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Anti-adhesion composite multi-layer amnion material

A technology for preventing adhesion and amniotic membrane, applied in the field of composite multi-layer amniotic membrane materials, can solve the problems of low mechanical strength, easy slippage, easy tearing, etc., to achieve the effect of improving mechanical strength and solving easy tearing

Inactive Publication Date: 2021-04-30
TANGSHAN WORKERS HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the shortcomings of amniotic membrane products in the prior art, such as brittle quality, easy to tear, low mechanical strength, and easy slippage during operation, and propose a composite multi-layer amniotic membrane material that prevents adhesion

Method used

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  • Anti-adhesion composite multi-layer amnion material

Examples

Experimental program
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Effect test

preparation example Construction

[0019] The preparation of freeze-dried amniotic membrane: put the fresh amniotic membrane in the working room of the freeze dryer, pre-freeze at low temperature for several hours, turn on the condensation chamber to refrigerate, make the temperature of the cold trap reach -10℃~-56℃, turn on the vacuum pump, vacuum freeze-dry, water content is reduced to below 20%. Freeze-dried amnion was sterilized by cobalt 60 irradiation.

[0020] Preparation of electrospun amniotic matrix nanofibrous membrane: Prepare polycaprolactone (PCL) spinning liquid, stir and mix overnight with a magnetic bar. Set various parameters such as ambient temperature and humidity, and connect the electrospinning device. Put the electrospinning solution into the syringe, adjust the flow rate of the solution, high voltage, and adjust the receiving distance to receive the PCL fiber membrane on the two surfaces of the lyophilized amniotic membrane. The prepared nanofiber membrane was dried overnight in a vacu...

Embodiment

[0021] Example: after blunt separation between the amnion and chorion to obtain smooth, translucent fresh amnion, soak it in a balanced salt solution containing 50ug / ml penicillin and 50ug / ml streptomycin for 20 minutes, and place it in DMEM medium Store in the refrigerator at 4°C until use. Put the fresh amniotic membrane in the working room of the freeze dryer, pre-freeze at 0°C to -50°C for 1 to 10 hours, turn on the condensation chamber to cool down, make the temperature of the cold trap reach -10°C to -56°C, turn on the vacuum pump, and vacuum freeze dry. The moisture content ranges from 1% to 20%. Freeze-dried amnion was sterilized by cobalt 60 irradiation. Prepare polycaprolactone (PCL) spinning solution, stir and mix overnight with a magnetic bar. Set the ambient temperature to 25°C and the humidity to 60%, and connect the electrospinning device. Put the electrospinning solution into a 2ml syringe with a needle with a diameter of 0.7mm, adjust the flow rate of the s...

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Abstract

The invention relates to the field, in particular to an anti-adhesion composite multi-layer amnion material, which comprises a fresh amnion, an epithelial cell layer, a basement membrane and the like, the fresh amnion is subjected to freezing and vacuum drying treatment, the moisture content of the fresh amnion ranges from 1% to 20%, and the epithelial cell layer contains collagen matrixes and various active proteins. Through a high-voltage electrospinning technology, two surfaces of a freeze-dried amniotic membrane are used for receiving polycaprolactone fibrous membranes to form a multilayer composite amnion, and a single surface (epithelial cell surface or basal membrane surface) of the freeze-dried amnion is used for receiving PCL fibrous membranes to form a double-layer composite membrane. Amnion, PCL and other macromolecular substances are blended to form multi-layer nanofibers through the high-voltage electrostatic spinning technology, the mechanical strength and hydrophobicity of an amnion matrix are improved, a growth factor slow-release system conforming to the tendon healing cycle is constructed, and the defects that an amnion product is prone to tearing, low in mechanical strength, extremely prone to slipping during application in an operation and the like are effectively overcome. A new way is provided for clinically solving the problem of tendon adhesion.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a composite multilayer amniotic membrane material for preventing adhesion. Background technique [0002] With social progress and industrial development, tendon injuries caused by trauma are very common. Adhesion between tendon and surrounding tissue is the most common complication after tendon repair. So far, there is still no effective solution, and it has become a clinical problem that needs to be solved urgently. Through the in-depth study of the formation mechanism of tendon adhesions, many methods and materials to prevent tendon adhesions have been explored. The application of physical barriers to block exogenous healing is currently the main method to prevent tendon adhesions. The non-biological materials used in the early stage, such as silica gel, gold foil, and autologous fascia, have many shortcomings and have been gradually eliminated. Absorbable macromolec...

Claims

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Application Information

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IPC IPC(8): A61L31/12A61L31/06A61L31/00A61L31/14D04H1/435D04H1/728
CPCA61L31/005A61L31/06A61L31/14A61L31/148A61L2300/424A61L2400/12D04H1/435D04H1/728
Inventor 刘春杰田德虎白江博于昆仑
Owner TANGSHAN WORKERS HOSPITAL
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