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Construction method of SD rat myocardial ischemia model

A technique of myocardial ischemia and construction method, applied in the field of medical experiments, can solve the problems of cumbersome operation of cutting the ribs of rats, low success rate of tracheal intubation, failure of model construction, etc., so as to increase the success rate of experiments and reduce the death of pneumothorax. Probability, easy to operate effect

Active Publication Date: 2021-09-24
湖南丰晖生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 1. The incision in the trachea during tracheal intubation caused a certain degree of damage to the rats, and the rats may die later due to bleeding and improper suturing. The model construction failed, that is, the success rate of tracheal intubation was low
[0008] 2. Cutting the fourth rib of the rat in the process of thoracotomy has the risk of massive bleeding, and this process has caused great damage to the rat, which affects the survival of the subsequent rat; the operation of cutting the rib of the rat is cumbersome and prolongs the operation time
[0009] 3. In the process of chest cavity suturing, there is a certain probability that insufficient exhaust will occur by directly squeezing the chest cavity, resulting in pneumothorax and death of the rat; during the suture process of this step, the suture needle may puncture the lung, resulting in large rat death phenomenon

Method used

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  • Construction method of SD rat myocardial ischemia model
  • Construction method of SD rat myocardial ischemia model
  • Construction method of SD rat myocardial ischemia model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A construction method of SD rat myocardial ischemia model, comprising the following steps:

[0044] S1. Select healthy SPF rats with a body weight of 300g-350g, and remove the hair from the chest cavity and neck trachea after anesthesia.

[0045] S2. Put the rat in the supine position, and fix the limbs.

[0046] S3. Set the relevant parameters of the ventilator, that is, the respiratory ratio is 1.25:1, the tidal volume is 2ml / 100g, and the respiratory rate is 70 times / min, and then connect the ventilator catheter to the tracheal intubation needle.

[0047] S4. Use ophthalmic scissors to cut the rat neck skin, separate the muscle layer layer by layer, expose the trachea, and then insert the tracheal intubation needle into the rat trachea to connect the ventilator to the rat trachea.

[0048] S5. Use ophthalmic scissors to cut open the left chest skin of the rat with an opening size of about 3 cm, and separate the superficial and deep pectoralis muscles layer by layer....

Embodiment 2

[0058] A construction method of SD rat myocardial ischemia model, comprising the following steps:

[0059] S1. Select healthy SPF rats with a body weight of 300g-350g, and remove the hair from the chest cavity and neck trachea after anesthesia.

[0060] S2. Put the rat in the supine position, and fix the limbs.

[0061] S3. Set the relevant parameters of the ventilator, that is, the respiratory ratio is 1.25:1, the tidal volume is 2ml / 100g, and the respiratory rate is 70 times / min, and then connect the ventilator catheter to the tracheal intubation needle.

[0062] S4. Use ophthalmic scissors to cut open the rat neck skin, separate the muscle layer layer by layer, and expose the trachea.

[0063] S5. Hold the tweezers in the left hand to carefully pull the rat’s tongue out of the mouth, and hold the tracheal intubation needle in the right hand to insert the tracheal intubation needle from the rat’s mouth close to the palate. When the naked eyes see the indication on the needl...

Embodiment 3

[0069] Such as figure 1 , figure 2 with image 3 As shown, the endotracheal intubation needle of the present invention includes a needle part 1 and a needle tail part 2 . The rear end of the needle part 1 is provided with a male buckle 4 (also called a tang), and the front end of the needle tail part 2 is provided with a female buckle 5 (also called a socket), and the male buckle 4 and the female buckle 5 are snap-connected. The needle part 1 can be detached from the tracheal intubation needle, that is, the needle part 1 can be separated from the needle tail part 2, and the operator can choose the needle part 1 of the required size according to the weight of the experimental rats to meet the needs of rats with different weights. pipe size requirements. In the actual application process, the positions of the male button 4 and the female button 5 of this embodiment can be interchanged, that is, the rear end of the needle part is set as a female button, and the front end of t...

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Abstract

The invention discloses a construction method of an SD rat myocardial ischemia model, and relates to the technical field of medical tests. The construction method of the SD rat myocardial ischemia model comprises the following steps: anesthetizing a rat; cutting the neck skin of the rat open, separating neck muscles until exposing the trachea, and inserting a trachea cannula needle into the trachea of the rat to be connected with a breathing machine; conducting unhairing and skin preparation on the left chest of the rat, separating shallow chest muscles and deep chest muscles, forming an incision between a third rib and a fourth rib, pre-burying a suture line in the muscles between the third rib and the fourth rib, and ligating the left anterior descending branch of the heart through the suture line; suturing the thoracic cavity of the rat by using the suture line, and exhausting the thoracic cavity of the rat by using an injector connected with a soft silica gel catheter; and pulling out the tracheal intubation needle, and suturing layer by layer. According to the construction method of the SD rat myocardial ischemia model, the success rate of rat myocardial ischemia model construction is increased, the operation difficulty of model construction is reduced, and the operation time of model construction is shortened.

Description

technical field [0001] The invention belongs to the technical field of medical experiments, and in particular relates to a method for constructing an SD rat myocardial ischemia model. Background technique [0002] Biology takes animals, plants, microorganisms as the research objects, and expounds the law of biological development by setting up experiments under various conditions. The development of biology has gone through a history of nearly a hundred years, gradually moving from theoretical biology to Experimental Biology. With the development of biology, a new discipline—experimental zoology has gradually formed, that is, a discipline that uses experimental animals as research objects to conduct animal experiments. A variety of experimental objects are used in teaching, scientific research, production, etc. Various model organisms such as zebrafish, mice, Arabidopsis, and Escherichia coli have been developed. Through the research on these experimental objects, people re...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61D7/00A61D3/00
CPCA61D1/00A61D7/00A61D3/00A61D2003/006Y02A50/30
Inventor 李浩仇庆刘彩云周婧雯徐彬
Owner 湖南丰晖生物科技有限公司
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