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Method for preparing taurine from mussels

A technology of taurine and mussel, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of low extraction rate and high extraction cost

Pending Publication Date: 2021-08-03
ZHEJIANG ECONOMIC & TRADE POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the invention is to solve the problems that the existing natural taurine extraction method has high extraction cost and low extraction rate

Method used

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  • Method for preparing taurine from mussels
  • Method for preparing taurine from mussels
  • Method for preparing taurine from mussels

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Freeze-drying: Take an appropriate amount of fresh mussel meat, weigh it, freeze it in a -20°C refrigerator for 4 hours, take it out, put it into a pre-cooled freeze dryer, dry it for 48 hours, and take it out. Freeze dryer model: FD-1A-50. Freeze-drying conditions: below -50°C, vacuum below 1000Pa.

[0039] (2) Grinding: crush the freeze-dried mussel meat with a small universal grinder, pass through an 80-mesh sieve to obtain freeze-dried mussel powder, weigh it, and calculate that the dry weight ratio is about 1:4, that is, 1g mussels The powder is equivalent to the quality of 4g mussel fresh meat, and the mussel powder is stored in a refrigerator at 4°C.

[0040] (3) Extraction: Take 25g of freeze-dried mussel powder, which is equivalent to about 100g of fresh mussel meat, add purified water at a ratio of 1:20, put it in a constant temperature water bath at 60°C, heat and extract for 2 hours, and cool to At room temperature, filter with 4 layers of gauze, and t...

Embodiment 2

[0047] (1) Homogenization: Weigh 100g of fresh mussel meat and put it into a tissue homogenizer for homogenization

[0048](2) Extraction: Add purified water to the homogenate at a material-to-liquid ratio of 1:5, place it in a constant temperature water bath at 60°C, heat and extract for 2 hours, cool to room temperature, filter with 4 layers of gauze, and concentrate the filtrate to its About 1 / 6 times the volume.

[0049] Subsequent steps such as protein removal, purification, determination of extraction amount, concentration, and recrystallization are consistent with steps (3) to (8) in Example 1, and the resulting sample solution has a maximum absorption wavelength at a wavelength of 400nm, which is similar to that of taurine. The standard is the same. After the standard curve of taurine was obtained by measuring taurine standard substance by spectrophotometry, the extraction amount of taurine in fresh mussel meat was determined to be 0.5988 mg / g. After recrystallizatio...

Embodiment 3

[0051] Same as the operation steps of Example 1, under the conditions of 1:5, 1:20, 1:100, and 1:150 respectively at the ratio of solid to liquid, the extraction rate of taurine obtained is respectively 0.7243mg / g, 0.9030mg / g, 0.8876mg / g, 0.7901mg / g. Therefore, the suitable ratio of solid to liquid in this experiment is 1:20.

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Abstract

The invention relates to a method for preparing taurine from mussels, in particular to a method for extracting taurine from freeze-dried mussel powder. The method comprises the following steps: freeze-drying fresh mussels, grinding the freeze-dried mussels to prepare freeze-dried mussel powder, extracting the freeze-dried mussel powder with water, conducting precipitating to remove protein, carrying out purifying through a cation exchange column, concentrating eluate, adding absolute ethyl alcohol to separate out a crude taurine product, and adding absolute ethyl alcohol for recrystallization to obtain a pure taurine product. The freeze-dried mussel powder is prepared from fresh mussel meat by using a cell disruption technology and then is extracted, so the extraction rate of the natural taurine in the mussels can be increased. Moreover, technical support is provided for realizing comprehensive utilization of mussel resources and promoting healthy and sustainable development of the mussel industry.

Description

technical field [0001] The invention relates to a method for preparing taurine, in particular to a method for preparing taurine from mussels. Background technique [0002] Taurine, also known as oxcholine and oxcholine, is a non-protein amino acid. Taurine has anti-inflammatory, antipyretic and analgesic effects, promotes brain development, enhances vision, regulates nerve conduction and promotes absorption. The normal performance of the function has an important regulatory role, so it is widely used in many fields such as food industry, feed industry and pharmaceutical industry. [0003] Taurine is widely distributed in the cell fluid of various tissues of animals, and is extremely rich in aquatic animals, especially marine animals. Studies have shown that marine organisms mussels contain a higher content of taurine, and the content of taurine in mussels accounts for about 3.9% of the total amino acids. The vast majority of taurine is distributed in cells, and the concen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C303/44C07C309/14
CPCC07C303/44C07C309/14
Inventor 朱正华沈妍彦
Owner ZHEJIANG ECONOMIC & TRADE POLYTECHNIC
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