Brevibacillus brevis microecological preparation
A technology of Bacillus brevis and micro-ecological preparations, applied in the field of micro-ecological preparations, can solve problems such as susceptible to contamination of miscellaneous bacteria, and achieve the effects of inhibiting the growth of miscellaneous bacteria, solving susceptible to contamination and miscellaneous bacteria, and improving the effect of weight gain.
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Embodiment 1
[0018] The preparation of embodiment 1 bacillus brevis probiotics
[0019] Streak Bacillus brevis FJAT-0809-GLX (Brevibacillus brevis) on the strain medium with an inoculation loop, and cultivate it in a constant temperature incubator for 24 hours, and the cultivation temperature is 30°C to obtain a single colony; inoculate the obtained single colony Put it in 100ml of seed culture medium, and place it in a constant temperature shaking shaker for 24 hours, the temperature is 30°C, and the rotation speed is 180rpm / min. After the cultivation, the seed liquid is obtained; the seed liquid is put into the fermenter according to the inoculum amount of 1%. Liquid fermentation is carried out in the fermenter, the fermenter is equipped with sterilized fermentation medium, the fermentation parameters are temperature 30 ℃, rotating speed 170rpm / min, air flow 30L / min, fermentation time 48h, then obtain liquid fermented liquid, standby; Take The liquid fermented liquid that obtains, adds N...
Embodiment 2
[0022] The antibacterial activity test of embodiment 2 Bacillus brevis probiotics
[0023] Place the Bacillus brevis probiotics prepared in Example 1 at room temperature in a cool and dry place, and take samples when storing 0d, 10d, 20d, 30d, and 50d respectively; A test tube filled with 9mL of sterile water will make 10 -1 Dilution of the dilution factor, serially diluted to 10 -5 Dilution of the dilution factor) and spread on the NA plate (the components of the NA plate: beef extract 0.3%, peptone 1.0%, sodium chloride 0.5%, agar powder 1.7%, distilled water preparation, pH7.0-7.2), After culturing at 30°C for 48 hours, the number of viable bacteria in each sample was counted, and the antibacterial activity of each sample was detected by the agar diffusion method.
[0024] Test result shows, the Bacillus brevus probiotics prepared in embodiment 1 prolongs along with storage time, viable count and bacteriostatic effect are all more stable, and the viable count of 0d and 30...
Embodiment 3
[0025] Example 3 Effect of Bacillus brevis probiotics on production performance of weaned piglets
[0026] In order to verify the impact of the Bacillus brevis probiotics of the present invention on feeding animals, the applicant conducted the following tests.
[0027] Test location: pig farm in Fuqing National Modern Agriculture Demonstration Park;
[0028] Experimental animals: piglets with a weaning age of 30 days;
[0029]Experimental grouping: 150 weaned piglets of about 5kg were randomly divided into 5 groups (1 as the control group, 4 as the test group, namely the treatment group), each group had 3 columns (repeated), and each replicated 10 pigs, carried out test.
[0030] The conventional ration fed on the pig farm was used as the basal ration, and its composition ratio was shown in Table 1; the feeding scheme of the control group and the test group was shown in Table 2; And the deworming program is carried out according to the pig farm feeding management program; C...
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