Method for separating and extracting chain ester compounds from Nigella sativa seeds

A technology for ester compounds and extracts, which is applied in the field of) and achieves the effects of simple extraction process, recyclable solvent and large extraction amount

Active Publication Date: 2021-02-02
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there is no information on the extraction and isolation of chain esters (such as 9-decenoic acid-12-hydroxyethyl ester, 9-decenoate, etc.) from Nigella sativa seeds. Monoenoic acid-1-(3-pentoxysilyl)-ethyl ester, etc.) method

Method used

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  • Method for separating and extracting chain ester compounds from Nigella sativa seeds
  • Method for separating and extracting chain ester compounds from Nigella sativa seeds
  • Method for separating and extracting chain ester compounds from Nigella sativa seeds

Examples

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Embodiment 1

[0031] A chain ester compound (9-decenoic acid-12-hydroxyethyl ester, 9-undecenoic acid-1-(3-pentoxysilyl)-ethyl ester) isolated from Nigella sativa seeds method, which specifically includes the following steps:

[0032] 1) Use 5kg of dry Nigella sativa seeds as raw material, soak and degrease with 7L petroleum ether at room temperature after crushing (defatting 3 times, each degreasing time is 3 days), evaporate the residue to dry petroleum ether, cool with 70% ethanol at room temperature Leach and extract 3 times (the ratio of residue to 70% ethanol to liquid is 5kg:7L), each time for 3 days, combine the extracts, recover the solvent under reduced pressure, and obtain the extract extract;

[0033] 2) Dissolve the extract extract in methanol, mix the sample on a silica gel column, and use dichloromethane- Gradient elution was performed on the silica gel column with methanol, and combined by silica gel thin-layer chromatography to obtain 7 components, which were labeled as Fr...

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Abstract

The invention relates to a method for separating and extracting chain ester compounds from Nigella sativa seeds, which comprises the steps of 1) pulverizing the dried Nigella sativa seeds, degreasingwith petroleum ether, extracting with 60-80% ethanol, and recovering the solvent under reduced pressure to obtain an extract; 2) dissolving the extract with methanol, mixing the extract with a silicagel column, and carrying out gradient elution with dichloromethane-methanol to obtain seven components Fr1-Fr7; 3) performing normal pressure silica gel column chromatography on the component Fr1 to obtain six components Fr1-1 to Fr1-6; and 4) performing gel column chromatography and silica gel column chromatography separation on the component Fr1-4 to obtain 9-decenoic acid-12-hydroxy ethyl ester, and performing gel column chromatography, silica gel column chromatography and reversed phase chromatography separation on the component Fr1-2 to obtain 9-undecylenic acid-1-(3-pentyloxy silane)-ethyl ester. The method has the advantages of simple extraction process, accessible product, recyclable solvent and large extraction amount, and can implement industrial production.

Description

technical field [0001] The invention belongs to the technical field of plant extraction and separation, in particular to a method for effectively and rapidly separating and extracting chain ester compounds (such as 9-decenoic acid-12-hydroxyethyl ester, 9-undecenoic acid, etc.) from Nigella sativa seeds. -1-(3-pentyloxysilyl)-ethyl ester, etc.) method. Background technique [0002] Nigella sativa seeds are the dried seeds of Nigella sativa, a plant of the Ranunculaceae Nigella genus. It is native to Southwest Asia and widely distributed in the Mediterranean, Central Europe, West Asia and other places; its seed oil is used as edible oil in the Middle East and North African countries such as It is widely used in Egypt, and at the same time, it is also used in health food to fight diseases, and it is widely used in food. The main components of Nigellasativa seeds are flavonoids, saponins, alkaloids, steroids and phenols, etc., and also contain a lot of oil. Nigella sativa see...

Claims

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Application Information

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IPC IPC(8): C07C67/56C07C69/732C07D303/42C07D301/32
CPCC07C67/56C07D303/42C07D301/32C07C69/732
Inventor 刘振花康文艺张岩马常阳牛云王莉屈娇娇王贵生
Owner HENAN UNIVERSITY
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