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Method for determining content of garlicin content in garlicin tablets

A technology of allicin and a detection method, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of low allicin content, affecting the accuracy of content detection, affecting the dissolution of allicin, etc., and achieves a scientific, effective and stable detection method. Good, high recovery effect

Inactive Publication Date: 2017-04-26
BY HEALTH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these processing methods are not suitable for embedded allicin tablets, and all of them cannot dissolve and extract allicin well.
Because some excipients are added to the allicin tablets, such as α-dextrin, microcrystalline cellulose, sodium starch glycolate and other commonly used excipients in tablets will affect the dissolution of allicin, resulting in a very low content of allicin dissolved, affecting the subsequent content Accuracy of detection, unable to obtain accurate results of allicin content in the sample

Method used

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  • Method for determining content of garlicin content in garlicin tablets
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  • Method for determining content of garlicin content in garlicin tablets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Chromatographic conditions

[0039] Chromatographic column: Diamonsil Plus C18 (column length 250mm, inner diameter 4.6mm, particle size 5.0μm);

[0040] Column temperature: 30°C;

[0041] Flow rate: 1.0ml / min;

[0042] Wavelength: 240nm;

[0043] Mobile phase: acetonitrile: water = 70:30

[0044] 2. Preparation of reference substance solution: Accurately weigh 8 mg of allicin (diallyl trisulfide) reference substance, put it in a 25ml brown volumetric flask, add appropriate amount of N,N-dimethylformamide, and dissolve it by ultrasonication at 30°C for 15 minutes , add N,N-dimethylformyl to the volumetric flask mark, shake well, that is.

[0045] 3. Sample preparation for test solution: Take 20 pieces of allicin tablets to be tested (about 10g), grind them into powder, accurately weigh 50mg into a 25ml volumetric flask, add an appropriate amount of N,N-dimethylformamide in 30 Dissolve by ultrasonication at ℃ for 15 minutes, dilute the volume of N,N-dimethylforma...

Embodiment 2

[0093] The method of Example 1 and the routine detection methods (NY / T 1497-2007, NY / T 1800-2009 and NY / T 2643-2014) were used to detect diallyl trisulfide in the same batch of allicin tablets respectively (the content of diallyl trisulfide in known allicin tablets is 1.0%), the results are shown in Table 5.

[0094] Table 5 Different methods detect the content of diallyl trisulfide in allicin tablets

[0095] Detection method The content of diallyl trisulfide in allicin (%) NY / T 1497-2007 not detected NY / T 1800-2009 0.68％ NY / T 2643-2014 0.05％ Example 1 0.96％

Embodiment 3

[0097] 1. Chromatographic conditions

[0098] Chromatographic column: Diamonsil Plus C18 (column length 250mm, inner diameter 4.6mm, particle size 5.0μm);

[0099] Column temperature: 40°C;

[0100] Flow rate: 0.5ml / min;

[0101] Wavelength: 240nm;

[0102] Mobile phase: acetonitrile: water = 80:20

[0103] 2. Preparation of reference substance solution: Accurately weigh 5 mg of allicin (diallyl trisulfide) reference substance, put it in a 10ml brown volumetric flask, add appropriate amount of N,N-dimethylformamide, and sonicate at 4°C for 30 minutes Dissolve, add N,N-dimethylformyl to the volumetric flask mark, shake well, that is.

[0104] 3. Preparation of sample test solution: Take 20 allicin tablets to be tested (about 10g), grind them into powder, accurately weigh 30mg into a 10ml volumetric flask, add appropriate amount of N,N-dimethylformamide to Dissolve by ultrasonication at 4°C for 30 minutes, dilute the volume of N,N-dimethylformamide to the scale of the volum...

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Abstract

The invention belongs to the technical field of quality control, and particularly relates to a method for determining the content of garlicin diallyl trisulfide in garlicin tablets. The method comprises the steps as follows: a to-be-detected sample of the garlicin tablets is mixed and dissolved with N, N-dimethylformamide, detection with high performance liquid chromatography and analysis with an external standard method are performed, and the content of diallyl trisulfide is obtained. With the adoption of the detection method, diallyl trisulfide can be fully separated from the garlicin tablets, and accordingly, the content of diallyl trisulfide in the garlicin tablets can be accurately detected. The detection method has the advantages of good linearity, repeatability and stability, high precision, low detection limit and quantification limit and high recovery rate, and blanks basically have no interference to determined results.

Description

technical field [0001] The invention belongs to the technical field of quality control, in particular to a method for measuring the content of allicin in allicin tablets, in particular to a method for measuring the content of allicin diallyl trisulfide in allicin tablets. Background technique [0002] Allicin (garlicin), also known as allicin, is a diallyl thioether compound, the main components of which are diallyl trisulfide and diallyl disulfide. Allicin naturally exists in the bulbs of garlic, a plant of the Liliaceae family, and has antibacterial effects on a variety of Gram-positive and Gram-negative bacteria. Candida albicans, Cryptococcus, Aspergillus fumigatus), viruses (cytomegalovirus), amoeba, Trichomonas vaginalis, pinworms, etc. all have inhibitory and killing effects, especially for intestinal bacteria such as Escherichia coli and Shigella powerful. Clinical studies have found that allicin has a significant preventive effect on bone marrow transplant recipie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 叶少文苏昭仑游景水
Owner BY HEALTH CO LTD
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