w / v) NaOH (i.e. 100mL corn steep liquor plus 1.7g NaOH) and stir for 20min, then add 0.3% (w / v) Ca(OH) 2 (i.e. 100mL of corn steep liquor plus 0.3g of Ca(OH) 2 ) and stirred for 40 minutes, adjusted to maintain the pH at 5.5-6.0, to obtain pretreated corn steep liquor.

[0068] Add 1.5% (w / v) Aspergillus oryzae seed koji (that is, 100mL corn steep liquor plus 1.5g Aspergillus oryzae seed koji) to the pretreated corn steep liquor for fermentation, the temperature is controlled at 50°C, and the reaction time is 180min. Add sodium benzoate, potassium dihydrogen phosphate, magnesium sulfate, ammonium sulfate after reaction, sodium benzoate, potassium dihydrogen phosphate, magnesium sulfate and ammonium sulfate, its consumption ratio is 100mL:0.043g:0.007g:0.007g:0.007g, After the reaction, the temperature was raised to 90°C, the enzyme was inactivated for 15 minutes, and cooled for 30 minutes. After cooling, carr...

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Abstract

The invention provides application of a high-substrate-concentration fermented corn steep liquor product in cultivation and production of edible mushrooms, and belongs to the field of deep processing of agricultural and sideline products. According to the invention, a high-concentration corn steep liquor fermented product is prepared by taking the corn steep liquor as a raw material and adopting a microorganism high-concentration liquid fermentation technology, macromolecular protein in the corn steep liquor can be moderately degraded, so that the corn steep liquor has reasonable molecular weight distribution and has the characteristics of quick-acting and medium-long-acting nitrogen source nutrition supply; nutrient substances in the high-concentration corn steep liquor fermented product can activate synthesis and secretion of enzyme systems of the edible mushrooms and accelerate decomposition of cellulose, lignin and the like in a mushroom bag matrix, so that edible mushroom mycelia can quickly absorb nutrient substances which cannot be utilized in a normal state in the initial vegetative growth stage, generation of various induction enzymes is accelerated, the mycelia are robust and rapidly enter stages of culture medium material decomposition, absorption and resynthesis, and the beneficial effects of high mycelium growing speed, tidy fruiting, improvement of the yield and quality of sporocarp and the like are achieved.

Description

technical field [0001] The invention relates to the technical field of deep processing of agricultural and sideline products, in particular to the technical method of fermenting corn steep liquor with high substrate concentration and the application of the product in the cultivation and production of edible fungi. Background technique [0002] Corn steep liquor is a by-product of cornstarch produced by wet process. It has the characteristics of high yield, low price and rich nutrition. It is a good quality nutrient nitrogen source. However, untreated corn steep liquor has high concentration, many impurities, high content of anti-nutritional factors, high acidity, high viscosity and heterogeneity in the system, resulting in the failure to use it rationally and resulting in the waste of high-quality resources. Therefore, the deep processing and utilization of corn steep liquor is imminent. [0003] The in-situ fermentation of corn steep liquor by using microbial high-concentr...

Claims

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Application Information

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IPC IPC(8): A01G18/22A01G18/40A01G18/30
CPCA01G18/22A01G18/40A01G18/30Y02P60/87
Inventor 刘晓兰郑喜群于梦尧钱朋智刘井权李冠龙王晓燕
Owner QIQIHAR UNIVERSITY
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