Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Antibody diluent for Rh system typing detection card and detection card

A technology of antibody diluent and detection card, which is applied in the direction of measuring devices, biological testing, and analytical materials, etc. It can solve the problems of destroying the ionic strength of the reaction system, difficulty in ensuring sensitivity, and inability to balance sensitivity and specificity, so as to improve stability and sensitivity, avoiding missed detection, and preventing titer decline

Active Publication Date: 2021-08-13
TIANJIN DEXIANG BIOTECHNOLOGY CO LTD
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, some manufacturers at home and abroad have produced Rh blood type test cards, but there are some shortcomings: one is the use of a single type of dextran gel, which makes it difficult to balance sensitivity and specificity; gel, so that the gel cannot fully swell completely, and destroy the ionic strength of the reaction system; the third is that for gels with different antibodies added, the gel washing buffer is completely consistent, which cannot effectively protect the stability of the antibody and take into account the sensitivity of the test results
However, in further research, it was found that the antibody diluent was suitable for the preparation of glass microbead blood type detection card, and when preparing the Rh system typing detection card, due to the anti-D gel, anti-C gel, anti-c gel, anti-c gel, The properties of the six microcolumns of anti-E gel, anti-E gel and blank gel are difficult to unify, making it difficult to guarantee the sensitivity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antibody diluent for Rh system typing detection card and detection card
  • Antibody diluent for Rh system typing detection card and detection card
  • Antibody diluent for Rh system typing detection card and detection card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Antibody diluent components: potassium dihydrogen phosphate 0.24g / L, disodium hydrogen phosphate 1.44g / L, potassium chloride 0.2g / L, sodium chloride 8g / L, bovine serum albumin 30g / L, mannitol 1.5 g / L, trehalose 5g / L and polysucrose 8g / L.

[0036] Preparation:

[0037] 1) Dextran gel treatment: Take an appropriate amount of Dextran gel, rinse with normal saline for 3 times, every 2h interval, for use;

[0038] 2) Preparation of antibody diluent: fully mix the components of the above antibody diluent to obtain the antibody diluent, which is for later use;

[0039] 3) Preparation of antibody working solution:

[0040] Take the anti-C monoclonal antibody concentrate and mix it with the antibody diluent, so that the titer of the obtained anti-C monoclonal antibody working solution is greater than or equal to 64;

[0041] Take the anti-c monoclonal antibody concentrate and mix it with the antibody diluent, so that the titer of the obtained anti-c monoclonal antibody workin...

Embodiment 2

[0051] Antibody diluent components: potassium dihydrogen phosphate 0.1g / L, disodium hydrogen phosphate 0.5g / L, potassium chloride 0.1g / L, sodium chloride 5g / L, bovine serum albumin 10g / L, mannitol 1g / L, trehalose 3g / L and polysucrose 5g / L.

[0052] Preparation method: same as Example 1.

Embodiment 3

[0054] Antibody diluent components: potassium dihydrogen phosphate 1g / L, disodium hydrogen phosphate 1.5g / L, potassium chloride 1g / L, sodium chloride 15g / L, bovine serum albumin 20g / L, mannitol 2g / L , trehalose 8g / L and polysucrose 12g / L.

[0055] Preparation method: same as Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of blood typing testing, and particularly relates to a composition of an antibody diluent for an Rh system typing detection card and the antibody diluent. The composition is composed of mannitol, trehalose and polysucrose in a mass ratio of (1-2) : (3-8) : (5-12). When the composition is used for preparing the antibody diluent, only one antibody diluent needs to be prepared, the composition can be suitable for various related antibodies of the Rh system typing detection card, different antibody diluents do not need to be prepared for different antibodies, and the preparation process of the Rh system typing detection card is simplified; meanwhile, the prepared antibody diluent can remarkably improve the stability of the antibody, prevent the titer of the antibody from being reduced, and avoid the phenomenon of leak detection. Moreover, the antibody diluent is suitable for a microcolumn gel immunodetection technology, sensitivity and specificity can be considered by adopting sephadex of a single model, and the stability of the antibody is effectively protected.

Description

technical field [0001] The invention belongs to the field of blood typing test, and in particular relates to an antibody dilution liquid composition and antibody dilution liquid for Rh system typing detection card. Background technique [0002] The Rh system is an extremely complex blood group system, second only to the ABO system in importance. The D antigen is the most important antigen in the Rh blood group antigen system. The presence of D antigen on the membrane of human red blood cells is RhD positive, and the absence of D antigen is RhD negative. In addition to D antigens, C, c, E, and e antigens are also the main antigens in the Rh system, which have strong clinical significance. In addition, since most of the irregular antibodies belong to the Rh blood group system, after the blood containing the corresponding antigen is transfused, it can cause a severe immune hemolytic transfusion reaction, and the IgG nature of the Rh system antibody has a strong ability to pass...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80G01N33/577G01N33/531G01N33/53
CPCG01N33/80G01N33/577G01N33/531G01N33/5306
Inventor 商巧娜王丽王伟权王秀柱黄志刚
Owner TIANJIN DEXIANG BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com