Method for detecting content of water-soluble calcium in dried oysters

A water-soluble calcium and detection method technology, which is applied in the field of inspection, can solve the problems of difficulty in unifying the detection accuracy of calcium ions, difficulty in meeting practical requirements, and large loss of raw materials, so as to avoid loss of activity, good development prospects, and gentle and fast process. Effect

Inactive Publication Date: 2021-04-20
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But these methods all have certain flaws: when adopting spectrophotometric method to detect calcium content, there is the sample dilution treatment error; The potassium permanganate method used to indirectly measure the calcium ion content uses a large loss of raw materials, and the pre-treatment of raw materials causes large irreparable deviations
Due to the different sample processing techniques and determination methods of these methods, it is difficult to unify the accuracy of calcium ion detection. Therefore, a method with faster detection and more accurate results is urgently needed to detect the water-soluble calcium content in dried oysters.

Method used

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  • Method for detecting content of water-soluble calcium in dried oysters
  • Method for detecting content of water-soluble calcium in dried oysters
  • Method for detecting content of water-soluble calcium in dried oysters

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Preparation of fluorescent probe standard curve

[0043] 1. Preparation of medicines

[0044] (1) 1M 3-morpholine propanesulfonic acid (MOPS) buffer solution: Weigh 20.926g MOPS, add 80mL deionized water to dissolve, adjust the pH value of the solution to 12 with 2M NaOH, and finally add deionized water to dilute to 100 mL, you can get 1M MOPSBuffer (pH=12) buffer.

[0045] (2) 10 mM calcein fluorescent probe: Weigh 0.006205 g of calcein trisodium salt and dissolve it in 1 mL of dimethyl sulfoxide (DMSO).

[0046] (3) 0.1M nitrilotriacetic acid (NTA) solution: Weigh 1.9114g nitrilotriacetic acid (NTA) and dissolve it in 100mL deionized water.

[0047] (4) 0.1M CaCl 2 Solution: Weigh 1.1098g of calcium chloride and dissolve it in 100mL of distilled water. 2. Ca with different concentration gradients 2+ Preparation of the buffer system:

[0048] Take 0.1M CaCl 2Solution 0μL, 50μL, 100μL, 500μL, 1.25mL, 1.5mL, 2.0mL, 2.5mL, 3.5mL, 4.0mL, 4.5mL, 4.75mL, 4.95mL, 4.975...

Embodiment 2

[0070] A method for detecting water-soluble calcium content in dried oysters, comprising the steps of:

[0071] A1. Sample pretreatment: Cut the dried oysters into 2-3 mm long pieces for subsequent grinding; put the cut dried oysters into an oven, adjust the temperature of the oven to 60°C, and dry for 2 hours until the surface is crisp; Put the dried sample into an ultra-centrifugal grinder for crushing, and then use a 100-mesh sieve ring to grind repeatedly until the powder is uniform and has no obvious large particles;

[0072] A2. Microwave-ultrasonic collaborative extraction: take 10g of ground dried oyster powder, add deionized water according to the ratio of solid to liquid ratio of 1:3 for mixing, put it into the extraction instrument, and perform microwave-ultrasonic collaborative extraction to obtain the extract . The microwave power is 300W, the ultrasonic power is 1500W, the extraction temperature is 50°C, and the ultrasonic treatment is continued for 5 minutes. ...

Embodiment 3

[0075] A method for detecting water-soluble calcium content in dried oysters, comprising the steps of:

[0076] A1. Sample pretreatment: Cut the dried oysters into 2-3 mm long pieces for subsequent grinding; put the cut dried oysters into an oven, adjust the temperature of the oven to 60°C, and dry for 3 hours until the surface is crisp; Put the dried sample into an ultra-centrifugal grinder for crushing, and then use a 100-mesh sieve ring to grind repeatedly until the powder is uniform and has no obvious large particles;

[0077] A2. Microwave-ultrasonic collaborative extraction: Take 10g of ground dried oyster powder, add deionized water according to the ratio of solid to liquid ratio of 1:6 for mixing, put it into the extraction instrument, and perform microwave-ultrasonic collaborative extraction to obtain the extract . The microwave power is 300W, the ultrasonic power is 1500W, the extraction temperature is 70°C, and the ultrasonic treatment is continued for 5 minutes. ...

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Abstract

The invention discloses a method for detecting the content of water-soluble calcium in dried oysters, and belongs to the technical field of inspection. The method comprises the following steps: A1, pretreating a sample: cutting the dried oysters into small sections, baking the small sections in a baking oven until the surfaces of the small sections are dry and crisp, taking out the small sections, crushing the small sections in an ultra-centrifugal grinder, and sieving the crushed small sections; A2, microwave-ultrasonic synergistic extraction: mixing the ground dried oyster powder with deionized water according to a solid-to-liquid ratio of 1: (3-12), and then carrying out microwave-ultrasonic synergistic extraction to obtain an extracting solution; A3, performing detecting by a fluorescence spectrophotometer; and A4, calculating the content of the water-soluble calcium. The method for detecting the content of the water-soluble calcium in the dried oysters by using a calcein fluorescent probe is established, and when the method is used for detecting the content of the water-soluble calcium in the dried oysters, the consumed time is short, the detection data is accurate, and the stability is high.

Description

technical field [0001] The invention belongs to the technical field of inspection, and in particular relates to a method for detecting water-soluble calcium content in dried oysters. Background technique [0002] Oyster is an important and common marine shell crop in my country. Studies have found that the protein content of oyster dried meat is 43% to 50%, the fat content is 5% to 10%, and the total sugar is 20% to 39%. In addition, The types and contents of vitamins are also quite rich, and the iodine content is much higher than that of dairy products. The minerals contained in oysters are also far stronger in quantity and quality than squid, sea cucumbers, clams and other similar types of seafood, and the calcium content is 40-94.04mg / g. [0003] The calcium in oysters belongs to the biological calcium in the inorganic calcium source. This type of calcium can be transformed into calcium that is easier for the human body to absorb and absorb through some unique processing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N21/64
Inventor 李达谅陈冬婷张瑶林泽榕邓威力
Owner FUJIAN NORMAL UNIV
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