Shape memory composite material with both electrical response and temperature response as well as preparation method and application of shape memory composite material in immobilized cells

A composite material, temperature-responsive technology, applied in the direction of immobilization on or in inorganic carriers, immobilized enzymes, microorganism-based methods, etc. The effect of fast response rate and simple preparation process

Active Publication Date: 2021-08-24
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on media infiltration regulation has made outstanding progress, but it has not been well applied to the adhesion research of immobilized cells and biofilms, and the stimulus response mode is relatively single, which limits its application in biological media to a certain extent. field application

Method used

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  • Shape memory composite material with both electrical response and temperature response as well as preparation method and application of shape memory composite material in immobilized cells
  • Shape memory composite material with both electrical response and temperature response as well as preparation method and application of shape memory composite material in immobilized cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Embodiment 1: Photoetching of silicon wafers and shape memory composite materials are prepared according to the following method:

[0088] (1) After the clean silicon wafer is soaked in a mixed solution of 98% concentrated sulfuric acid and 30% hydrogen peroxide (7:4, v / v), the surface is ablated point by point with a picosecond pulse laser, and the wavelength of the picosecond pulse laser is set The frequency is 1064nm, the frequency is 0.4MHz, the scanning speed is 190μm / s, the pulse width is 12ps, and the surface micropattern with a length of 10μm, a width of 12μm, a height of 30μm, and an array spacing of 650nm is obtained, and the photoetched silicon piece.

[0089] (2) Take a certain amount of N,N-dimethylformamide (DMF), add two-dimensional graphene to make a 1mg / mL suspension, and disperse evenly by ultrasonication for 1h; into a 50mg / mL solution, magnetically stirred until completely dissolved; then the two-dimensional graphene dispersion and thermoplastic pol...

Embodiment 2

[0091] Example 2: Grafting poly-N-isopropylacrylamide on the surface of the shape memory composite material is prepared according to the following method:

[0092] The reversible shape memory composite material with micropatterns obtained in Example 1 was treated with aminopropyltrimethoxysilane to modify amino groups. Specifically, include the following steps:

[0093] (1) Adjust the mass fraction of the mixed solution of aminopropyltrimethoxysilane and anhydrous toluene to 7wt%, and immerse the reversible shape memory composite material with micropatterns obtained in Example 1 in the mixed solution, at 110°C , under the protection of nitrogen atmosphere, toluene was refluxed for 18 hours, and after the reaction was completed, it was blown dry with nitrogen to obtain a shape memory composite material with surface-modified amino groups;

[0094](2) Subsequently, using a dry dichloromethane solution containing 2% (v / v) pyridine as a solvent, the shape memory composite material...

Embodiment 3

[0096] Example 3: Analysis of surface wettability switching of thermoplastic polyurethane composite materials with both electrical and temperature responses:

[0097] Detection method: refer to the literature Chemical Engineering Journal, 382(2020) 122989.

[0098] Such as figure 1 As shown, when the columnar array on the surface of the composite material remains in an upright state, the surface contact angle of the composite material is 165±2° at a current of 80mA (temperature T≈37°C), showing superhydrophobicity. ≈25℃), the surface contact angle of the composite material is close to 0°, showing superhydrophilicity.

[0099] When the columnar array structure collapses due to external force, the surface contact angle of the composite material is 115±2° under the condition of current of 80mA (T≈37℃), showing hydrophobicity. Under the conditions, the surface contact angle of the composite material is 40±1°, showing hydrophilicity.

[0100] When the columnar array structure co...

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Abstract

The invention discloses a preparation method of a shape memory composite material with both electrical response and temperature response, wherein the preparation method comprises the following steps: (1) mixing thermoplastic polyurethane and two-dimensional graphene, and curing to obtain the shape memory composite material with electrical response; (2) carrying out hot stamping on the shape memory composite material obtained in the step (1) by taking a silicon wafer subjected to photoetching as a template to obtain a shape memory composite material with a micro-pattern; and (3) grafting a temperature-responsive monomer to the shape memory composite material with the micro-pattern obtained in the step (2) to obtain the shape memory composite material with both electrical response and temperature response. According to the shape memory composite material with electrical response and temperature response simultaneously provided by the invention, by virtue of excellent electrical and thermal conductivity and electrical transmission characteristics of two-dimensional graphene, under the condition that normal growth and metabolism of cells are not influenced, the surface wettability of a temperature regulation and control material can be changed through impressed current; and switching of the surface wettability of the composite material can be realized without the help of external force.

Description

technical field [0001] The invention relates to the field of polymer material immobilized cells, in particular to a shape memory composite material with both electrical and temperature responses, a preparation method thereof and an application in immobilized cells. Background technique [0002] In the process of immobilizing cells, microorganisms adsorbed and aggregated on the solid surface will synthesize a large amount of extracellular polymeric matrix (EPS) such as polysaccharides and proteins, thereby embedding their own cells in it, forming a colony shape with a specific three-dimensional structure—biofilm ( biofilm) to achieve long-term colonization of cell populations on the surface of solid media. At the same time, EPS provides a natural barrier for cells to isolate external toxic substances, thus improving the stress resistance of microbial cells in EPS. [0003] Hydrophobic interaction is one of the key factors affecting cell adhesion to the carrier surface. "Foo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/16C08L75/04C08K3/04C12N11/093C12N11/14C12N13/00C12P13/08C12R1/15
CPCC08J7/16C08K3/042C12N11/093C12N11/14C12N13/00C12P13/08C08J2375/04C08K2201/001C08L75/04
Inventor 应汉杰沙宇庄伟朱晨杰李明欧阳平凯陈勇柳东沈涛谭卓涛
Owner NANJING TECH UNIV
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