3D bioprinted active biofilm for improved amic technology cartilage repair and its preparation method

Abstract

A modified AMIC bio-3D printed active biofilm for cartilage repair, characterized in that: the biofilm uses sodium alginate / gelatin / hyaluronic acid as raw materials to prepare a mixed hydrogel, and the hydrogel is mixed with pre-cartilage Somatic cells and fibronectin, using depositional bio-3D printing technology to construct a porous hydrogel biofilm, and chemically cross-linked to enhance mechanical properties by soaking in calcium chloride. The materials used in the invention are all natural materials, have low immunogenicity, good biocompatibility, wide sources, and have certain mechanical properties to provide good support for cartilage regeneration. As far as the preparation method is concerned, the gaps of 300-500 microns can promote cartilage regeneration, and the excellent porosity structure can also promote the exchange and communication of substances between cells, which is conducive to the adhesion of cytokines or cells, and is beneficial to cells in its environment. Internal growth and proliferation.

Description

Technical field [0001] The present invention relates to the technical field of biomedical materials, in particular, referring to an active biofilm and preparation method thereof for a biological 3D printing of cartilage repair. Background technique [0002] The transparent cartilage of the joint is lacking blood vessels, nerves, and lymphism. Once damage is difficult to achieve self-healing and easy to develop into degenerative diseases, clinical existing treatment techniques such as bone cartilage transplantation, autologous cartilage cell transplantation, microfractive technology and AMIC technology and other repairs are limited and long-term efficacy is poor. In recent years, tissue engineering technology and biological 3D printing technology have gradually become a new generation of cartilage repair technology. 3D printing technique can accurately control the internal structure of the bracket, construct a chemical similar to the cartilage morphological structure, and control ...

AI Technical Summary

Problems solved by technology

[0006] For large-scale talus osteochondral injuries, the most common method at present is to use allogeneic bone transplantation after the lesion is removed. When the allogeneic bone is taken, the three-dimensional geometry of the amputated recipient's talus lesion is artificially observed and the corresponding part is cut from the allogeneic talus. For repair and reconstruction, this method has strong subjectivity and inaccuracy, and the shape of the talus is irregular, and the size, shape, and radian of each person's talus are not the same. The obtained allogeneic bone cannot be accurately matched and reconstructed with the recipient's talus , the size, shape, and radian of the cut allograft bone and the recipient talus are likely to be inconsistent, and the articular surface is uneven, resulting in abnormal stress in the joint after surgery, osteoarthritis, etc., resulting in poor curative effect or surgical failure. Therefore, a Cartilage 3D printer capable of precise repairs

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