Application of PEG-modified black phosphorus quantum dots in evaluation of drug-induced acute kidney injury
A technology of acute kidney injury and quantum dots, applied in the field of biomedicine, can solve the problems of black phosphorus quantum dots being unstable, poorly compatible with biocompatibility, easy to be oxidized, etc., and achieve good results, increase yield, and evenly distribute the effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0043] Embodiment 1 prepares PEG-BPQDs:
[0044]A method for preparing PEG-modified black phosphorus quantum dots is characterized in that: the black phosphorus quantum dots are prepared by a liquid-phase ultrasonic etching method.
[0045] (1) Under ice bath conditions, take 5 mg of black phosphorus crystals (manufacturer: Jiangsu Xianfeng Nano Material Technology Co., Ltd.; NO: XF161; product number: 100944; batch number: 62897799), add 1 mL of saturated sodium hydroxide N-methyl Pyrrolidone (NMP) was ground in a mortar until the color of the liquid was black and turbid;
[0046] (2) The black turbid product obtained in step (1) is transferred to 3 mL of N-methylpyrrolidone (NMP) with saturated sodium hydroxide, so that the black phosphorus crystals are placed in N-methylpyrrolidone with saturated sodium hydroxide The concentration is 1.25mg / mL. Ultrasonication was performed with a 400W cell sonicator with a power of 350W and an ultrasonic amplitude of 80%, for 3 hours of ...
Embodiment 2
[0048] Embodiment 2 carries out morphology and property test to the PEG-BPQDs prepared in embodiment 1:
[0049] The BPQDs and PEG-BPQDs prepared in Example 1 were taken for ultraviolet absorption test, and the ultraviolet-visible absorption spectrum at 400-900 nm was measured using a quartz cuvette and using experimental water as a reference. figure 1 It is the ultraviolet absorption spectrogram of BPQDs and PEG-BPQDs, this phenomenon proves that the PEG-BPQDs prepared by the present invention has stronger light absorption properties than BPQDs, the measurement range of photoacoustics is 680~970nm, and PEG-BPQDs is at 680nm With UV absorption, it can be used for photoacoustic imaging.
[0050] Further, the morphology of the synthesized PEG-BPQDs was observed by transmission electron microscopy, and the supernatant of PEG-BPQDs was taken, and a small amount was dropped on the ultra-thin carbon film with a dropper, and allowed to dry naturally in the air. figure 2 It is a tra...
Embodiment 3
[0051] Embodiment 3 investigates the cytotoxicity of BPQDs prepared in Example 1 and PEG-BPQDs:
[0052] Human renal cortical proximal tubular epithelial cells HK-2 cells (Jiangsu Kaiji Biotechnology Co., Ltd.) and human breast cancer cells MCF-7 cells (Jiangsu Kaiji Biotechnology Co., Ltd.) were mixed at 1×10 per well. 4 Cells were inoculated into a 96-well plate with 200 μL of culture solution per well (edge wells were filled with sterile PBS), and cultured in an incubator for 24 h (37°C, 5% CO 2 ), observe the cell morphology under an inverted microscope to ensure that the cells are growing well. After aspirating the culture solution and washing it with PBS, add 20 μL of BPQDs prepared in Example 1 or PEG-BPQDs solution prepared in the present invention and 180 μL of medium with different concentration gradients (25, 50, 100, 150, 200 ppm), and continue to cultivate Incubate for 24 hours in the box. After incubation, add □200 μL tetramethylazolazolium blue (MTT) solutio...
PUM
Property | Measurement | Unit |
---|---|---|
Particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
![application no application](https://static-eureka-patsnap-com.libproxy1.nus.edu.sg/ssr/23.2.0/_nuxt/application.06fe782c.png)
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap