Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of acellular matrix and cyclopentenone prostaglandin composition

An acellular matrix, cyclopentenone technology, applied in tissue regeneration, medical science, prosthesis and other directions, to achieve the effect of good application prospects, good product consistency, and simple equipment requirements

Inactive Publication Date: 2021-01-08
SECOND AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] According to the literature search, acellular matrix combined with 15d-PGJ is currently used 2 -NC composition for repairing periodontal defects has not been reported yet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of acellular matrix and cyclopentenone prostaglandin composition
  • Preparation method of acellular matrix and cyclopentenone prostaglandin composition
  • Preparation method of acellular matrix and cyclopentenone prostaglandin composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 A kind of decellularized matrix and 15d-PGJ 2 -Preparation method of NC composition

[0035] 1. Preparation of acellular matrix:

[0036] (1) The periodontal ligament cells were seeded in a 12-well cell culture plate at a high density; the seeding density of the periodontal ligament cells was 1×10 5 pcs / hole.

[0037] (2) Add a complete medium containing a certain concentration of ascorbic acid to the culture plate to obtain periodontal ligament cell membranes; the concentration of ascorbic acid in the added medium is 100 μg / mL, and the culture period of the cell membranes is about 10 to 14 days. When the edge of the cell membrane is slightly curled, it means that the cell membrane has been matured.

[0038] (3) The PCL / GE electrospun nanofiber membrane was obtained by electrospinning, and the upper periodontal ligament cell membrane was wrapped after ultraviolet disinfection; 0.6g of PCL was dissolved in 10mL of tetrafluoroethylene (Tetrafluoroethylene, TF...

Embodiment 2

[0046] Embodiment 2 composition uses

[0047] An acellular matrix with 15d-PGJ 2 -The method that NC composition is used for periodontal defect restoration, comprises the following steps:

[0048] 1. Rat periodontal defect model was prepared by implanter equipment; the rats were 8-week-old male SD rats, and the size of the prepared periodontal defect area was 2 mm × 4 mm (height × width), and the depth was to reach the root surface without It is advisable to damage the root of the tooth, about 1-1.5mm.

[0049] 2. Put the acellular matrix based on the periodontal ligament cell membrane into the periodontal defect;

[0050] 3. Place an appropriate amount of 15d-PGJ on the surface of the acellular matrix 2 - NC gelatin sponge; gelatin sponge loaded 15d-PGJ 2 - NC concentration is 100 mg / L, volume is 25 μL.

[0051] 4. Suture in layers to close the wound.

[0052] Figure 4 Shown in the example will be based on periodontal ligament cell membrane decellularized matrix and l...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of an acellular matrix and cyclopentenone prostaglandin composition. The acellular matrix and cyclopentenone prostaglandin composition is formed by combining a periodontal ligament cell acellular matrix loaded on a polycaprolactone / gelatin electrospun nanofiber membrane and 15d-PGJ2-NC, and the acellular matrix and the 15d-PGJ2-NC are combined for the first time and used for periodontal defect. Compared with the application of a single component, the composition has tissue regeneration and anti-inflammatory effects, and is suitable for a periodontitis environment. According to the preparation method, gelatin-modified PCL / GE is used as a decellularized membrane support for the first time, and compared with pure PCL or PCL / GE with other blending ratios, better effects of promoting cell adhesion and vascularization are achieved. The preparation method and equipment of the required material are simple in requirement and low in cost, and the obtained material is high in consistency. Animal experiment results show that the composition provided by the invention can achieve a good effect of repairing periodontal defects, and provides preliminaryscientific evidences for clinical application of the composition.

Description

technical field [0001] The invention belongs to the field of tissue engineering and regenerative medicine, and relates to a preparation method of an acellular matrix and a cyclopentenone prostaglandin composition, which is an acellular matrix composed of periodontal ligament cell membranes and 15d-PGJ 2 -The composition preparation method of NC combination, which can be used for periodontal defect repair. Background technique [0002] Chronic periodontitis is a chronic infectious disease characterized by the destruction of periodontal tissue. The incidence rate in my country is as high as 80%, and it is the primary cause of tooth loss in adults. Periodontal tissue is composed of complex soft and hard tissues, including alveolar bone, periodontal ligament, and cementum. Repairing periodontal tissue defects is the ultimate goal of periodontal treatment. Traditional periodontal tissue regeneration therapy, such as bone grafting, guided tissue regeneration, growth factors, loca...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/36A61L27/54
CPCA61L27/3633A61L27/3641A61L27/3687A61L27/54A61L2430/12A61L2430/02A61L2300/22A61L2300/41A61L2300/412A61L2430/40
Inventor 吴燕岷柳佳美黄佳萍
Owner SECOND AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com