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High-efficiency crude heparin sodium production technology

A production process, the technology of heparin sodium, which is applied in the field of efficient crude heparin sodium production process, can solve the problems of long time-consuming production process of heparin sodium, and achieve the effects of accelerating the speed of enzymatic hydrolysis, reducing difficulty, increasing yield and production efficiency

Pending Publication Date: 2017-08-04
GUANGYUAN HAITIAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The object of the present invention is: in order to solve the problem that the existing heparin sodium production process takes a long time, the present invention provides a kind of efficient crude heparin sodium production process, which can shorten the production time of crude product heparin sodium and improve production efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The present embodiment provides a kind of efficient crude product heparin sodium production process, comprises the following steps:

[0040] (1) Raw material processing: After carefully washing pig intestines with clean water to remove internal and external dirt, mince them into minced meat;

[0041] (2) Ultrasonic enzymolysis extraction: add 4 times of water and 20% NaOH solution to adjust the pH to 7.5 in the minced pork intestine obtained in step (1), adjust the temperature to 36 ° C, add 0.5% protease, and Insulated and stirred in an ultrasonic oscillator for 15 minutes, filtered to obtain the enzymatic hydrolysis solution;

[0042] (3) Ion exchange adsorption: heat up the enzymolysis solution obtained in step (2) to 45°C, add D201 macroporous strong basic styrene anion exchange resin while stirring to adsorb the enzymolysis solution, when the added resin and When the surface of the enzymolysis solution is even, stop adding the resin, continue to stir for 2 hours, ...

Embodiment 2

[0047] The present embodiment provides a kind of efficient crude product heparin sodium production process, comprises the following steps:

[0048] (1) Raw material processing: After carefully washing pig intestines with clean water to remove internal and external dirt, mince them into minced meat;

[0049] (2) Ultrasonic enzymolysis extraction: add 6 times of water and 25% NaOH solution to adjust the pH to 8.5 in the minced pork intestine obtained in step (1), adjust the temperature to 40 ° C, add 1.5% protease, and Insulated and stirred in an ultrasonic oscillator for 20 minutes, filtered to obtain the enzymatic solution;

[0050] (3) Gel filtration chromatography: the enzymolysis solution obtained in step (2) is passed into a gel filtration chromatography column filled with glucose gel for chromatography, and the macromolecular chromatography liquid is discarded to obtain a small molecule chromatography liquid.

[0051] (4) Ion exchange adsorption: heat up the small molecul...

Embodiment 3

[0056] The present embodiment provides a kind of efficient crude product heparin sodium production process, comprises the following steps:

[0057] (1) Raw material processing: After carefully washing pig intestines with clean water to remove internal and external dirt, mince them into minced meat;

[0058] (2) Ultrasonic enzymolysis extraction: add 5 times of water and 22% NaOH solution to adjust the pH to 8 in the minced pork intestine obtained in step (1), adjust the temperature to 38 ° C, add 1.2% protease, and Insulated and stirred in an ultrasonic oscillator for 18 minutes, filtered to obtain the enzymatic hydrolysis solution;

[0059] (3) Gel filtration chromatography: the enzymolysis solution obtained in step (2) is passed into a gel filtration chromatography column filled with agarose gel for chromatography, and the macromolecular chromatography liquid is discarded to obtain a small molecule layer Analysis liquid.

[0060] (4) Ion exchange adsorption: heat up the sm...

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Abstract

The invention discloses a high-efficiency crude heparin sodium production technology, and relates to the technical field of medicine preparation. The technology comprises the following steps: raw material processing, ultrasonic enzymatic hydrolysis extraction, ion exchange adsorption, heparin elution and ethanol precipitation. The technology has the advantages of shortening of the production time of crude heparin sodium, increase of the production efficiency, increase of the yield of the crude heparin sodium, and suitableness for industrial promotion and production.

Description

technical field [0001] The invention relates to the technical field of medicine preparation, in particular to a high-efficiency crude heparin sodium production process. Background technique [0002] Cardiovascular and cerebrovascular diseases are the number one killer of human diseases. With the overnutrition brought about by the improvement of people's living standards, the deterioration of the global environment, the accelerated pace of life and the aging population, the global morbidity and mortality of cardiovascular and cerebrovascular diseases are increasing year by year. The emergence of heparin has created a miracle of life for many patients with cardiovascular and cerebrovascular diseases. [0003] Heparin is a compound with a relatively complex molecular structure, and it cannot be synthesized artificially in a short period of time. Currently, only heparin derived from the small intestinal mucosa of pigs can be used for clinical treatment. The raw material of hepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
CPCC08B37/0075C08B37/0003
Inventor 李海生
Owner GUANGYUAN HAITIAN IND
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