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Apatite microcarrier and preparation method and application thereof

An apatite and microcarrier technology, which can be applied in general culture methods, biochemical equipment and methods, chemical instruments and methods, etc. The effect of good stability and excellent biocompatibility

Pending Publication Date: 2020-12-04
浙江拜尔克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing microcarrier beads are usually large in diameter (typically 2-3mm), which creates difficulties in implantation and tissue integration, often leads to poor cell proliferation and reduced cell viability, and inevitably leads to poor tissue differentiation and repair Problems such as poor function
Furthermore, due to their low density, existing microcarrier beads cannot provide the protection of the stirred fluid environment required to achieve high cell yields in bioreactors

Method used

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  • Apatite microcarrier and preparation method and application thereof
  • Apatite microcarrier and preparation method and application thereof
  • Apatite microcarrier and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: the preparation of apatite microcarrier

[0048] A preparation method of apatite microcarrier, comprising the following steps:

[0049] (1) Add hydroxyapatite powder (synthesized by wet precipitation method) to solution A (solution A is mannuronic acid and guluronic acid dissolved in deionized water at a mass ratio of 1.56:1) , the concentration is 0.035g / mL), the mass of hydroxyapatite powder is 45% of the mass of solution A, then starch is added, the mass of starch is 40% of the mass of solution A, and stirred for 10min to obtain suspension B ;

[0050] (2) Extrude the suspension B prepared in step (1) dropwise through a droplet device (a droplet device controlled by an air-assisted electric valve) (the valve is opened at 6ms and closed at 90ms for extrusion) and added dropwise Concentration is in the calcium chloride solution of 0.5mol / L, forms pearl a, washes pearl a twice with deionized water then, then pearl a is placed in the isopropanol solution ...

Embodiment 2

[0054] Embodiment 2: the preparation of apatite microcarrier

[0055] A preparation method of apatite microcarrier, comprising the following steps:

[0056] (1) Add silicon ion-substituted hydroxyapatite powder (silicon ion-substituted hydroxyapatite powder synthesized by wet precipitation method) into solution A (solution A is calcium alginate dissolved in deionized water, the concentration is 0.03g / mL), the mass of hydroxyapatite powder replaced by silicon ions is 45% of the mass of solution A, then starch is added, the mass of starch is 40% of the mass of solution A, and stirred for 10min to obtain suspension B;

[0057] (2) Extrude the suspension B prepared in step (1) dropwise through a droplet device (a droplet device controlled by an air-assisted electric valve) (the valve is opened at 6ms and closed at 90ms for extrusion) and added dropwise Concentration is in the calcium chloride solution of 0.5mol / L, forms pearl a, washes pearl a twice with deionized water then, th...

Embodiment 3

[0058] Embodiment 3: the preparation of apatite microcarrier

[0059] A preparation method of apatite microcarrier, comprising the following steps:

[0060] (1) Add tricalcium phosphate powder (tricalcium phosphate powder synthesized by wet precipitation method) into solution A (solution A is calcium alginate dissolved in deionized water, concentration is 0.03g / mL) to disperse, the tricalcium phosphate powder The mass is 45% of the mass of solution A, then add amphene, the mass of camphene is 40% of the mass of solution A, stir for 10min, obtain suspension B;

[0061] (2) Extrude the suspension B prepared in step (1) dropwise through a droplet device (a droplet device controlled by an air-assisted electric valve) (the valve is opened at 6ms and closed at 90ms for extrusion) and added dropwise Concentration is in the calcium chloride solution of 0.5mol / L, forms pearl a, washes pearl a twice with deionized water then, then pearl a is placed in the isopropanol solution containin...

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Abstract

The invention belongs to the technical field of biological materials, and discloses an apatite microcarrier and a preparation method and application thereof. The heat-resistant temperature of the apatite microcarrier is 1000-1350 DEG C, the apatite microcarrier is mainly prepared from at least one of hydroxyapatite, halogen apatite, tricalcium phosphate, tetracalcium phosphate and apatite subjected to ion replacement. The invention also provides a preparation method of the apatite microcarrier. The apatite microcarrier still has good thermal stability at a high temperature of 1150 DEG C or above, and can be fully sterilized.The apatite microcarrier has no residual organic solvent, has no toxicity to cells, has good biocompatibility, and is beneficial to the application of the apatite microcarrier in the medical field.

Description

technical field [0001] The invention belongs to the technical field of biological materials, in particular to an apatite microcarrier and its preparation method and application. Background technique [0002] Compared with the traditional two-dimensional adherent cell culture technology, microcarriers are spherical beads that can support adherent cells in three-dimensional space for adhesion, proliferation and differentiation. Different from planar cell culture, microcarrier particles have a larger surface area / volume ratio, which effectively increases the area for cell adhesion and growth, thereby greatly increasing the number of cells that can be cultured in the same volume, which is very conducive to cell growth. scale cultivation. At the same time, because the microcarrier is easy to move and add, it can also reduce the number of passages in cell culture, effectively reducing the damage to the cells caused by the use of trypsin. Based on the multiple advantages of micro...

Claims

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Application Information

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IPC IPC(8): C01B25/32C12N5/00
CPCC01B25/327C12N5/0075C01P2004/60C01P2004/61C01P2004/62C01P2004/32C01P2004/03C01P2002/72C12N2513/00C12N2533/18
Inventor 杨智杰
Owner 浙江拜尔克生物科技有限公司
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