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Tumor diagnosis and treatment integrated nano material based on manganese base, preparation method and application

A nanomaterial, tumor technology, applied in antitumor drugs, photodynamic therapy, preparations for in vivo experiments, etc., can solve the problems of low R1, lack of targeting, short circulation time in vivo, etc. The effect of high biocompatibility and long circulation time in the body

Active Publication Date: 2020-10-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing small molecule CAs have disadvantages such as low R1, short circulation time in vivo, and lack of targeting.

Method used

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  • Tumor diagnosis and treatment integrated nano material based on manganese base, preparation method and application
  • Tumor diagnosis and treatment integrated nano material based on manganese base, preparation method and application
  • Tumor diagnosis and treatment integrated nano material based on manganese base, preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Preparation of nanomaterials

[0034] (1) Hypericin (Hyp) (0.61 mg) was dissolved in 200 μL of dimethyl sulfoxide (DMSO). Weigh manganese chloride (1.2 mg) and dissolve it in 200 μL deionized water. Bovine serum albumin (BSA) (400mg) was dissolved in 5.0mL deionized water, 1M NaOH was added dropwise into the BSA solution to adjust the pH value to 10, and then the Hyp and MnCl 2 The solution was slowly added dropwise to the BSA solution successively, followed by the dropwise addition of 1M NaOH to adjust the pH value of the mixture to 9.

[0035] (2) React at room temperature for 2 hours in the dark.

[0036] (3) The dialysis membrane (3500Da) was purified by dialysis in deionized water for 24 hours.

[0037] (4) freeze-drying to obtain brown powdery BHM nanoparticles.

[0038] 2. Performance analysis of nanomaterials

[0039] (1) Use a dynamic light scattering instrument (DLS) to detect the above-mentioned BHM nanoparticles, the results are as follows figure 1 ...

Embodiment 2

[0049] Nanomaterials were prepared according to the reaction conditions in Table 1, and the process flow was referred to in Example 1. The particle size of the nanomaterials prepared in Table 1 was shown.

[0050] Table 1

[0051]

[0052] It can be seen from Table 1 that when the mass ratio of bovine serum albumin, hypericin and manganese chloride is 400:0.61:1.2, the particle size of the obtained nanomaterials is the best. When the concentration of manganese chloride is constantly increasing, the particle size distribution of nanomaterials is constantly increasing, and too large nanoparticles cannot be enriched near the tumor tissue through the EPR effect, and are easily trapped by the reticuloendothelial system, making magnetic resonance imaging more difficult. And the effect of photodynamic therapy decreased.

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Abstract

The invention discloses a tumor diagnosis and treatment integrated nano material based on manganese base, a preparation method and application. According to the preparation method, albumin, hypericinand manganese chloride are added into an aqueous medium, and the product is self-assembled to form the tumor diagnosis and treatment integrated nano material. An efficient manganese-base nanoscale macromolecular contrast agent is prepared through self-assembly and has the advantages of being high in relaxation efficiency, long in in-vivo circulation time, rapid in kidney removal, high in targetingproperty and biocompatibility, small in toxic and side effect and the like. The nano material prepared by the invention has relatively high photodynamic conversion efficiency at 595nm, can be used asa photosensitizer to be applied to photodynamic therapy. In addition, the position and size of a tumor and the enrichment condition of the phototherapeutic agent in tumor tissue are monitored by means of a magnetic resonance technology, the nano material is used for evaluating the treatment effect, and magnetic resonance imaging-mediated photodynamic therapy diagnosis and treatment integration isachieved.

Description

technical field [0001] The invention relates to the field of integrated materials for diagnosis and treatment of photodynamic therapy mediated by magnetic resonance imaging, in particular to a manganese-based nanometer material for integration of diagnosis and treatment of tumors, a preparation method and an application. Background technique [0002] Photodynamic therapy (PDT) is a non-invasive, light-excited cancer treatment modality. The elements involved in PDT treatment are excitation light, photosensitizer and molecular oxygen. Under the excitation of a light source with a specific wavelength, photosensitizers can be selectively activated to generate ROS, followed by cytotoxicity, vascular damage and immune regulation, and finally induce cancer cell death. Photosensitizers are a necessary part of PDT. The common ones are porphyrin-related macrocyclic compounds, non-porphyrin macrocyclic compounds (such as phthalocyanine, porphine, hypericin and phenothiazines, etc.), a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/14A61K41/00A61P35/00
CPCA61K49/1869A61K49/143A61K41/0057A61P35/00
Inventor 胡红杰周晓璇唐建斌韩玉鑫
Owner ZHEJIANG UNIV
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