A bioremediation method for immobilized petroleum-contaminated soil against cr(ⅵ) stress
A technology for contaminated soil, bioremediation, applied in the restoration of contaminated soil, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of reduced bioremediation efficiency, single, inorganic pollution, etc.
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Embodiment 1
[0034] The present embodiment is directed to the interior of Sichuan changning shale gas well field resistance to heavy metal contamination of petroleum hydrocarbons in soil fixing fossil oils Cr (Ⅵ) polluted soil remediation method stress soil research undertaken.
[0035] Using buckwheat shell as raw materials, the buckwheat shell successively washed with tap water and purified water, dried 105 deg.] C 12h, the dried raw materials were pulverized and sieved through a 10 mesh sieve. Gradually strengthened by means of four biochar reducing ability, first, taking the pulverized buckwheat shell into a reaction kettle while an aqueous solution of ethylenediamine was added 500mL mass fraction of 20% at 280 ℃, 5000kPa water conditions thermal carbonization 8h, filtered to give biochar, biochar and washed with 10% hydrochloric acid volume fraction. Second, the biochar cleaned was placed another reaction kettle equipped with 500mL volume fraction of 50% ascorbic acid solution, subjected ...
Embodiment 2
[0039] The present embodiment is directed to an anti-soil fixed oils, heavy metal contaminated soil fossil Tarim Oilfield undertaken Cr (Ⅵ) polluted soil remediation method stress development.
[0040] Using buckwheat shell as raw materials, the buckwheat shell washed with pure water, dried 105 deg.] C 8h, the dried raw materials were pulverized and sieved through a 10 mesh sieve. After taking the pulverized buckwheat shell placed in 1L reactor, taking mass fraction of 30% aqueous solution of ethylenediamine was added 1000mL reaction kettle carbide 12h, carbonization conditions 300 ℃, 7000kPa. Hou cleaning biochar carbide volume fraction of 12% hydrochloric acid, and clear the internal bore biochar. Biochar cleaned was placed another reaction kettle equipped with 1000mL volume fraction of 60% ascorbic acid solution in a second hydrothermal carbonization, carbonization conditions 250 ℃, 4000kPa, carbonization time of 8h. 30g of charcoal was added to the biological 300mL round botto...
Embodiment 3
[0044] This example is the R & D of the soil repair method of anti - heavy metal Cr (VI) stress in the Yangqing Oilfield Petroleum Pollution Soil.
[0045] Using the bitter seashes as raw materials, the bitter buckwheat sewage was washed with pure water and washed at 105 ° C for 8 h. In the same time, 500 ml of the dientine solution of 24% of the mass fraction was added at the same time, and the water was carbonized at 290 ° C, 6000 kPa, and the biocarbon was filtered, and the biocompancy was cleaned with a volume fraction of 10% hydrochloric acid. The second hydrocarbon carbonation was carbonized at 240 ° C, 2500 kPa, and the carbonization time was 6.5 h under 240 ° C, 2500 kPa. 300 ml of pure water was added to 500 ml of brown round bottom flask, and 20 g of bioaccharide was subjected to a secondary carbonized carbon, and the heat was rotated at 56 ° C, and 1 ml of 2 mol / L of formic acid was added dropwise between 5 min. The zero charge point is 2.5, and the redox reduction po...
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