A kind of antifreeze production increasing composition that can be used for aerial protection
A composition and antifreeze technology, applied in the field of pesticides, can solve problems such as demulsification and flow
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Embodiment 1
[0056] Biological Assay Example 1: Determination of the synergistic effect of melatonin and pyraclostrobin on low temperature stress in wheat
[0057] Test object: the wheat variety is Yannong 19, purchased from the seed market;
[0058] Test method: The seeds were sterilized with 10% potassium permanganate liquid for 10 minutes, washed repeatedly with distilled water, and placed in a constant temperature incubator at 25° to accelerate germination. When the seeds are 95% white, they are sown in plastic cups equipped with vermiculite, and 10 seeds are sown in each plastic cup. It is grown under the culture environment conditions of temperature 20°, illuminance (PAR) 250umol / (m2.s), relative humidity 50%, and light 14h / d. Water the Hoagland nutrient solution with a pH of 5.5 every 4 days.
[0059]Quantitative melatonin was weighed, dissolved in absolute ethanol, and 1.5% Tween-80 was added to form a 2.5% solution. Quantitative pyraclostrobin was dissolved in acetone, and 1.5%...
Embodiment 2
[0074] Biological Assay Example 2: Determination of the synergistic effect of melatonin and thiamethoxam on low temperature stress in wheat
[0075] Test object: the wheat variety is Yannong 19, purchased from the seed market;
[0076] Test method: The seeds were sterilized with 10% potassium permanganate liquid for 10 minutes, washed repeatedly with distilled water, and placed in a constant temperature incubator at 25° to accelerate germination. When the seeds are 95% white, they are sown in plastic cups equipped with vermiculite, and 10 seeds are sown in each plastic cup. It is grown under the culture environment conditions of temperature 20°, illuminance (PAR) 250umol / (m2.s), relative humidity 50%, and light 14h / d. Water the Hoagland nutrient solution with a pH of 5.5 every 4 days.
[0077] Quantitative melatonin was weighed, dissolved in absolute ethanol, and 1.5% Tween-80 was added to form a 2.5% solution. Quantitative thiamethoxam was dissolved in methanol, and 1.5% T...
Embodiment 3
[0092] Biological Assay Example 3: Determination of the synergistic effect of pyraclostrobin and thiamethoxam on low temperature stress in wheat
[0093] Test object: the wheat variety is Yannong 19, purchased from the seed market;
[0094] Test method: The seeds were sterilized with 10% potassium permanganate liquid for 10 minutes, washed repeatedly with distilled water, and placed in a constant temperature incubator at 25° to accelerate germination. When the seeds are 95% white, they are sown in plastic cups equipped with vermiculite, and 10 seeds are sown in each plastic cup. It is grown under the culture environment conditions of temperature 20°, illuminance (PAR) 250umol / (m2.s), relative humidity 50%, and light 14h / d. Water the Hoagland nutrient solution with a pH of 5.5 every 4 days.
[0095] Quantitative pyraclostrobin was weighed, dissolved in acetone, and 1.5% Tween-80 was added to form a 2.5% solution. A quantitative amount of thiamethoxam was weighed and dissolve...
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