Method for detecting diethylstilbestrol residues in milk and milk powder

A technology for diethylstilbestrol and residues, which is applied in the field of detection and analysis, can solve the problems that liquid chromatography cannot be accurately qualitative, the pretreatment process is cumbersome, and the detection sensitivity is low, and the effects of saving test costs, improving method recovery and high sensitivity are achieved.

Inactive Publication Date: 2021-04-20
山东欧陆检测有限公司
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Problems solved by technology

[0003] The existing instrumental analysis and detection methods for diethylstilbestrol in milk and milk powder include liquid chromatography and liquid chromatography-tandem mass spectrometry. Liquid chromatography cannot be accurately qualitative and has low detection sensitivity. It is cumbersome and time-consuming. According to GB / T 22992-2008 "Determination of zearalanol, zearalane, diethylstilbestrol, hexestrol, and dienestrol residues in milk and milk powder, liquid chromatography-tandem mass spectrometry "For example, the pretreatment process requires sodium hydroxide to adjust the pH, and then it is purified by an anion solid phase extraction column, which takes a long time and the cost of consumables is high.

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  • Method for detecting diethylstilbestrol residues in milk and milk powder
  • Method for detecting diethylstilbestrol residues in milk and milk powder
  • Method for detecting diethylstilbestrol residues in milk and milk powder

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[0048] The present invention will be further explained through specific embodiments below, however, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described here. Rather, these embodiments are provided for more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0049] "Includes" or "comprises" mentioned throughout the specification and claims is an open term, so it should be interpreted as "including but not limited to". The subsequent description in the specification is a preferred implementation mode for implementing the present invention, but the description is for the purpose of the general principles of the specification, and is not intended to limit the scope of the present invention. The scope of protection of the present invention should be defined by the appended claims.

[0050] 1. Selected reagents ...

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Abstract

The invention provides a method for detecting diethylstilbestrol residues in milk and milk powder. The method comprises the following steps: pretreating a to-be-detected sample, and then carrying out HPLC-MS / MS detection; and carrying out acidification acetonitrile extraction, QuEChERS technology purification and redissolution on a target compound, and directly measuring on a machine, wherein the pretreatment operation is simple. According to the invention, the optimized method is low in matrix effect, and experimental steps are simplified; and the method is simple, convenient and sensitive, and is suitable for measuring the diethylstilbestrol residual quantity in milk and milk powder, so that the method has a good practical application value.

Description

technical field [0001] The invention belongs to the technical field of detection and analysis, and in particular relates to a method for detecting residual diethylstilbestrol in milk and milk powder based on high performance liquid chromatography tandem mass spectrometry. Background technique [0002] Diethylstilbestrol (DES) is a synthetic estrogen hormone that can produce all the same pharmacological and therapeutic effects as natural estradiol. In the breeding industry, diethylstilbestrol was once added to the feed as a growth promoter to stimulate the normal production and metabolism of dairy cows, increase chlorine retention in dairy cows, increase the speed of amino acid synthesis of protein in dairy cows, and finally achieve the purpose of increasing milk yield. Studies have shown that diethylstilbestrol, which remains in animal-derived foods and enters the human body through the food chain, is an environmental endocrine disruptor, which can inhibit the secretion of p...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
Inventor 高素兰
Owner 山东欧陆检测有限公司
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