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Inverse opal structure microsphere with multiple molecular imprinting and preparation method and application thereof

An anti-opal structure and molecular imprinting technology, applied in ion exchange, ion exchange regeneration, ion exchange treatment devices, etc., can solve the problems of poor specificity of adsorbent, poor specific adsorption, and low adsorption efficiency, and achieve low cost and easy Simple effect of control and process

Active Publication Date: 2021-01-15
NANJING DRUM TOWER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the poor specificity of the existing adsorbents, other biomolecules will be adsorbed at the same time during the adsorption process, which will lead to the destruction of blood electrolyte balance and body fluid acid-base balance, making patients bear a greater burden in the process of blood purification treatment. risk
[0003] At present, the problem of low adsorption efficiency and poor specific adsorption is mainly solved by developing new high-efficiency adsorbents.

Method used

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  • Inverse opal structure microsphere with multiple molecular imprinting and preparation method and application thereof
  • Inverse opal structure microsphere with multiple molecular imprinting and preparation method and application thereof
  • Inverse opal structure microsphere with multiple molecular imprinting and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] An inverse opal microsphere with multiple molecular imprints, prepared by the following method:

[0028] Step S1, using silica nanoparticles to self-assemble into a silica photonic crystal sphere template;

[0029] Step S2, grafting surface imprinted molecules on the surface of the silica photonic crystal ball template by electrostatic adsorption to form surface molecular imprints;

[0030] Step S3, dissolving the internally imprinted molecule and the photoinitiator in the hydrogel prepolymerization solution, and then infiltrating the hydrogel prepolymerization solution containing the internally imprinted molecule and the photoinitiator into the silica photonic crystal sphere template, and curing by ultraviolet light Form a photonic crystal-hydrogel microsphere hybrid; wherein, the bioimprinting molecules include surface imprinting molecules and internal imprinting molecules to form their own spatial structures in the cured hydrogel; the surface imprinting molecules are...

Embodiment 2

[0033] An inverse opal microsphere with multiple molecular imprints, prepared by the following method:

[0034] Step S1, using silica nanoparticles with a particle size of 250nm to prepare SiO by droplet self-assembly method 2 Photonic crystal ball template;

[0035] Step S2. According to electrostatic adsorption, positively charged lysozyme molecules are grafted on the negatively charged SiO2 as surface imprinted molecules. 2 The surface of the photonic crystal ball template forms surface molecular imprints;

[0036] Step S3, urea and creatinine are dissolved in the hydrogel prepolymerization solution as internal imprinted molecules and photoinitiator, the mixing ratio is 20% (v / v) gelatin methacrylate (GelMA), 20% (v / v) Polyethylene glycol diacrylate (PEGDA), 5% (w / v) urea, 5% (w / v) creatinine, and 1% (v / v) photoinitiator, prepolymerized the above mixed hydrogel The solution penetrates into the SiO 2 In the photonic crystal ball template, after soaking for 4 hours, it is...

Embodiment 3

[0038] Example 3: Inverse Opal Structured Microspheres with Multiple Molecular Imprints for Adsorption and Clearance of Blood Molecules

[0039] Taking the inverse opal microspheres with multiple molecular imprints of Example 2 as an example to verify the specific adsorption of target biomolecules in blood, the steps are as follows:

[0040] (1) Solution preparation:

[0041]Prepare aqueous solutions of urea, creatinine and lysozyme with concentrations of 1 mg / ml, 800 μg / ml, 600 μg / ml, 400 μg / ml, 200 μg / ml, and 100 μg / ml respectively, wherein the concentrations of the three target molecules in the aqueous solution of each concentration refer to Replace the concentration of urea, creatinine and lysozyme, such as the concentration of urea, creatinine and lysozyme in the aqueous solution of 1mg / ml urea, creatinine and lysozyme is 1mg / ml;

[0042] Prepare fetal bovine serum solutions of urea, creatinine, and lysozyme at concentrations of 1 mg / ml, 800 μg / ml, 600 μg / ml, 400 μg / ml, ...

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Abstract

The invention discloses an inverse opal structure microsphere with multiple molecular imprinting and a preparation method and application thereof. According to the method, a silicon dioxide photonic crystal sphere template is prepared through a liquid drop self-assembly method, surface imprinted molecules are grafted on the surface of the silicon dioxide photonic crystal sphere template through electrostatic adsorption, the silicon dioxide photonic crystal microsphere grafted with the surface imprinted molecules serves as the template, the hydrogel solution containing the internal imprinted molecules fills the internal gaps of the template, the template and the biological imprinted molecules are removed after ultraviolet curing to obtain the inverse opal structure microspheres with multiple molecular imprinting, the process is simple, and the cost is low; according to the inverse opal structure microsphere with multiple molecular imprinting prepared by the method, efficient and specific adsorption of target imprinting molecules can be realized through multiple molecular imprinting, and the inverse opal structure microsphere provides a reflection peak and a structural color with a specific wavelength, therefore, target biomolecules in blood can be efficiently and specifically adsorbed, the adsorption process can be monitored in real time, and the method can be applied to preparation of disease detection drugs.

Description

technical field [0001] The invention belongs to the technical field of adsorption materials, and in particular relates to an inverse opal microsphere with multiple molecular imprints, a preparation method and application thereof. Background technique [0002] Kidney disease is a disease with high morbidity and high fatality rate, including acute nephritis, chronic renal failure and so on. At present, hemodialysis, hemoperfusion and other treatment methods have been widely used in the treatment of kidney diseases. The principle is to remove excess metabolic wastes such as urea and creatinine in the patient's blood in vitro through adsorbents. Due to the poor specificity of the existing adsorbents, other biomolecules will be adsorbed at the same time during the adsorption process, which will lead to the destruction of blood electrolyte balance and body fluid acid-base balance, making patients bear a greater burden in the process of blood purification treatment. risk. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F299/00C08F299/02C08J9/28B01D15/08G01N21/25C08L55/00
CPCC08F299/00C08F299/028C08J9/28B01D15/08G01N21/25C08J2355/00Y02A50/30
Inventor 赵远锦陈涵旭王月桐池俊杰张大淦
Owner NANJING DRUM TOWER HOSPITAL
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