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Tumor cell vaccine and preparation method thereof

A tumor cell vaccine and tumor cell technology, applied in the field of immunotherapy, can solve the problem of weak immunogenicity of tumor cell vaccines, and achieve the effects of preventing and inhibiting tumor growth, preventing and inhibiting growth, and increasing immune response.

Pending Publication Date: 2020-09-11
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is: tumor cell vaccine immunogenicity is not strong

Method used

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  • Tumor cell vaccine and preparation method thereof
  • Tumor cell vaccine and preparation method thereof
  • Tumor cell vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of Multivalent Antibody-Combined Colon Cancer Tumor Cell Vaccine

[0046] 1) Inactivation: CT26 (colon cancer) tumor cells in the logarithmic growth phase are treated with X-ray irradiation (50Gy), so that they cannot proliferate but maintain a living state.

[0047] 2) Preparation of tumor cell antibody: immunize rabbits with radiation-inactivated tumor cells at a concentration of 2×10 8 Each / mL, the injection dose is 1mL, the immunization method is multi-point injection, immunization 3 times, after the anti-tumor and cell antibodies are detected in the rabbit serum, the blood is collected and the serum is separated, and the anti-tumor cells in the serum are separated by a protein purification column Antibody;

[0048]3) Antibody binding to tumor cells: The isolated antibody was added to radiation-inactivated tumor cells at a concentration of 100 μg / mL and incubated for 2 hours to obtain the multivalent antibody-binding tumor cell vaccine CT26 tu...

Embodiment 2

[0049] Example 2 Preparation of Multivalent Antibody Conjugated Lung Cancer Tumor Cell Vaccine

[0050] 1) Inactivation: LL2 (LL2, mouse Lewis lung cancer cells) tumor cells in the logarithmic growth phase are treated with X-ray irradiation (100Gy), so that they cannot proliferate but maintain a living state.

[0051] 2) Preparation of tumor cell antibody: immunize rabbits with radiation-inactivated LL2 tumor cells at a concentration of 5×10 8 Each / mL, the injection dose is 1mL, the immunization method is multi-point injection, immunization 3 times, after the anti-tumor and cell antibodies are detected in the rabbit serum, the blood is collected and the serum is separated, and the anti-tumor cells in the serum are separated by a protein purification column Antibody;

[0052] 3) Combination of antibody and tumor cells: the isolated antibody was added to radiation-inactivated tumor cells at a concentration of 1000 μg / mL and incubated for 2 hours to obtain the multivalent antibo...

Embodiment 3

[0053] Example 3 Preventive immune effect of multivalent antibody combined with colon cancer tumor cell vaccine

[0054] 1) Tumor inhibition rate

[0055] See the specific process Figure 8 a, the multivalent antibody conjugated tumor cell vaccine CT26 tumor vaccine prepared in Example 1 was subcutaneously injected into mice at the 1st, 2nd, 3rd, and 4th weeks, and the immunization dose was 10 7 One per 100 μL, one week after immunization, inject CT26 tumor to establish subcutaneous tumor model, and the inoculation volume is 10 7 indivual.

[0056] One week later, tumor volume and mouse body weight were measured every other day to generate a curve.

[0057] Table 1 Mouse tumor volume data

[0058] Volume Day 0 2 4 6 8 10 12 unimmunized group V(mm 3 )

60.67 225.44 425.95 615.22 750.88 1375.94 1834.61 immune group V(mm 3 )

58.69 185.66 215.09 375.68 458.37 687.00 1350.68

[0059] Depend on figure 1 As can be seen f...

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Abstract

The invention belongs to the technical field of immune treatment, and particularly relates to a tumor cell vaccine and a preparation method thereof. The technical problem to be solved by the inventionis that the immunogenicity of the tumor cell vaccine is not strong. According to the technical scheme, the preparation method of the tumor cell vaccine comprises the following steps of (1) performinginactivating; (2) obtaining an immune antibody; and (3) performing incubating. The tumor inactivated vaccine is prepared in a mode that antibody is combined with tumor cells, the vaccine can fully mobilize cellular immunity and humoral immunity, and tumor growth can be effectively prevented and inhibited.

Description

technical field [0001] The invention belongs to the technical field of immunotherapy, and in particular relates to a tumor cell vaccine and a preparation method thereof. Background technique [0002] Immunotherapy is a branch of biological therapy, which has been developed into an important tumor treatment method so far. [0003] Tumor vaccines are currently a research hotspot in the field of tumors at home and abroad. As an active immunotherapy method for tumors, they play an increasingly important role in the treatment of cancer patients. In recent years research has made gratifying progress, such as prostate cancer vaccine. Different from traditional vaccines, tumor vaccines use tumor cells as the main body of the vaccine, supplemented with immune adjuvants or genetically engineered vaccines, and have complete and comprehensive antigens. They not only focus on tumor prevention, but more importantly, they are used for tumor treatment. Tumor cell vaccines contain all the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K39/395A61P35/00C07K16/06C07K16/30
CPCA61K39/0011A61K39/39558A61K2039/57A61K2039/575A61K2039/585A61P35/00C07K16/06C07K16/30
Inventor 王震玲陈飞
Owner SICHUAN UNIV
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