Porous scaffold for guiding oral soft and hard tissue integrated repair and preparation method thereof

Abstract

The invention provides preparation and application of a porous scaffold for guiding oral soft and hard tissue integrated repair. The integrated stent is prepared by combining electrostatic spinning with 3D printing. The composition and aperture of the integrated stent are in gradient change, and the upper layer is a b-FGF-loaded heparin grafted polycaprolactone / gelatin microporous fiber membrane, plays a barrier role and is used for guiding soft tissue regeneration; and the lower layer is a polycaprolactone / gelatin / hydroxyapatite macroporous scaffold and is used for guiding hard tissue regeneration. The interface of the upper-layer fiber membrane and the interface of the lower-layer 3D stent are firmly combined, the 3D stent has a through porous structure, and the compressive strength of the 3D stent is 13.86 MPa and is similar to that of cancellous bones of a human body. Both the upper-layer fibrous membrane and the lower-layer scaffold have good cell compatibility, and the lower-layer scaffold can effectively promote osteogenic differentiation of the BMSCs. The porous scaffold capable of effectively guiding in-vivo soft and hard tissue integrated repair is prepared and can be applied to synchronous regeneration of periodontal tissue and alveolar bone defects.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a porous scaffold for guiding the integrated repair of soft and hard tissues in the oral cavity and a preparation method thereof. Background technique [0002] Periodontitis is the most common oral disease, and severe cases can lead to alveolar bone defects, periodontal attachment loss, tooth loss and other symptoms of periodontal bone defects. Guided tissue regeneration (GTR) is based on the different migration speeds of various tissue cells, using surgical means to place a biofilm with a barrier function between the soft tissue and the bone defect area to form an isolation space to prevent interference. Bone repairing and fast migrating epithelial cells and connective tissue cells enter the bone defect area, avoiding the competitive inhibition of these cells on the precursor osteoblasts with potential bone formation ability and slow migration speed, and realizing the reparab...

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Problems solved by technology

[0004] The purpose of the present invention is: the present invention provides an integrated porous composite scaffold and its preparation method, which solves the relatively large alveolar bone defect in the prior art, and the implanted bone powder is easy to shift, and any small movement will cause damage to the bone. The effect of regeneration has a certain impact on technical issues

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