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A method for detecting the components of an oral liquid for clearing away heat, nourishing yin, promoting blood circulation and removing blood stasis

A technology of promoting blood circulation and removing blood stasis, oral liquid, which is applied in the field of analytical chemistry, can solve the problems of long detection time and easy deviation of measurement results, and achieve the effect of reducing test pressure, shortening test time, and good durability

Active Publication Date: 2022-04-08
JINLING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the actual inspection process, considering factors such as simultaneous detection of multiple batches and sample stability, there are situations where the detection time is too long and the measurement results are prone to deviations.

Method used

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  • A method for detecting the components of an oral liquid for clearing away heat, nourishing yin, promoting blood circulation and removing blood stasis
  • A method for detecting the components of an oral liquid for clearing away heat, nourishing yin, promoting blood circulation and removing blood stasis
  • A method for detecting the components of an oral liquid for clearing away heat, nourishing yin, promoting blood circulation and removing blood stasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of reference substance solution: refer to the preparation method of reference substance solution in the current quality standards of Mailuoning Injection and Mailuoning Oral Liquid, take appropriate amount of cinnamic acid reference substance and chlorogenic acid reference substance, accurately weigh, add 50% methanol to dissolve and dilute, A mixed solution containing 60 μg of chlorogenic acid and 2 μg of cinnamic acid per 1 mL was prepared.

[0033] Preparation of the test solution: Precisely measure 1 mL of Mailuoning Oral Liquid, put it in a 25 mL volumetric flask, add water to the mark, shake well, filter, and take the continuous filtrate.

[0034] Determination method: Precisely draw 2 μL of the test solution and the reference solution, respectively, and inject them into a high-performance liquid chromatograph. Chromatographic conditions: The chromatographic column is YMC Meteoric Core C18 (100mm×3.0mm, 2.7μm, 8nm), and the column temperature is 35°C ;...

Embodiment 2

[0036] The investigation of embodiment 2 chromatographic column

[0037] The preparation of the reference solution and the test solution is the same as that in Example 1.

[0038] (1), Kromasil C18 (250mm×4.6mm, 5μm) chromatographic column

[0039] Referring to the content determination method of cinnamic acid in Scrophulariaceae under the quality standard content determination of original Mailuoning injection, a Kromasil C18 (250mm×4.6mm, 5μm) chromatographic column was used, with methanol as mobile phase A and 0.1% phosphoric acid as mobile phase B, flow rate 1.0 mL / min, gradient elution was performed using the following gradient elution procedure (Table 1).

[0040] Table 1. Examination of gradient elution procedures

[0041]

[0042] Depend on Figure 4 It can be seen that the peak separation effect is good, but the sample detection time is more than 60 minutes, which is inconsistent with the purpose of shortening the inspection time of the present invention and increa...

Embodiment 3

[0055] Example 3 Gradient investigation

[0056] The preparation of the reference solution and the test solution is the same as that in Example 1. Except for adjusting the gradient elution procedure (see Table 2), the rest are the same as the assay method in Example 1.

[0057] Table 2. Examination of gradient elution procedures

[0058]

[0059] Depend on Figure 8 It can be seen that after adjusting the gradient elution program, the peak time can be shortened, but because the gradient transition is fast, the baseline separation between peaks cannot be completely achieved.

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Abstract

The invention discloses a method for determining the content of an oral liquid for clearing away heat, nourishing yin, promoting blood circulation and removing blood stasis, comprising: taking the oral liquid for testing and standard solution for HPLC detection, chromatographic conditions: the chromatographic column is YMC Meteoric Core C18 (100×3.0mm , 2.7μm, 8nm), column temperature 25~40℃; methanol as mobile phase A, acid aqueous solution as mobile phase B, gradient elution; diode array detector, chlorogenic acid detection wavelength is 327nm, cinnamic acid detection wavelength is 278nm; record the chromatogram, and calculate the content of chlorogenic acid and cinnamic acid in Mailuoning Oral Liquid by external standard method. The method of the invention greatly shortens the inspection time, is simple, feasible, fast and accurate, reduces inspection pressure, has good precision, linear relationship, stability, repeatability, high recovery rate, and good durability, and can be used as an evaluation method for the quality of Mailuoning oral liquid effective method.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a method for detecting components of an oral liquid for clearing heat, nourishing yin, promoting blood circulation and removing blood stasis. Background technique [0002] Mailuoning oral liquid is made of 5 medicinal materials such as honeysuckle, mountain silver flower, dendrobium, Achyranthes, and Scrophulariaceae. For thromboangiitis obliterans, arteriosclerotic obliterans, cerebral thrombosis and sequelae, venous thrombosis and other diseases. Pharmacological studies have shown that Mailuoning Oral Liquid has the functions of protecting the heart and cerebrovascular tissues, resisting thrombosis, improving microcirculation and improving blood rheology. [0003] The pharmaceutical research of Mailuoning Oral Liquid is relatively weak, and the content determination in the current standard only detects the index component cinnamic acid in Scrophulariaceae. The dosage form ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/60
CPCG01N30/6052
Inventor 张蕙夏云崔凌云汤茜李剑徐向阳周红燕田丽娟杨俊刘文一蒋舒瑶倪洁
Owner JINLING PHARMA
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