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Blood fat test card and application thereof

A technology of test card and blood lipid, which is applied in the field of blood lipid test card, can solve the problems that the components of the three items are not completely separated, the test results interfere with each other, and the anti-interference ability is poor, so as to improve the uniformity of detection, improve the convenience of operation, and improve the specific effect

Inactive Publication Date: 2020-12-04
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above detection method also has the problem of poor anti-interference ability, or the three item components in the structure are not completely separated, which will cause the three test results to interfere with each other as the sample flows, or due to the large amount of reduced substances contained in the sample such as ascorbic acid etc. react with the intermediate product hydrogen peroxide, resulting in a serious low test result

Method used

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  • Blood fat test card and application thereof
  • Blood fat test card and application thereof
  • Blood fat test card and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] This embodiment provides a method for preparing a blood lipid test card, which is prepared according to the following method.

[0041] (1) Preparation of diffusion layer:

[0042] Preparation of the diffusion layer treatment liquid: Measure 100 mL of purified water, add the following substances respectively and stir and mix evenly: 0.2 g Tween 20.

[0043] Diffusion layer treatment: Cut tea filter paper (Shanghai Kaining Paper: RF17) into 100 mm×200 mm, soak in the above treatment solution for 5 minutes, take it out, drain the remaining liquid, and place it horizontally in an oven at 45°C to dry for 1 hour, take it out and set aside.

[0044] (2) Preparation of HDL filter layer

[0045] Preparation of HDL precipitant treatment solution: Measure 100mL of purified water, add the following substances respectively and stir and mix evenly: 1.0g dextran sulfate 2000.

[0046] Treatment of HDL blood filter layer: Cut the blood filter membrane (Shanghai Jieyi Biological XQ-Y...

Embodiment 2

[0060] This embodiment provides a method for preparing a blood lipid test card, which is prepared according to the following method.

[0061] (1) Preparation of diffusion layer:

[0062] Preparation of the diffusion layer treatment liquid: Measure 100 mL of purified water, add the following substances respectively and stir and mix evenly: 0.2 g Tween 20.

[0063] Diffusion layer treatment: Cut tea filter paper (Shanghai Kaining Paper: RF17) into 100 mm×200 mm, soak in the above treatment solution for 5 minutes, take it out, drain the remaining liquid, and place it horizontally in an oven at 45°C to dry for 1 hour, take it out and set aside.

[0064] (2) Preparation of HDL filter layer

[0065] Preparation of HDL precipitant treatment solution: Measure 100mL of purified water, add the following substances respectively and stir and mix evenly: 1.0g dextran sulfate 10000.

[0066] Treatment of HDL blood filter layer: Cut the blood filter membrane (Shanghai Jieyi Biological XQ-...

Embodiment 3

[0080] Anti-interference evaluation of blood lipid test card.

[0081] Different concentrations of ascorbic acid were added to the blood samples at a ratio of 1 / 20 to obtain samples containing series of concentrations of ascorbic acid, which were tested by the blood lipid test card in Example 1 and Example 2. The results are shown in Table 1 and Table 2.

[0082]

[0083]

[0084] The interference rate of 200mg / L ascorbic acid on the blood lipid test card of the present invention is within 10%, showing good anti-interference ability.

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Abstract

The present invention discloses a blood fat three-item test card. The blood fat three-item test card comprises an upper cover (100), blood fat detection test paper (200) and a base seat (300); a sample adding hole (101) is formed in the upper cover (100); three detection holes are formed in the base seat (300), namely a first detection hole (301a), a second detection hole (301b) and a third detection hole (301c) corresponding to three modules of the blood fat detection test paper respectively; and the components of the blood fat detection test paper sequentially comprises a filter layer (201),a diffusion layer (202), a blood filter layer (203), an interception layer (204) and a reaction layer (205) from top to bottom. The blood fat test card is convenient to operate and high in anti-interference capability, and can detect total cholesterol, triglyceride and high-density lipoprotein cholesterol in human blood.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a blood lipid test card and its application. Background technique [0002] Blood lipids are the general term for neutral fats (triglycerides) and lipids (phospholipids, glycolipids, sterols, steroids) in plasma, which are widely present in the human body. The main components of blood lipids are triglycerides and cholesterol, of which triglycerides participate in energy metabolism in the human body, while cholesterol is mainly used for the synthesis of cell membranes, steroid hormones and bile acids. [0003] Plasma lipids are insoluble or slightly soluble in water, so lipids are transported in the blood circulation in the form of lipoprotein complexes with greater solubility. The protein part of lipoproteins is called apolipoprotein (Apo), and there are many types of apolipoproteins, generally divided into 5-7 categories. The protein part of high-density lipoprotein apoA1 i...

Claims

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Application Information

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IPC IPC(8): C12Q1/60C12Q1/61C12Q1/44C12Q1/26C12Q1/28C12Q1/37C12Q1/48C12M1/40C12M1/34C12M1/00
CPCC12Q1/60C12Q1/61C12Q1/44C12Q1/26C12Q1/28C12Q1/37C12Q1/485
Inventor 邹继华刘献文方亮贾江花薛杨波
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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