Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-throughput rapid detection method for perfluoro and polyfluoro compounds in serum

A technology of polyfluorinated compounds and detection methods, which is applied in the field of high-throughput rapid detection of perfluorinated and polyfluorinated compounds in serum, can solve the problems of high requirements for detection equipment and increased detection equipment procurement, use and maintenance costs, etc. Achieve stable and reliable analysis results, improve quantitative results, and achieve simple instrument routines

Inactive Publication Date: 2020-09-04
JIANGXI ACADEMY OF SCI
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology provides a method for rapid detection of perfluorinated and polyfluorinated compounds in human serum, but this method has high requirements for detection equipment. Although it saves labor costs, it significantly increases the cost of purchase, use and maintenance of detection equipment.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-throughput rapid detection method for perfluoro and polyfluoro compounds in serum
  • High-throughput rapid detection method for perfluoro and polyfluoro compounds in serum
  • High-throughput rapid detection method for perfluoro and polyfluoro compounds in serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Instruments and reagents used in this embodiment

[0028] 1. Analytical instruments: high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS, Agilent Company), Allegra X-30R centrifuge (Beckman Company), microplate shaker (Thermo Fisher Company), microwell Plate Nitrogen Blowing Apparatus (Organomation Company), Vacuum Freeze Drying Apparatus (Ningbo Xinzhi Biotechnology Company), Constant Temperature Digital Display Water Bath (Xicheng Xinrui Instrument Factory, Jintan District).

[0029] 2. Experimental reagents: MPFACs-Mix internal standard indicators (including 13 C 4 -PFBA,13 C 2 -PFHxA, 18 o 2 -PFHxS, 13 C 4 -PFOS, 13 C 4 -PFOA, 13 C 5 -PFNA, 13 C 2 -PFDA, 13 C 2 -PFUdA, 13 C 2 -PFDoA) was purchased from Wellington Company; sodium dodecyl sulfate (SDS) and sodium carbonate buffer solution (Na 2 CO 3 and NaHCO 3 Aqueous solution, the concentration is 0.25mol / L, pH=10) are excellent grade; tetrabutyl ammonium hydrogen sulfate (...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of biological detection, in particular to a high-throughput rapid detection method for perfluoro and polyfluoro compounds in serum. The method comprises the following steps: (1) serum pretreatment: adding lauryl sodium sulfate into sampled serum for pretreatment; (2) ion pair extraction: taking the pretreated serum to a 96-well plate for methyl tert-butyl ether centrifugal extraction; (3) ultrasonic extraction: freeze-drying the residual solution after extraction in the step (2), and then adding acetonitrile for ultrasonic extraction; (4) high-flux purification: adding the methyl tert-butyl ether extracting solution obtained in the step (2) into a PRiME HLB 96 pore plate, leaching by using the acetonitrile extracting solution obtained in the step (3), blowing methanol to a constant volume by using nitrogen, and centrifuging; and (5) transferring the supernatant obtained after centrifugation in the step (4) to a new 96-well plate for chromatography-mass spectrometry detection. The method has the advantages of high flux, low cost, rapidness and the like, and can be efficiently used for detecting the PFASs in environmental monitoring.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a high-throughput rapid detection method for perfluorinated and polyfluorinated compounds in serum. Background technique [0002] Perfluorinated and polyfluorinated compounds (PFASs) are linear or branched organic compounds in which the hydrogen atoms on the carbon chain are partially or completely replaced by fluorine atoms. Due to their excellent chemical stability and surface activity, they are often used as additives widely. Used in fire extinguishing agents, lubricants, coatings, toiletries, photosensitive material surface treatment agents, textile and leather finishing agents and other fields. Globally, PFASs pollution is very common, and studies have shown that they are widely detected in environmental media (atmosphere, water and soil), plants, animals and human samples. Toxicology studies have found that PFASs are bioaccumulative and potentially biotoxic, which may c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/062
Inventor 刘煜王启宇涂文清吴永明杨春燕
Owner JIANGXI ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com