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PDRN-containing whitening and freckle-removing composition and application thereof

A whitening and freckle-removing composition technology, which is applied in the field of whitening and freckle-removing compositions, can solve the problems of reducing the transmission speed of melanosomes, repeated whitening and freckle-removing effects, and inconspicuous whitening and freckle-removing effects, and achieves the repair of epidermal defense function and stable curative effect , promote skin collagen synthesis effect

Pending Publication Date: 2021-04-16
王超云
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main components of whitening and freckle removal currently on the market are divided into three categories: 1. Substances that inhibit tyrosinase activity and reduce melanin production, mainly including vitamin C and its derivatives, arbutin and its derivatives, kojic acid and its derivatives. 2. Substances that reduce the transmission speed of melanosomes to keratinocytes and reduce the melanin content of each epidermal cell layer mainly include azelaic acid, nicotinamide, etc.; 3. Promote epidermal metabolism and make melanin small Substances that shed quickly from the body mainly include fruit acid, keratinase, etc. Although the above substances are safe, their transdermal absorption effect is poor, resulting in insignificant whitening and freckle removal effects; their whitening and freckle removal effects are easy to repeat after interval stop use; increase the dose Although it can improve the effect of whitening and freckle removal to a certain extent, excessive dosage can easily cause problems such as skin intolerance

Method used

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  • PDRN-containing whitening and freckle-removing composition and application thereof
  • PDRN-containing whitening and freckle-removing composition and application thereof
  • PDRN-containing whitening and freckle-removing composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Prepare PDRN (polydeoxyribonucleotide), comprising the following steps:

[0044] (1) Mix male salmon testis with 1 wt% trichloroacetic acid solution in a mass ratio of 1:4, homogenate, centrifuge at 4000 rpm for 5 min, and discard the supernatant;

[0045] (2) Add lysate to the precipitate obtained in step (1), mix well, heat in a water bath at 90°C for 15min, cool to room temperature and centrifuge at 6000rpm for 10min, and take the supernatant; the lysate contains 100mM Tris-HCl , 200mM NaCl, 5mM EDTA and 0.2wt% SDS, the pH of which is 8.0;

[0046] (3) Add an equal volume of 4°C pre-cooled isopropanol to the supernatant obtained in step (2), mix well, place it at -20°C for 30 minutes, centrifuge at 8000 rpm for 10 minutes, discard the supernatant, collect the precipitate, and Air-dry the precipitate until there is no ethanol smell to obtain PDRN.

[0047] 2. Perform gel electrophoresis on the PDRN prepared above:

[0048] Accurately weigh PDRN, add TE solution,...

Embodiment 2

[0051] 1. Preparation of PDRN (polydeoxyribonucleotide)

[0052] (1) Mix male salmon testis with 1 wt% trichloroacetic acid solution at a mass ratio of 1:10, homogenate, centrifuge at 4000 rpm for 5 min, and discard the supernatant;

[0053] (2) Add lysate to the precipitate obtained in step (1), mix well, heat in a water bath at 95°C for 10 minutes, cool to room temperature and centrifuge at 7000 rpm for 10 minutes, and take the supernatant; the lysate contains 150mM Tris-HCl , 200mM NaCl, 4mM EDTA and 0.25wt% SDS, its pH is 8.2;

[0054] (3) Add an equal volume of 4°C pre-cooled absolute ethanol to the supernatant obtained in step (2), mix well, place it at -20°C for 30min, centrifuge at 8000rpm for 10min, discard the supernatant, collect the precipitate, and Air-dry the precipitate until there is no ethanol smell to obtain PDRN.

[0055] 2. Perform gel electrophoresis on the PDRN prepared above: use gel electrophoresis to measure the molecular weight of DNA in the sample,...

Embodiment 3

[0057] 1. Preparation of PDRN (polydeoxyribonucleotide)

[0058] (1) Mix male salmon ovary with 1 wt% trichloroacetic acid solution at a mass ratio of 1:4, homogenate, centrifuge at 4000 rpm for 5 min, and discard the supernatant;

[0059] (2) Add lysate to the precipitate obtained in step (1), mix well, heat in a water bath at 70°C for 20min, cool to room temperature and centrifuge at 7000rpm for 10min, and take the supernatant; the lysate contains 100mM Tris-HCl , 150mM NaCl, 2mM EDTA and 0.15wt% SDS, the pH of which is 8.0;

[0060] (3) Add an equal volume of 4°C pre-cooled absolute ethanol to the supernatant obtained in step (2), mix well, place it at -20°C for 30min, centrifuge at 8000rpm for 10min, discard the supernatant, collect the precipitate, and Air-dry the precipitate until there is no ethanol smell to obtain PDRN.

[0061] 2. Perform gel electrophoresis on the PDRN prepared above:

[0062] The molecular weight of DNA in the sample was determined by gel electro...

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Abstract

The invention discloses a PDRN-containing whitening and freckle-removing composition. The whitening and freckle-removing composition is prepared from PDRN, nonapeptide, pentapeptide, hyaluronic acid and nicotinamide. The PDRN-containing whitening and freckle-removing composition is prepared from the following components in parts by weight: 0.05 to 5 parts of PDRN, 0.05 to 0.5 part of nonapeptide, 0.001 to 0.5 part of pentapeptide and 0.1 to 7 parts of nicotinamide. The invention also discloses application of the whitening and freckle-removing composition in cosmetics. The composition and the cosmetic containing the composition have a synergistic effect, can inhibit the formation of melanin from multiple angles, activate the activity of aged cells, promote the synthesis of collagen and elastin, reconstruct and repair an epidermis defense function, improve a skin cell microenvironment, increase the water content of skin, improve the elasticity and smoothness of the skin, and reduce the generation of inflammatory factors. Therefore, the problems of dryness, roughness, looseness, fine lines, darkness and the like of the skin are solved in all angles.

Description

technical field [0001] The invention belongs to the field of cosmetics, and in particular relates to a PDRN-containing whitening and freckle-removing composition and an application thereof. Background technique [0002] Aging (Aging), also known as aging, is a natural process of life. The skin is located on the body surface and is the first tissue to show the aging of the body. With the increase of age, under the influence of endogenous and external environmental factors, the skin loses moisture, the melanocyte reaction increases, and the synthesis of collagen and elastin decreases, resulting in skin pigmentation, tissue relaxation, and increased wrinkles. Modern society is fiercely competitive and the pace of life is fast, which brings greater physical and psychological pressure to people. Coupled with environmental influences, it leads to various skin problems. Facial freckles, chloasma, age spots, and sagging skin affect women's appearance and self-confidence. Serious im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/60A61K8/64A61K8/67A61Q19/00A61Q19/02A61Q19/08C07H1/08C07H21/04
Inventor 王超云高原
Owner 王超云
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