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A reaction product of dopamine and resorcinol, its preparation method and application

A technology of reaction product, resorcinol, applied in the field of analytical chemistry, can solve the problems of death, hereditary gene defect, irritation and nasal septum perforation, and achieve the effect of sensitive detection, good linear relationship, and efficient detection.

Active Publication Date: 2022-04-15
CHONGQING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the human body, hexavalent chromium (Cr 6+ ) is an ingestion poison / inhalation poison, skin contact may cause allergies; it is more likely to cause genetic defects, and inhalation may cause cancer; it will also cause many other health problems, such as inhalation of certain higher concentrations of hexavalent Chromium (Cr 6+ ) compounds can cause runny nose, sneezing, itching, epistaxis, ulcers, and perforation of the nasal septum; short-term high-dose exposure can have adverse consequences at the site of exposure, including ulceration, irritation of the nasal mucosa, and perforation of the nasal septum; ingestion of very large doses of chromium Can cause kidney and liver damage, nausea, gastrointestinal irritation, stomach ulcers, convulsions, and death; skin contact can cause ulcers or allergic reactions

Method used

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  • A reaction product of dopamine and resorcinol, its preparation method and application
  • A reaction product of dopamine and resorcinol, its preparation method and application
  • A reaction product of dopamine and resorcinol, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] An organic fluorescent molecular reaction product formed by the reaction of dopamine and resorcinol, wherein the structural formula of the product is:

[0038] The main reaction formula in the preparation process is:

[0039] Concrete reaction process is as follows:

[0040] The dopamine and resorcinol are added into the neutral solution with pH=7 according to the molar ratio of 1:1, stirred and mixed and reacted to obtain the reaction product (probe).

[0041] The above preparation process is derived from the reference "Unimolecular Variant of the Fluorescence Turn-On Oxidative Coupling of Catecholamines with Resorcinols, ACS Omega 2019, 4, 1541-1548", and the synthesized product is also consistent with the product in the literature.

Embodiment 2

[0043] To the reaction product (probe) prepared in Example 1, in the reaction product (probe), add hexavalent chromium ion (Cr 6+ ) and ascorbic acid to form a mixture for fluorescence performance detection, mainly under the excitation of ultraviolet light with a wavelength of 420nm for fluorescence detection, the specific method is:

[0044] The reaction product (probe) prepared in Example 1 was dissolved in 40 mM PBS buffer solution, and the pH of the solution was adjusted to 11 to form a reaction product solution (probe solution) with a concentration of 10 μM, which was excited by ultraviolet light with a wavelength of 420 nm Test its fluorescence intensity at 25°C; then continue to add potassium dichromate to the above 10 μM reaction product solution (probe solution) to form a reaction product and chromium ion mixed solution (probe+Cr 6+ ), wherein the pH=11, Cr of the mixed solution are still adjusted 6+ The concentration is 1mM, and its fluorescence intensity is tested ...

Embodiment 3

[0047] The detection of hexavalent chromium ion (Cr) by fluorescence quenching method under different test conditions 6+ )Impact:

[0048] 1. Hexavalent chromium ion (Cr 6+ ) on the fluorescence intensity of the reaction product (probe)

[0049] The reaction product (probe) was added to the PBS buffer solution to form a reaction product solution (probe solution) with a concentration of 10 μM, and then divided into the same three solutions, and dichromic acid was added at 25°C, 37°C and 50°C respectively Potassium forms hexavalent chromium ions (Cr 6+ ), adjust the pH=11 of the solution in the whole process, test the fluorescence intensity before and after adding potassium dichromate under the ultraviolet light excitation of 420nm, compare the fluorescence intensity at the 460nm place at the emission wavelength, the result is as follows figure 2 shown. From figure 2 It can be seen that the fluorescence intensity at 460nm of the reaction product (probe) obtained by testin...

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Abstract

The invention relates to a reaction product of dopamine and resorcinol, a preparation method and application thereof, and belongs to the technical field of analytical chemistry. The present invention mainly forms a fluorescent probe (probe) with good water solubility through the reaction of dopamine and resorcinol. The probe disclosed by the present invention and Cr 6+ The absorption wavelength (430 ~ 550nm) overlaps, Cr 6+ Inner filter effect is produced between dopamine and resorcinol reaction product provided by the present invention, the fluorescence intensity decreases with the increase of the hexavalent chromium concentration added, and maintains a good linear relationship; simultaneously because ascorbic acid can convert Cr 6+ reduced to Cr 3+ , thus destroying Cr 6+ The internal filter effect between the reaction product and the reaction product of dopamine and resorcinol is affected by Cr 6+ The reduced fluorescence intensity is restored. Therefore dopamine provided by the invention and resorcinol reaction product are to hexavalent chromium ion (Cr 6+ ) and ascorbic acid have green, efficient and sensitive detection.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a reaction product of dopamine and resorcinol, a preparation method and application thereof. Background technique [0002] Heavy metal ions are very harmful to the ecological environment and human health, among which hexavalent chromium (Cr 6+ ) is extremely harmful. For the human body, hexavalent chromium (Cr 6+ ) is an ingestion poison / inhalation poison, skin contact may cause allergies; it is more likely to cause genetic defects, and inhalation may cause cancer; it will also cause many other health problems, such as inhalation of certain higher concentrations of hexavalent Chromium (Cr 6+ ) compounds can cause runny nose, sneezing, itching, epistaxis, ulcers, and perforation of the nasal septum; short-term high-dose exposure can have adverse consequences at the site of exposure, including ulceration, irritation of the nasal mucosa, and perforation o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D491/08C09K11/06G01N21/64
CPCC07D491/08C09K11/06G01N21/643G01N2021/6432C09K2211/1033
Inventor 祁文静吴狄陈蕾
Owner CHONGQING NORMAL UNIVERSITY
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