Application of PEG-modified black phosphorus quantum dots in evaluation of drug-induced acute kidney injury

A technology of acute kidney injury and quantum dots, applied in the field of biomedicine, can solve the problems of black phosphorus quantum dots being unstable, poorly compatible with biocompatibility, easy to be oxidized, etc., and achieve good results, increase yield, and evenly distribute the effect

Active Publication Date: 2021-06-18
CHINA PHARM UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the technical problems of black phosphorus quantum dot instability, easy oxidation in water, and poor biocompatibility in the prior art, the present invention provides a polyethylene glycol-modified black phosphorus quantum dot and its preparation method and its use in Application in drug-induced acute kidney injury

Method used

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  • Application of PEG-modified black phosphorus quantum dots in evaluation of drug-induced acute kidney injury
  • Application of PEG-modified black phosphorus quantum dots in evaluation of drug-induced acute kidney injury
  • Application of PEG-modified black phosphorus quantum dots in evaluation of drug-induced acute kidney injury

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1 prepares PEG-BPQDs:

[0044]A method for preparing PEG-modified black phosphorus quantum dots is characterized in that: the black phosphorus quantum dots are prepared by a liquid-phase ultrasonic etching method.

[0045] (1) Under ice bath conditions, take 5 mg of black phosphorus crystals (manufacturer: Jiangsu Xianfeng Nano Material Technology Co., Ltd.; NO: XF161; product number: 100944; batch number: 62897799), add 1 mL of saturated sodium hydroxide N-methyl Pyrrolidone (NMP) was ground in a mortar until the color of the liquid was black and turbid;

[0046] (2) The black turbid product obtained in step (1) is transferred to 3 mL of N-methylpyrrolidone (NMP) with saturated sodium hydroxide, so that the black phosphorus crystals are placed in N-methylpyrrolidone with saturated sodium hydroxide The concentration is 1.25mg / mL. Ultrasonication was performed with a 400W cell sonicator with a power of 350W and an ultrasonic amplitude of 80%, for 3 hours of ...

Embodiment 2

[0048] Embodiment 2 carries out morphology and property test to the PEG-BPQDs prepared in embodiment 1:

[0049] The BPQDs and PEG-BPQDs prepared in Example 1 were taken for ultraviolet absorption test, and the ultraviolet-visible absorption spectrum at 400-900 nm was measured using a quartz cuvette and using experimental water as a reference. figure 1 It is the ultraviolet absorption spectrogram of BPQDs and PEG-BPQDs, this phenomenon proves that the PEG-BPQDs prepared by the present invention has stronger light absorption properties than BPQDs, the measurement range of photoacoustics is 680~970nm, and PEG-BPQDs is at 680nm With UV absorption, it can be used for photoacoustic imaging.

[0050] Further, the morphology of the synthesized PEG-BPQDs was observed by transmission electron microscopy, and the supernatant of PEG-BPQDs was taken, and a small amount was dropped on the ultra-thin carbon film with a dropper, and allowed to dry naturally in the air. figure 2 It is a tra...

Embodiment 3

[0051] Embodiment 3 investigates the cytotoxicity of BPQDs prepared in Example 1 and PEG-BPQDs:

[0052] Human renal cortical proximal tubular epithelial cells HK-2 cells (Jiangsu Kaiji Biotechnology Co., Ltd.) and human breast cancer cells MCF-7 cells (Jiangsu Kaiji Biotechnology Co., Ltd.) were mixed at 1×10 per well. 4 Cells were inoculated into a 96-well plate with 200 μL of culture solution per well (edge ​​wells were filled with sterile PBS), and cultured in an incubator for 24 h (37°C, 5% CO 2 ), observe the cell morphology under an inverted microscope to ensure that the cells are growing well. After aspirating the culture solution and washing it with PBS, add 20 μL of BPQDs prepared in Example 1 or PEG-BPQDs solution prepared in the present invention and 180 μL of medium with different concentration gradients (25, 50, 100, 150, 200 ppm), and continue to cultivate Incubate for 24 hours in the box. After incubation, add □200 μL tetramethylazolazolium blue (MTT) solutio...

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Abstract

The invention discloses a method for evaluating drug-induced acute kidney injury by using PEG-modified black phosphorus quantum dots, the black phosphorus quantum dots are prepared by using a liquid phase ultrasonic etching method, the biocompatibility of the black phosphorus quantum dots is improved through PEG modification, PEG-BPQDs have a good photoacoustic imaging effect, and the particle size is less than 5nm, so that the PEG-BPQDs can pass through the glomerular filtration membrane, and can be used as a photoacoustic imaging contrast agent for evaluating the drug-induced acute kidney injury. The PEG-BPQDs have the advantages that the PEG-BPQDs not only have very high kidney removal efficiency and good biocompatibility, but also can noninvasively monitor the drug-induced acute kidney injury conditions of living mice in real time by adopting a photoacoustic imaging system, and have a wide application prospect in the aspect of biological imaging.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of PEG-modified black phosphorus quantum dots in evaluating drug-induced acute kidney injury. Background technique [0002] Drug-induced acute kidney injury (DIKI) is a common clinical syndrome. Currently, the clinical detection of drug-induced acute kidney injury mainly relies on blood creatinine, blood urea nitrogen, and renal biopsy. Detection methods There are disadvantages such as poor specificity, detection hysteresis, large trauma, and easy to cause various complications. Therefore, how to better judge the occurrence and evaluation of drug-induced acute kidney injury has become an important challenge. At present, it is urgent to develop a better evaluation method for real-time, in situ, and highly sensitive diagnosis of drug-induced acute kidney injury. damage assessment. [0003] Photoacoustic imaging is a composite biological imaging mode that combines the ad...

Claims

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Application Information

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IPC IPC(8): A61K49/22C01B25/00C01B25/02B82Y40/00B82Y20/00
CPCA61K49/221C01B25/003C01B25/02B82Y40/00B82Y20/00
Inventor 田蒋为张王宁吴阳吴燕沈卓霞
Owner CHINA PHARM UNIV
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