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SPR sensor chip and preparation method thereof

A sensor chip, gold chip technology, applied in the field of biomedical detection, can solve the problems of fast response, difficult to obtain sensitivity, easy aggregation, etc.

Active Publication Date: 2021-08-17
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the thickness of the sensitive layer of the SPR biosensor is ~200nm, and the MXene nanosheets tend to aggregate due to the π-π* stacking effect, forming large-sized aggregates.
Limited by the thickness of SPR biosensors, it is difficult to obtain SPR sensor chips with high sensitivity and fast response using MXene nanosheets.

Method used

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  • SPR sensor chip and preparation method thereof
  • SPR sensor chip and preparation method thereof
  • SPR sensor chip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0051] The preparation of SPR sensor chip in embodiment 2 and the test of SPR sensor chip in experiment example 3 and experiment example 4 are all by Biacore TM X100 instrument (GE-Healthcare Bio-Sciences AB, USA) was performed at 25°C.

Embodiment 1

[0054] The SPR sensor chip of the present embodiment comprises a glass substrate and a gold layer disposed on the glass substrate, and the surface of the gold layer has thiol-functionalized Nb modified by Au-S covalent bonds. 2 C MXene quantum dots, the N-58 aptamer is anchored on the thiol-functionalized MXene quantum dots.

[0055] Among them, sulfhydryl functionalized Nb 2 The preparation method of C quantum dots, comprises the following steps:

[0056] (1) Preparation of Nb 2 C MXene powder: Nb 2 AlC powder was reacted with 3% hydrofluoric acid at 55°C for 48h, then rinsed with deionized water for 12 times, and dried in a vacuum oven at 60°C for 18h to obtain Nb 2 C MXene powder.

[0057] (2) Preparation of Nb 2 C MXene quantum dots: 15mg Nb 2 C MXene powder was dispersed in 20mL ultrapure water (≥18.2MΩ·cm), stirred at a stirring speed of 800 rpm for 0.5h, then added ammonia water with a mass fraction of 25%, and adjusted the pH of the system to 6 , and then heated...

experiment example 1

[0068] Experimental Example 1 Structural Characterization

[0069] 1. Infrared spectroscopy

[0070] Utilize infrared spectrum to the Nb prepared in embodiment 1 2 C MXene quantum dots and Nb 2 C-SH quantum dots were characterized, and the obtained results were as follows image 3 shown.

[0071] From image 3 It can be seen that Nb 2 C MXene quantum dots and Nb 2 C-SH quantum dots are decorated with a sufficient number of oxygen-containing functional groups. 3496, 3131 and 3034cm -1 The bands at are assigned to Nb 2 O-H stretching, N-H stretching and C-H stretching of C quantum dots, indicating the presence of hydroxyl and amino groups. C=O stretching vibrations due to carbonyl and carboxyl groups, 1741 and 1634 cm -1 There are two typical strong bands. In addition, 1400cm -1 The band at the place belongs to the C-O-C characteristic band, 450~950cm -1 The broad band of is the typical vibration peak of Nb-O. for Nb 2 C-SH quantum dots, 3296cm -1 The nearby broa...

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Abstract

The invention relates to an SPR sensor chip and a preparation method thereof, and belongs to the technical field of biomedical detection. The SPR sensor chip comprises a glass substrate and a gold layer arranged on the glass substrate, the surface of the gold layer is provided with sulfydryl functionalized MXene quantum dots modified by Au-S covalent bonds, and aptamers are anchored on the sulfydryl functionalized MXene quantum dots. According to the preparation method, the sulfydryl-functionalized MXene quantum dots are uniformly coated on the surface of the SPR gold chip through a self-assembly effect between the sulfydryl-functionalized MXene quantum dots and the SPR gold chip, and then an aptamer is fixed on the MXene quantum dots through pi-pi * accumulation, electrostatic adsorption and hydrogen-bond interaction. Compared with a previously reported SPR sensor chip, the SPR sensor chip based on the sulfydryl functionalized MXene quantum dots has the advantages of simple structure and high stability, and shows excellent sensing properties such as relatively high sensitivity, quick response and practicability in a complex environment.

Description

technical field [0001] The invention relates to an SPR sensor chip and a preparation method thereof, belonging to the technical field of biomedical detection. Background technique [0002] Since the end of 2019, the global epidemic of COVID-19 caused by a novel coronavirus (SARS-CoV-2) has continued to spread. The early clinical manifestations of COVID-19 are similar to those of severe acute respiratory syndrome and MERS-CoV, namely fever, headache, myalgia, arthralgia, and swollen lymph nodes. SARS-CoV-2 has the characteristics of moderate mortality, high infection rate, and long incubation period, which can lead to long-term infection. The novel coronavirus (SARS-CoV-2) has been found in multiple environments such as water systems, frozen foods, and food packaging. Therefore, rapid diagnosis of the COVID-19 virus is crucial for effective control of virus transmission and treatment of patients. Similar to other coronaviruses, the new coronavirus (SARS-CoV-2) is mainly co...

Claims

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Application Information

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IPC IPC(8): G01N21/552
CPCG01N21/554Y02A50/30
Inventor 陈荣源崔静何领好张治红王明花薛雨林
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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