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Micro-fluidic chip for detecting survivable organisms in water body and detection method

A microfluidic chip and biological detection technology, applied in fluid controllers, chemical instruments and methods, laboratory containers, etc., can solve the problems of unsuitable detection of coliform bacteria, failure to achieve effectiveness, long detection cycle, etc. problems, to achieve efficient and reliable detection, reduce the risk of sample contamination, and low cost

Pending Publication Date: 2020-09-04
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection of microorganisms, especially bacteria, mainly uses methods such as culture, chemiluminescence, bioluminescence, mass spectrometry and polymerase chain reaction (PCR). These methods have the disadvantages of long detection period, complicated procedures, and high price.
The 3M microbiological test piece has been applied in the sanitation monitoring of ballast water, and it was found that it is suitable for the determination of the total number of bacteria in water, but it is not suitable for the detection of coliform bacteria in ballast water
Due to the uncertainty of the growth rate of microorganisms and the time of water discharge, the effectiveness of microbial detection has not been achieved.

Method used

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  • Micro-fluidic chip for detecting survivable organisms in water body and detection method
  • Micro-fluidic chip for detecting survivable organisms in water body and detection method
  • Micro-fluidic chip for detecting survivable organisms in water body and detection method

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Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1: the preparation of chip

[0039] Such as figure 1 As shown, the microfluidic chip used for the detection of survivable organisms in the water body in this embodiment is fabricated by using the "single exposure" technique and the ultraviolet lithography method. After the silicon wafer is treated with a sulfuric acid hydrogen peroxide solution, washed and dried, a layer of SU-82035 negative photoresist is cast on the surface with a glue spreader at a speed of 3000rpm, and baked at 65°C for 10min and at 95°C for 4min Afterwards, after ultraviolet exposure, secondary curing, development, and nitrogen blow-drying, the patterns of each chamber and microchannel network are obtained. Then silanize to make the surface of the mold hydrophobic, polydimethylsiloxane (PDMS) prepolymer and initiator are mixed at a mass ratio of 10:1 to cover the mold, and after removing air bubbles, place it at 75°C for curing. The polymerized PDMS sheet is carefully peeled off, cut i...

Embodiment 2

[0040] Embodiment 2: the structure of the chip

[0041] Such as figure 1 As shown, the microfluidic chip used for the detection of viable organisms in water in this embodiment includes a channel layer 4, a dielectric layer 2 and a dielectric layer bonded sequentially from top to bottom. The base layer 1; the channel layer 4 is provided with an inlet unit, a bacterial electrophoresis control unit and an outlet unit connected in sequence; the inlet unit and the outlet unit all sequentially pass through the channel layer 4 and the dielectric layer 2 .

[0042] The inlet unit includes a bacteria mixture tank 5, a buffer tank 7 and a side channel 6; the outlet unit includes two waste tanks; the bacteria electrophoresis control unit includes a main channel 3, 8 groups of metal microelectrodes and a flat plate drive electrode; the bacterial mixture tank 5 is arranged at one end of the main channel 3, and the two waste liquid tanks are arranged at the other end of the main channel 3...

Embodiment 3

[0053] Embodiment 3: dielectrophoresis technology is used for bacterial detection and separation

[0054] An electrode system is installed at the bottom of the device, which produces different driving effects on different kinds of bacteria during detection. Such as Figure 4 The bacteria are driven by the electrode on the bottom side of the sample tank, so that there is an axial mutual separation between different bacteria, that is, different bacteria are separated due to differences in physical and chemical properties, and they are concentrated in a certain area to move forward. After separation, the metal microelectrodes 8 staggered on both sides of the main channel 3 perform electrical focusing on the separated bacterial zones, so that various bacteria are concentrated in the center of each zone, which is convenient for detection. The sensing electrodes on both sides of the detection area monitor in real time, and the change of conductivity of the solution in the conductio...

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Abstract

The invention discloses a micro-fluidic chip for detecting survivable organisms in a water body, and relates to the technical field of environmental monitoring. The micro-fluidic chip comprises a channel layer, a dielectric layer and a substrate layer which are sequentially bonded from top to bottom. An inlet unit, a bacterial electrophoresis control unit and an outlet unit which are sequentiallycommunicated are arranged in the channel layer; and the inlet unit and the outlet unit penetrate through the channel layer and the dielectric layer in sequence. The invention further provides a detection method of the micro-fluidic chip for detecting the survivable organisms in the water body. The micro-fluidic chip has the advantages of single bacterium analysis capability, high integration and simple operation. According to the present invention, the microfluidic chip devices has advantages of simple production, easy operation and fluid flowing state control in the channel, can achieve qualitative and quantitative detection analysis on the variety and the number of bacteria in water, especially ballast water, and is suitable for survivable biological analysis and drug monitoring of environmental water, especially ballast water.

Description

technical field [0001] The invention relates to the technical field of environmental monitoring, in particular to a microfluidic chip and a detection method for detecting survivable organisms in water bodies. Background technique [0002] Bacterial pollution indicators are a series of indicators used to measure whether water sources are polluted by bacteria and describe the degree of pollution. Humans often come into contact with pathogenic bacteria in water through drinking, touching, etc., causing harm to human health. The most common diseases include cholera, typhoid, and dysentery. In order to ensure the hygienic quality of water bodies, different types of water quality should be tested for microorganisms. Due to the low content of pathogenic bacteria in the water, the detection steps are complicated and time-consuming, so it is difficult. Therefore, all countries in the world generally detect unified sanitary indicator bacteria first, and then detect various pathogeni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N27/447
CPCB01L3/5027B01L3/50273G01N27/447B01L2200/0647B01L2300/0819B01L2400/0421B01L2400/0415
Inventor 林雪霞王晨境
Owner HUAQIAO UNIVERSITY
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