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Preparation of Ce6 loaded on lipid-coated calcium carbonate carrier as well as preparation method and application of preparation

A technology of cc-ce6 and sodium carbonate aqueous solution, which is applied in the directions of medical preparations containing active ingredients, pharmaceutical formulas, antitumor drugs, etc.

Pending Publication Date: 2021-06-11
HAINAN HUIYUANTANG PHARMA +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, currently available cancer vaccines often require complex processing of blood products to provide antigens / adjuvants to meet the vaccination requirements of subjects

Method used

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  • Preparation of Ce6 loaded on lipid-coated calcium carbonate carrier as well as preparation method and application of preparation
  • Preparation of Ce6 loaded on lipid-coated calcium carbonate carrier as well as preparation method and application of preparation
  • Preparation of Ce6 loaded on lipid-coated calcium carbonate carrier as well as preparation method and application of preparation

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Experimental program
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Effect test

Embodiment L

[0050] The preparation of embodiment Li / CC-Ce6 nanoparticle

[0051] CaCl 2 and Ce6 dispersed in water-in-oil microemulsion. Also prepared the same containing Na 2 CO 3 and DOPA microemulsions. The two solutions were mixed for 20 minutes to prepare hydrophobic DOPA-modified CC-Ce6, which was precipitated with absolute ethanol and centrifuged (CR22, Hitachi, Japan). After sufficiently washing with ethanol, the DOPA-modified CC-Ce6 was mixed with an appropriate amount of DOPC, FA-DSPE-PEG and cholesterol in a mixed solution of chloroform and ethanol (1:1, v / v). After evaporating the solvent by steam, the formed film was dispersed in medium PBS to obtain a Li / CC-Ce6 solution. Specific steps include:

[0052] 1) Preparation of calcium chloride solution: take 55.49 grams of calcium chloride, add distilled water to dissolve, and configure 2mol / L calcium chloride solution in a 250ml volumetric flask;

[0053] 2) Preparation of chlorin e6 solution: Weigh 156.18 grams of chlorin...

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Abstract

The invention relates to the technical field of pharmaceutical preparations, in particular to a lipid-coated calcium carbonate carrier Ce6-loaded preparation as well as a preparation method and application thereof. The Li / CC-Ce6 provided by the invention has good stability, biocompatibility and tumor targeting property, and can effectively generate ROS (reactive oxygen species) under laser stimulation. ROS can trigger apoptosis to expose TAA, and is expected to recruit DC into tumor tissue through an inflammation simulation mechanism. The DCs are activated in situ to release the corresponding inflammation (including IL-6 and TNF-alpha) and trigger a subsequent immune response cascade reaction. In-vitro and in-vivo experiments show that the platform has a strong capability of enhancing DC vaccination, and can effectively inhibit the growth of primary and distant growing tumors, which may be a new method for effectively treating cancers.

Description

technical field [0001] The invention relates to the technical field of pharmaceutical preparations, in particular to a preparation of lipid-coated calcium carbonate carrier loaded with Ce6, a preparation method and application thereof. Background technique [0002] Immunotherapy for cancer is a novel class of therapies that includes cancer vaccines, immune checkpoint therapies, and other T-cell therapies. In particular, cancer vaccines with irreplaceable advantages (such as simple preparation and low cost) compared with other methods are widely regarded as potential cancer treatments. Recent studies have gradually revealed the key functions of dendritic cells (DCs) in activating T cells and triggering immune responses, which are essential for immunotherapy. Multiple previous studies have focused on finding vaccines for cancer immunotherapy. However, currently available cancer vaccines often require complex processing of blood products to provide antigens / adjuvants to meet ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/02A61P35/00
CPCA61K41/0071A61K47/02A61P35/00
Inventor 戴文鑫徐朗
Owner HAINAN HUIYUANTANG PHARMA
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