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Pharmaceutical composition for improving immunity and promoting wound healing and preparation method thereof

A composition and drug technology, applied in the direction of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc., can solve the problems of no discovery, achieve the effect of treating skin damage, improving immunity, and good application value

Inactive Publication Date: 2021-07-27
北京岳昊科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a plant polysaccharide that can improve the body's immunity and treat skin damage has not been found so far.

Method used

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  • Pharmaceutical composition for improving immunity and promoting wound healing and preparation method thereof
  • Pharmaceutical composition for improving immunity and promoting wound healing and preparation method thereof
  • Pharmaceutical composition for improving immunity and promoting wound healing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation of embodiment 1 purple pearl grass active polysaccharide

[0020]Grind 10 g of the dried Asteraceae powder after freezing with liquid nitrogen, then place it in a round-bottomed flask, reflux with distilled water (1:20, m / v) at 75°C for 20 minutes, extract at 35°C, ultrasonic power 150W, Under the condition of extraction time of 15 minutes, ultrasonic assisted extraction for 15 minutes, the extract was filtered to remove impurities, concentrated to 1 / 3 of the original volume, added 85% ethanol at a volume ratio of 1:5, refrigerated and centrifuged for 10 minutes, and the precipitate was collected repeatedly to obtain vitality Polysaccharide and protein mixture, the precipitate was dissolved in distilled water, decolorized by activated carbon, deproteinized 5 times with n-butanol:chloroform=4:1, mL / mL, the supernatant was fixed to a 100mL volumetric flask, and placed in a dialysis bag (Molecular weight cut-off 3500Da) dialyzed for 72h. The dialyzed sampl...

Embodiment 2

[0024] Example 2 The effect of active polysaccharides on immunity

[0025] Experimental animals BALB / C mice, SPF grade, 60, were fed according to conventional feeding methods. The experimental group was randomly divided into 5 groups (blank control group, model group, low-dose active polysaccharide group, active polysaccharide dose group, and positive control group). After one week of adaptive feeding, they were administered by intragastric administration. The blank group and the model group were given intragastric administration of normal saline, the low-dose and high-dose groups were given intragastric administration of 100 and 500 mg / kg active polysaccharide, and the positive control group was intragastrically administered 40 mg / kg of levamisole hydrochloride, and the intragastric volume was 0.2 mL. Except for the blank control group, the other groups were intraperitoneally injected with cyclophosphamide 40 mg / kg once a day (to induce immune decline), and then gavaged the c...

Embodiment 3

[0033] Example 3 Polypeptide Promotes Research on Fibroblast Proliferation

[0034] Add the skin fibroblast suspension to the 96-well plate, 100 μL per well; put the 96-well plate into the cell incubator for culture, when the cells adhere to the wall and account for 90% or cover the bottom of the well, change the medium once and press The experimental design gradient was added to the peptide of SEQ ID NO: 1, so that the final concentration of the peptide was 0, 10, 20, 50 μg L -1 , set at least 3 replicate wells for each concentration. Take it out after 72 hours of drug action time, throw out all the liquid in the well, then add 100 μL of DMSO to each well, shake on the oscillator for 5 minutes, use a UV spectrophotometer to measure the absorbance (OD) value at a wavelength of 490nm, and calculate according to the formula Cell growth rate, cell growth rate=(OD drug / OD control)×100%. The results are shown in Table 3.

[0035] Table 3 Different concentrations of polypeptides ...

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Abstract

The invention relates to a pharmaceutical composition for improving immunity and promoting wound healing and a preparation method thereof. The active polysaccharide separated and prepared from the callicarpa bodinieri has the effect of improving the immunity, and the active polysaccharide and the prepared polypeptide have the effect of effectively treating skin injury while improving the immunity. The pharmaceutical composition has a good application value.

Description

technical field [0001] The invention relates to the field of biology, and more specifically relates to a pharmaceutical composition for improving immunity and promoting wound healing and a preparation method thereof. Background technique [0002] The skin is the largest organ of the human body. It regenerates quickly, is located on the surface of the body, and is prone to injury. After injury, the body undergoes a very coordinated healing process. Various cellular components, including inflammatory cells and tissue repair cells, participate in the healing process of skin wounds. The repair of skin damage needs to go through the strict arrangement, integration and differentiation, migration, proliferation and apoptosis of skin cells in order to achieve the regeneration of the multi-layered structure of the skin. [0003] Studies have found that fibroblasts are one of the main components of granulation tissue, and are also the main cells that synthesize and secrete extracellul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P17/02A61P37/04C08B37/00A61K31/715A61K36/85
CPCA61K31/715A61K36/85A61K38/16A61K2236/331A61K2236/53A61P17/02A61P37/04C08B37/0003A61K2300/00
Inventor 彭菲杨建华
Owner 北京岳昊科技发展有限公司
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