A continuous method for separating multiple mogrosinic acid monomers from Luo Han Guo root

A technology of Luo Han Guo and a continuous method, which is applied in the field of separation of natural triterpene acid monomers, can solve the problems of undisclosed and continuous acquisition of multiple high-content mogrosan fruit acid monomers, and achieve coherent processes, increased yields, and high yields Effect

Active Publication Date: 2022-05-27
HUNAN HUACHENG BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The above-mentioned prior art does not disclose a method for simultaneously and continuously obtaining multiple high-content mogrosinic acid monomers from the roots of Luo Han Guo

Method used

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  • A continuous method for separating multiple mogrosinic acid monomers from Luo Han Guo root
  • A continuous method for separating multiple mogrosinic acid monomers from Luo Han Guo root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] (1) Enzymolysis: take 10kg of dried Momordica grosvenori roots, pulverize to a particle size of 2-3mm, add 10kg of hydrochloric acid aqueous solution (pH=5), 0.02kg of sodium borate and 0.1kg of β-glucosidase, stir well, room temperature enzyme solution for 24 hours;

[0055] (2) Alkaline alcohol percolation: put the enzymatically hydrolyzed raw materials into the percolator, percolate and extract with 80L of alkaline methanol (the volume percentage concentration of methanol is 70%, which contains 1% sodium hydroxide), and percolation The flow rate is 1.0BV / hour, and the percolating liquid is obtained;

[0056] (3) Ceramic membrane filtration: filter the leachate with a ceramic membrane with a pore size of 50nm (filtration pressure 0.4Mpa) to obtain a ceramic membrane filtrate;

[0057] (4) decolorization: the ceramic membrane filtrate is passed through the alumina chromatography column (the consumption of alumina is 3kg, the height-diameter ratio of the chromatography...

Embodiment 2

[0064] (1) Enzymolysis: take 10kg of dried Momordica grosvenori roots, pulverize to a particle size of 2-3mm, add 15kg of dilute hydrochloric acid aqueous solution (pH=6), 0.05kg of sodium borate and 0.2kg of β-glucosidase, stir well, room temperature Enzymatic hydrolysis for 18 hours;

[0065] (2) Alkaline alcohol percolation: put the enzymatically hydrolyzed raw materials into the percolator, percolate and extract with 100L of alkaline ethanol (the volume percentage concentration of ethanol is 80%, which contains 0.8% potassium hydroxide), and percolation The flow rate is 1.5BV / hour, and the permeate is obtained;

[0066] (3) Ceramic membrane filtration: filter the leachate with a ceramic membrane with a pore size of 50nm (filtration pressure 0.4Mpa) to obtain a ceramic membrane filtrate;

[0067] (4) decolorization: the ceramic membrane filtrate is passed through the alumina chromatographic column (the consumption of alumina is 2kg, the height-diameter ratio of the chromat...

Embodiment 3

[0074] (1) Enzymolysis: take 10kg of dried Momordica grosvenori roots, pulverize to a particle size of 2-3mm, add 15kg of dilute sulfuric acid aqueous solution (pH=5), 0.03kg of sodium borate and 0.15kg of β-glucosidase, stir well, room temperature Enzymatic hydrolysis for 20 hours;

[0075] (2) Alkaline alcohol percolation: put the enzymatically hydrolyzed raw materials into the percolator, percolate and extract with 90L of alkaline methanol (the volume percentage concentration of methanol is 85%, which contains 1.5% of sodium hydroxide), and percolation The flow rate is 0.8BV / hour, and the percolating liquid is obtained;

[0076] (3) Ceramic membrane filtration: filter the leachate with a ceramic membrane with a pore size of 100 nm (filtration pressure 0.2 Mpa) to obtain a ceramic membrane filtrate;

[0077] (4) decolorization: the ceramic membrane filtrate is passed through the magnesia chromatography column (the consumption of magnesia is 2.6kg, the height-diameter ratio ...

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Abstract

The invention relates to a continuous method for separating multiple mogrosinic acid monomers from the roots of Luo Han Guo, which comprises the following steps: after the roots of Luo Han Guo are enzymatically hydrolyzed under weakly acidic conditions, they are extracted by percolating with an aqueous alcohol solution under alkaline conditions, and filtered , decolorized, concentrated, and the resulting concentrated solution was extracted under acidic conditions to obtain the total acid of Luo Han Guo, the total acid of Luo Han Guo was separated by gradient elution through chromatography, and the order of elution according to the polarity was Mo Han Guo Acid B, Mo Han Guo Acid A, Mo Han Guo Acid D and Luo Han Guo Acetate. The invention adopts enzymatic hydrolysis under alkaline conditions, carries out percolation extraction, and at the same time hydrolyzes mogroside to make it into free mogrosin, forms a salt and leaches, so as to increase the yield of mogrosin. The process of the present invention is coherent, simple, strong in operability, low in production cost, does not use toxic and harmful chemical solvents, is safe and environmentally friendly, has high product content and high yield, and is suitable for industrial production by extracting and separating the total acid of Luo Han Guo from the root of Luo Han Guo method.

Description

technical field [0001] The invention relates to a method for separating natural triterpene acid monomers, in particular to a continuous method for separating a variety of mogrosolic acid monomers from the roots of Luo Han Guo. Background technique [0002] Monk fruit is a precious local product in Guilin, mainly produced in Yongfu County and Longsheng County, and it is also one of the first batch of "medicine and edible" materials approved by the state. Mogroside is a unique natural high-intensity sweetener in Monk fruit, its sweetness is 300 times that of sucrose, and its calorie is zero. preventive effect. Mogroside is safe and non-toxic as a food. It is stipulated in the national mandatory standard "GB2760 Food Additive Use Standard" that Mogroside can be used in various foods in unlimited quantities. [0003] In the current production practice, the industrial production and utilization of Luo Han Guo mainly focus on the extraction and separation of glucosides in Luo Ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00C07J9/00C12P33/20C12P33/06
CPCC07J71/0005C07J9/005C12P33/20C12P33/06
Inventor 贺进军李伟黄华学黄俊宋谷良江小龙
Owner HUNAN HUACHENG BIOTECH
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