Recombinant adeno-associated virus transfer vector containing gD protein gene of variant porcine pseudorabies virus, virus and preparation and application thereof

Abstract

The invention belongs to the technical field of gene engineering, and particularly relates to a recombinant adeno-associated virus transfer vector containing a gD protein gene of a variant porcine pseudorabies virus, a recombinant adeno-associated virus, and preparation and application thereof. The recombinant adeno-associated virus (rAAV) is used as a carrier to express the porcine pseudorabies virus gD protein, and the rAAV has the advantages of safety, effectiveness, specificity and the like. The genetically engineered strain prepared by the method expresses the gD immunogenic protein of the epidemic variation porcine pseudorabies virus strain, and has good immunoprotection for multiple epidemic variation porcine pseudorabies virus strains. The mouse is subjected to intramuscular injection of the anterior shin of the right lower limb, then the body can be continuously induced to generate specific neutralizing antibodies and cellular immunity at a high level.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and more specifically relates to a recombinant adeno-associated virus transfer vector containing a mutant porcine pseudorabies virus gD protein gene, a recombinant adeno-associated virus, and its preparation and application. Background technique [0002] Pseudorabies (PR) is caused by pseudorabies virus (PRV). The main symptoms of domestic animals (pigs, cattle, sheep) and wild animals are fever, itching and encephalitis. Since the clinical symptoms are similar to rabies, Therefore, it is also called pseudorabies. PRV has a wide range of hosts in nature, and pigs are the main natural and storage hosts. PRV can infect pigs of different ages and cause different clinical symptoms, especially the most serious damage caused by infection of pregnant sows and suckling piglets. PRV causes reproductive disorders in pregnant sows, neurological symptoms and high mortality in suckling piglets, ...

AI Technical Summary

Problems solved by technology

Classical vaccines have poor protection against mutant strains. It is necessary to use the clinically isolated mutant pseudorabies virus as a gene sample to develop a more effective vaccine to prevent and control pseudorabies. However, the whole virus is used to prepare inactivated vaccines and gene-deleted attenuated vaccines. It is cumbersome and costly, so DNA vaccines, subunit vaccines and live vector vaccines will be the main directions for the development of PRV vaccines

[0006] The commercial vaccine used as a control in this experiment is the porcine pseudorabies inactivated vaccine commercialized by Keqian Biotechnology Company. This commercial vaccine plays an important role in the prevention and control of PR. The loss of antigenic determinants leads to poor immune efficacy, requiring a large vaccination dose and multiple immunizations to achieve a good immune effect, and the components of inactivated vaccines are complex, and allergic reactions often occur

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