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Streptomyces sulforabies and its application in the control of citrus green mildew

A technology of Streptomyces thioluteus and green mold, applied in the field of microorganisms, can solve the problems of single disease control effect not reaching the desired effect, waste of social resources, material and financial resources, poor biological control, etc.

Active Publication Date: 2022-07-01
GUANGXI UNIV FOR NATITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on microbial control of diseases has achieved good results, but the commonly used antagonistic strains of diseases and insect pests are mainly broad-spectrum, and rarely involve in-depth research on a single disease, and the control effect on a single crop and a single disease cannot achieve the ideal effect , resulting in a waste of social resources and material and financial resources, and have a negative impact on the promotion of biological control

Method used

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  • Streptomyces sulforabies and its application in the control of citrus green mildew
  • Streptomyces sulforabies and its application in the control of citrus green mildew
  • Streptomyces sulforabies and its application in the control of citrus green mildew

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Experimental program
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Embodiment Construction

[0030] 1. Screening of strains

[0031] 1.1 Material preparation

[0032] Sample sampling: The soil sampling site is the National Nature Reserve of Nonggang, Guangxi. The topsoil is removed, and the soil samples at a depth of 15 cm below the surface are collected, stored in a 4°C incubator, and brought back to the laboratory for further processing.

[0033] Separation and purification medium:

[0034] LB liquid medium: tryptone 10.0g, yeast powder 5.0g, sodium chloride 5.0g, distilled water 1000mL.

[0035] LB solid medium: tryptone 10.0g, yeast powder 5.0g, sodium chloride 5.0g, agar powder 15g, distilled water 1000mL.

[0036] PDA medium: potato 200.0 g, glucose 20.0 g, agar 20.0 g, distilled water 1000 mL.

[0037] 1.2 Isolation, purification and screening of strains

[0038] 1.2.1 Separation and purification

[0039] Add 2g of soil samples to 18ml of sterile water to prepare a soil suspension, shake it on a shaker (180r / min) for 30min, and dilute it to 10 with sterile...

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Abstract

The invention discloses Streptomyces thioluteus, the preservation number is GDMCC NO: 61327, and the classification name is Streptomycesthioluteus GXMD-hs-43. The strain is simple to screen and easy to cultivate, and the inventor has also established a corresponding cultivation method. Studies have shown that the fermentation broth of GXMD‑hs‑43 strain has a good control effect on citrus green mold, and its inhibitory effect on Penicillium digitorum is as high as 94.81%. In addition, the toxicological experiments of the GXMD‑hs‑43 strain showed that it is a non-toxic microorganism, so it also has the characteristics of good ecological safety, and will not cause secondary pollution during use. The discovery of this strain has enriched the available microbial resources in my country, and its bacteriostatic effect is stable, efficient and environmentally friendly, and it has a good application prospect in the prevention and treatment of citrus green mildew.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Streptomyces sulforabies and its application in preventing and treating citrus green mildew. Background technique [0002] Citrus green mold is caused by Penicillium digitatum. Citrus green mold can infect citrus through various wounds on citrus. Citrus green mold can survive on various organic substances. When the citrus epidermis has a wound, the conidia of the citrus green mold in the air will attach to the citrus wound and start to infect the fruit. Citrus green mold is a common storage disease of citrus, which often causes citrus fruit to rot and cause serious losses. [0003] At present, there are three main measures used to control citrus diseases after picking: physical control, chemical control and biological control. [0004] Chemical control is the most commonly used method to control citrus green mildew at this stage. Chemical control is the use o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/465
CPCC12N1/20A23B7/155
Inventor 朱英芝姜明国王一兵黄文善卢杰刘艳黄群
Owner GUANGXI UNIV FOR NATITIES
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