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A bioremediation method for immobilized petroleum-contaminated soil against cr(ⅵ) stress

A technology for contaminated soil, bioremediation, applied in the restoration of contaminated soil, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of reduced bioremediation efficiency, single, inorganic pollution, etc.

Active Publication Date: 2022-03-15
CHONGQING INST OF GEOLOGY & MINERAL RESOURCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, bioremediation technologies are often aimed at the remediation of soil contaminated by heavy metals or petroleum, but there are few technologies for the remediation of soil contaminated by heavy metals and petroleum at the same time.
However, in addition to serious petroleum pollutants, there are often inorganic pollution mainly heavy metals in polluted soil. As a result, in the bioremediation of petroleum-contaminated soil, petroleum-degrading bacteria are inhibited by heavy metals, and the bioremediation efficiency is remarkable. reduce

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The present embodiment is directed to the interior of Sichuan changning shale gas well field resistance to heavy metal contamination of petroleum hydrocarbons in soil fixing fossil oils Cr (Ⅵ) polluted soil remediation method stress soil research undertaken.

[0035] Using buckwheat shell as raw materials, the buckwheat shell successively washed with tap water and purified water, dried 105 deg.] C 12h, the dried raw materials were pulverized and sieved through a 10 mesh sieve. Gradually strengthened by means of four biochar reducing ability, first, taking the pulverized buckwheat shell into a reaction kettle while an aqueous solution of ethylenediamine was added 500mL mass fraction of 20% at 280 ℃, 5000kPa water conditions thermal carbonization 8h, filtered to give biochar, biochar and washed with 10% hydrochloric acid volume fraction. Second, the biochar cleaned was placed another reaction kettle equipped with 500mL volume fraction of 50% ascorbic acid solution, subjected ...

Embodiment 2

[0039] The present embodiment is directed to an anti-soil fixed oils, heavy metal contaminated soil fossil Tarim Oilfield undertaken Cr (Ⅵ) polluted soil remediation method stress development.

[0040] Using buckwheat shell as raw materials, the buckwheat shell washed with pure water, dried 105 deg.] C 8h, the dried raw materials were pulverized and sieved through a 10 mesh sieve. After taking the pulverized buckwheat shell placed in 1L reactor, taking mass fraction of 30% aqueous solution of ethylenediamine was added 1000mL reaction kettle carbide 12h, carbonization conditions 300 ℃, 7000kPa. Hou cleaning biochar carbide volume fraction of 12% hydrochloric acid, and clear the internal bore biochar. Biochar cleaned was placed another reaction kettle equipped with 1000mL volume fraction of 60% ascorbic acid solution in a second hydrothermal carbonization, carbonization conditions 250 ℃, 4000kPa, carbonization time of 8h. 30g of charcoal was added to the biological 300mL round botto...

Embodiment 3

[0044] This example is the R & D of the soil repair method of anti - heavy metal Cr (VI) stress in the Yangqing Oilfield Petroleum Pollution Soil.

[0045] Using the bitter seashes as raw materials, the bitter buckwheat sewage was washed with pure water and washed at 105 ° C for 8 h. In the same time, 500 ml of the dientine solution of 24% of the mass fraction was added at the same time, and the water was carbonized at 290 ° C, 6000 kPa, and the biocarbon was filtered, and the biocompancy was cleaned with a volume fraction of 10% hydrochloric acid. The second hydrocarbon carbonation was carbonized at 240 ° C, 2500 kPa, and the carbonization time was 6.5 h under 240 ° C, 2500 kPa. 300 ml of pure water was added to 500 ml of brown round bottom flask, and 20 g of bioaccharide was subjected to a secondary carbonized carbon, and the heat was rotated at 56 ° C, and 1 ml of 2 mol / L of formic acid was added dropwise between 5 min. The zero charge point is 2.5, and the redox reduction po...

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Abstract

The invention discloses a method for immobilized bioremediation of oil polluted soil against Cr(VI) stress. Using tartary buckwheat shells as raw materials, the reducibility of biochar was gradually improved by hydrothermal carbonization in ethylenediamine aqueous solution and ascorbic acid solution, rotating heating in formic acid, and loading nano-sized zero-valent aluminum, in order to achieve the reduction of Cr(Ⅵ). ; Enhance the detoxification ability of microorganisms by loading glutamylcysteine ​​synthetase and glutathione synthase; At the same time, the heavy metal Cr(Ⅵ) in the soil was partially removed.

Description

Technical field [0001] The present invention belongs to the field of environmental repair technology and the microbial fixation, which specifically relates to an anti-Cr (VI) stress of petroleum contamination soil immobilization biological repair method. Background technique [0002] With the expansion of the national oil and gas energy development scale, in the process of oil and gas energy, processing, transportation, transportation, transportation, and storage, the soil environment causes great pollution, contaminant-breaking rings soil microorganisms and plant cell membranes, inhibiting soil enzyme activities Even enter the food chain enriched to the human body, which causes extremely serious harm to human health. Biological repair is widely used in oil contamination soil repair due to its simple and convenient, low cost, no secondary pollution. CN110003913A loads magnesium oxide on the surface and interlayer between montmorillonite, mixing with bio-carbon, can have a good re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10C12N11/14C12R1/40
CPCB09C1/10C12N11/14
Inventor 任宏洋邓源鹏魏紫静龚星明侯迪雅孙雷涛王兵
Owner CHONGQING INST OF GEOLOGY & MINERAL RESOURCES
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