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Application of product for detecting gas production rate of neonatal intestinal flora in preparation of product for predicting, diagnosing or assisting diagnosis of neonatal NEC

A technology for intestinal flora and auxiliary diagnosis, applied in the field of medical testing, can solve the problem that the measurement of intestinal gas production cannot accurately reveal the patient's physical condition

Inactive Publication Date: 2021-04-13
SUZHOU HALO BIO TECH +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] One aspect of the present invention is aimed at the lack of accurate markers in the prior art to predict, diagnose or assist in the diagnosis of neonatal NEC, and at the same time, the measurement of intestinal gas production in existing studies cannot accurately reveal the patient's physical condition The problem is to provide a new application of a product for detecting the gas production of neonatal intestinal flora in the preparation of products for predicting, diagnosing or assisting in the diagnosis of neonatal necrotizing enterocolitis

Method used

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  • Application of product for detecting gas production rate of neonatal intestinal flora in preparation of product for predicting, diagnosing or assisting diagnosis of neonatal NEC
  • Application of product for detecting gas production rate of neonatal intestinal flora in preparation of product for predicting, diagnosing or assisting diagnosis of neonatal NEC
  • Application of product for detecting gas production rate of neonatal intestinal flora in preparation of product for predicting, diagnosing or assisting diagnosis of neonatal NEC

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1: Collection and grouping of samples

[0058] 1. Fecal samples of premature infants were collected on the third day, the first week, the second week, the third week and the fourth week after the start of the experiment. After defecation, premature infants should change their diapers in time, scrape the feces on them with a sampling spoon, and deliver them to the laboratory as soon as possible to ensure that the inoculation is completed within 6 hours after the feces are discharged. Register sample information, record Bristol classification and stool color, and test stool pH.

[0059] 2. The number of samples was 50 (including non-control group samples from the experimental group were all converted to NEC). Divided into control group, LAT (lactose) group, 2fl (a kind of breast milk oligosaccharide) group, FOS (fructose oligosaccharide) group and GOS (galactooligosaccharide) group, 10 people in each group.

Embodiment 2

[0060] Example 2: Monitoring of gas production by intestinal flora in premature infants

[0061] 1. Weigh 0.8g±0.02g of fecal samples and put them into the feces treatment box, seal them and put them into the feces treatment apparatus, add diluent for processing, and make a 10% fecal suspension, and use a 1mL syringe with No. 5 Aspirate 0.5mL of the suspension on the clear side of the specimen box with a needle and puncture the butyl rubber stopper of the vial, and inject LAT (lactose), 2fl (a kind of breast milk oligosaccharide), FOS (fructose oligosaccharide) and GOS (oligosaccharide) respectively. galactose) medium. These prebiotics are most commonly found in formula / breastfeeding. Neonatal fecal samples were prepared at a final concentration of 1% in fermentation medium containing prebiotics and 0.8% final concentration of prebiotics.

[0062] The pressure was adjusted to atmospheric pressure with a nitrogen balloon, placed in an incubator at 37° C. for 24 hours, and tak...

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Abstract

The invention discloses application of a product for detecting a gas production rate of neonatal intestinal flora in preparation of a product for predicting, diagnosing or assisting diagnosis of neonatal NEC. The product for detecting the gas production rate of the neonatal intestinal flora further comprises prebiotics for fermenting excrement samples of newborns. According to the application of the product, the excrement suspension of a subject is directly inoculated into a culture medium for in-vitro fermentation, so that the gas components are detected, and the content of gas produced by microorganisms at the colon part of the subject, the composition components and the proportion of each component can be more accurately detected. Meanwhile, markers related to the necrotizing enterocolitis of newborns are found, and a new way is provided for prediction, diagnosis or auxiliary diagnosis of the newborn necrotizing enterocolitis.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to the use of a product for detecting the gas production of neonatal intestinal flora in preparing a product for predicting, diagnosing or assisting in diagnosing neonatal necrotizing enterocolitis. Background technique [0002] Neonatal necrotizing enterocolitis (NEC) is the most serious digestive system disease that endangers the health of neonates in the neonatal period, and is an important cause of death of neonates, especially premature infants. With the advancement of perinatal medical technology, the survival rate of premature infants and high-risk infants continues to improve, but the incidence of NEC is on the rise. 90% of NEC occurs in preterm infants, the incidence in live births is about 1-3 / 1000, NEC occurs in about 1-5% of all preterm infants admitted to the NICU, and 5-10% of these infants For very low birth weight infants, the mortality rate is as high as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3504G01N27/26G01N27/30G01D21/02C12Q1/04C12M1/34
CPCG01N21/3504G01N27/26G01N27/30G01D21/02C12M41/34C12Q1/04G01N2800/065
Inventor 杜昱朱立颖雷磊张克成陈洁莹李华文李降龙王欣
Owner SUZHOU HALO BIO TECH
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