Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nano assembly co-loaded with rapamycin and curcumin, preparation method and application thereof

A nano-assembly and rapamycin technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., to achieve strong cell affinity, reduce total protein content, and improve lung inflammation

Active Publication Date: 2021-06-15
WEST CHINA HOSPITAL SICHUAN UNIV
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the therapeutic effect of curcumin and rapamycin on acute lung injury needs to be further improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nano assembly co-loaded with rapamycin and curcumin, preparation method and application thereof
  • Nano assembly co-loaded with rapamycin and curcumin, preparation method and application thereof
  • Nano assembly co-loaded with rapamycin and curcumin, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation method of embodiment 1 rapamycin / curcumin-IR806 nano assembly

[0042] Accurately weigh rapamycin and curcumin according to the mass ratio of 2:1, dissolve in DMSO to prepare a solution with a total drug concentration of 6mg / ml as the oil phase; weigh IR806 and dissolve it in deionized water to prepare a water with a concentration of 1mg / ml phase; 1ml of oil phase was added dropwise to 10ml of water phase, and after 60min of magnetic stirring at room temperature in the dark, it was dialyzed with deionized water (dialysis membrane M W : 1000) to remove DMSO by dialyzing for 6 hours, then collect by ultracentrifugation and disperse to prepare drug-loaded IR806 nano-assemblies, and store them at 4°C.

Embodiment 2

[0043] Example 2 The preparation method of rapamycin / curcumin-IR820 nano assembly

[0044]Accurately weigh rapamycin and curcumin according to a mass ratio of 1:1, dissolve them in ethanol to prepare a solution with a total drug concentration of 6mg / ml as the oil phase; weigh IR820 and dissolve it in deionized water to prepare a water solution with a concentration of 1mg / ml phase; 1ml of the oil phase was added dropwise to 6ml of the water phase, and after magnetic stirring for 120min at room temperature in the dark, it was dialyzed with deionized water (dialysis membrane M W : 1000) to remove ethanol by dialysis for 4 hours, and then the resulting system was subjected to ultrafiltration (ultrafiltration membrane molecular weight cut-off 1000) to intercept nanoparticles, and then the drug-loaded IR820 nano-assembly was prepared and stored at 4°C.

Embodiment 3

[0045] Example 3 Preparation method of rapamycin / curcumin-IR783 lipid nano-assembly

[0046] Accurately weigh rapamycin and curcumin according to a mass ratio of 1:3, dissolve them in DMSO / ethanol (V:V=1:1) mixed solvent, prepare a solution with a total drug concentration of 10mg / ml as the oil phase; weigh IR783 was dissolved in deionized water to prepare a water phase with a concentration of 1mg / ml; 1ml of the oil phase was added dropwise to 8ml of the water phase, stirred at room temperature and protected from light for 90min by magnetic force, and then dialyzed with deionized water (dialysis membrane M W : 1000) dialyzed for 8h to remove DMSO and ethanol, then collected by ultracentrifugation and dispersed to prepare drug-loaded IR783 nano-assemblies.

[0047] The phospholipid mixture obtained by mixing 7 mg of dipalmitoylphosphatidylcholine and 3 mg of distearoylphosphatidylethanolamine-polyethylene glycol 2000 was dissolved in 2.5 mL of chloroform, and the system was vacu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a nano assembly co-loaded with rapamycin and curcumin, a preparation method and application thereof. In the nano assembly, the mass ratio of the rapamycin to the curcumin is (3.0-4.0): 1. Compared with free rapamycin and free curcumin, the nano assembly can effectively regulate and control the conversion of macrophages from an inflammatory M1 state to an anti-inflammatory M2 state, improves lung inflammation caused by acute lung injury and reduce the content of total protein in the lungs of mice with the acute lung injury, has a synergistic effect on treatment of the acute lung injury, and provides a new choice for preparing a medicine for treating the acute lung injury.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular relates to a nano assembly co-carrying rapamycin and curcumin, a preparation method and application thereof. Background technique [0002] Acute lung injury (ALI) is a relatively common clinical emergency and critical illness. It is an acute and progressive hypoxic respiratory failure caused by various pathogenic factors inside and outside the lungs other than cardiogenic. The severe stage of ALI can develop into acute respiratory distress syndrome (ARDS). Clinically, the mortality rate of ALI induced by pneumonia or sepsis is very high, and the quality of life of surviving patients is also seriously affected due to severe impairment of lung function. Although there are many studies on acute lung injury, the current clinical treatment of ALI is relatively single, such as the use of broad-spectrum antibiotics, ventilator-assisted ventilation and maintenance of hemodynamics, etc., and the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127B82Y40/00B82Y5/00A61K31/436A61K31/12A61K47/22A61P11/00
CPCA61K9/127B82Y40/00B82Y5/00A61K31/436A61K31/12A61K47/22A61P11/00A61K2300/00
Inventor 彭锦荣杨倩肖海涛许学文
Owner WEST CHINA HOSPITAL SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com