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Primary screening method for medicinal material quality

A technology for medicinal materials and quality, applied in measurement devices, analytical materials, material excitation analysis, etc., can solve the problems of high cost of liquid chromatography equipment, complex components of traditional Chinese medicine systems, low analysis and detection efficiency, etc., achieving excellent stability and low cost. , The effect of solution preparation is simple

Inactive Publication Date: 2020-12-29
ZHEJIANG UNIV
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  • Description
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Problems solved by technology

[0003] These index components are generally quantified by liquid chromatography, which has the advantage of accurate and reliable results, but there are also many shortcomings.
First, liquid chromatography not only has high equipment costs, but also has high requirements for the place where the chromatograph is placed and analysts, and the overall operating cost is relatively high
Second, the composition of some traditional Chinese medicine systems is complex, and the sample analysis time is long, which makes the analysis and detection efficiency not high

Method used

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  • Primary screening method for medicinal material quality
  • Primary screening method for medicinal material quality
  • Primary screening method for medicinal material quality

Examples

Experimental program
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Effect test

Embodiment 1

[0024] see figure 1 , respectively dissolving citric acid and ethylenediamine in a mixed solvent of water and tetraethylene glycol to prepare two solutions, and heating the two solutions to prepare carbon quantum dots after mixing. The properties of the carbon quantum dots were characterized, such as Figure 2-4 shown.

[0025] Honeysuckle is used as the medicinal material to be tested, and chlorogenic acid is used as the index component. Different concentrations of chlorogenic acid were contacted with carbon quantum dots, and the method of combining paper-based chips and smart phones was used to detect and determine the quenching of fluorescence, and the quantitative equation was obtained as Y=0.917+3.62X. The honeysuckle of the batch to be tested is taken, extracted with water to obtain an extract, and the extract is diluted to quench the fluorescence of the carbon quantum dots. After substituting the result of quenching the fluorescence of carbon quantum dots in the extr...

Embodiment 2

[0027] see figure 1 , respectively dissolving citric acid and ethylenediamine in a mixed solvent of water and tetraethylene glycol to prepare two solutions, and heating the two solutions to prepare carbon quantum dots after mixing. Salvia miltiorrhiza was used as the medicinal material to be tested, and salvianolic acid B was used as the index component. Different concentrations of salvianolic acid B were contacted with carbon quantum dots, and the method of combining paper-based chips and smart phones was used to detect and determine the fluorescence quenching, and the quantitative equation was obtained as Y=1.23+2.86X. The salvia miltiorrhiza of the batch to be tested is extracted with water to obtain an extract, which is diluted and used to quench the fluorescence of the carbon quantum dots. After substituting the result of quenching the fluorescence of carbon quantum dots in the extract into the quantitative equation, the total phenolic concentration in the salvianolic ac...

Embodiment 3

[0029] see figure 1 , dissolving citric acid and ethylenediamine in water, and preparing carbon quantum dots after microwave heating. Mulberry leaves were used as the medicinal materials to be tested, and rutin was used as the index component. The rutin solutions of different concentrations were contacted with the carbon quantum dots on a 96-well plate, and the fluorescence quenching was detected by a microplate reader, and the quantitative equation was obtained as Y=1.15+2.97X. The batch of mulberry leaves to be tested is extracted with water, and the obtained extract is diluted and used to quench the fluorescence of the carbon quantum dots. After substituting the result of quenching the fluorescence of carbon quantum dots in the extract into the quantitative equation, the total phenolic concentration in the mulberry leaf extract is converted to 0.17mg / mL equivalent to rutin, which is converted to a total phenolic value of 0.68% in the mulberry leaf medicinal material . Th...

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Abstract

The invention discloses a primary screening method for medicinal material quality. The method comprises the following steps of 1, preparing carbon quantum dots; 2, establishing a quantitative equationof the concentration of a reference substance and the fluorescence quenching condition of the carbon quantum dots by adopting the reference substance of an index component M of a medicinal material to be detected; 3, quenching the fluorescence of the carbon quantum dots by using a medicinal material extracting solution, and calculating a total phenol value in the medicinal material according to the fluorescence quenching condition; and 4, comparing the calculated total phenol value of the medicinal material with the standard of the index component, and judging that the quality of the medicinal material is unqualified if the total phenol value does not reach the lower limit of the standard. According to the method, the carbon quantum dots are used as a detection reagent, no toxic solvent is needed during detection, the solution preparation is simple, the detection time is short, the cost of detection equipment is relatively low, and the stability of long-term storage is obviously better. The method can be used for quickly judging the quality of medicinal materials taking phenolic components as index components.

Description

technical field [0001] The invention belongs to the technical field of medicine preparation, and in particular relates to a method for preliminary screening of medicinal material quality. Background technique [0002] The quality of most Chinese patent medicines is mainly affected by the quality of Chinese herbal medicines, so the quality control of Chinese herbal medicines is very important. There are many factors that affect the quality of Chinese herbal medicines. In addition to the place of origin and origin, it also includes various conditions in multiple links such as planting, harvesting, processing, storage and transportation. It is generally necessary to conduct relevant inspections on Chinese herbal medicines before use to determine whether they are qualified. In the Chinese Pharmacopoeia, in addition to using methods such as identification and inspection to judge the quality of medicinal materials, liquid chromatography is often used to quantitatively determine t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 程翼宇瞿海斌龚行楚
Owner ZHEJIANG UNIV
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