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Efficient environmentally-friendly production and extraction process of amino acid

A technology of green production and extraction process, applied in the field of bioengineering, can solve problems such as increasing operating procedures, increasing costs, reducing production efficiency, etc., to achieve the effect of improving production efficiency, maintaining continuous growth, and maintaining cell activity

Inactive Publication Date: 2020-08-25
赵兰坤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, each fermentation cycle requires microbial seed culture solution, and the seed culture of microorganisms increases the cost of a fermentation cycle, increases operating procedures, and reduces production efficiency

Method used

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  • Efficient environmentally-friendly production and extraction process of amino acid
  • Efficient environmentally-friendly production and extraction process of amino acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Alanine-containing fermentation obtained by Escherichia coli fermentation

[0036] 1. Experimental method

[0037] Bacterial species: Escherichia coli E.coli MG1655 was used as the test strain.

[0038] Activation of bacteria:

[0039] Take the strain out of the bacteria preservation tube from the -80°C refrigerator, activate and culture it on the slant of the test tube for 2 generations, and cultivate it in a 32°C incubator for 16 hours until the slant of the test tube is covered with a lawn;

[0040] Seed culture:

[0041] The activated strains were subcultured and inoculated into eggplant-shaped bottles, cultured at 32°C for 12 hours, then washed with sterile water and inoculated into 5L seed tanks containing 3L seed medium for primary seed expansion, and the temperature was automatically controlled at 32±0.5 ℃, adjust dissolved oxygen to 30±10% by ventilation flow rate and stirring speed, automatically add 25% ammonia water by coupling pH electrode to maintain pH...

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PUM

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Abstract

The invention belongs to the technical field of bioengineering, and relates to an amino acid efficient environmentally-friendly production extraction process. The process comprises the following stepsof: collecting an L-alanine fermentation broth, performing centrifuging, and collecting a supernatant; carrying out microfiltration on the supernatant through a ceramic membrane, and collecting filtrate; adding activated carbon into the filtrate for decolorization, performing filtering by a plate frame, and collecting a decolorized solution; exchanging L-alanine onto resin through strongly acidiccation exchange resin, then completely eluting the L-alanine on the resin with ammonia water, and carrying out concentration crystallization, centrifugation and drying to obtain a finished product. According to the process, fermentation production intensity can be improved, so that the production cost of fermented alanine is reduced.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to an efficient green production and extraction process of amino acids. Background technique [0002] L-alanine is colorless to white crystalline powder, soluble in water and ethanol, insoluble in ether and acetone. It is mainly used in biochemical research, tissue culture, liver function test, flavor enhancer, can increase the seasoning effect of condiments, and can also be used as a sour taste corrector to improve the sour taste of organic acids. The use of alanine in the field of medicine has also been discovered. Clinical studies have shown that alanine can prevent kidney stones, assist glucose metabolism, help alleviate hypoglycemia, and improve body energy. Alanine is also widely used as a raw material for the synthesis of new sweeteners and some chiral drug intermediates. [0003] At present, alanine is usually produced industrially by chemical method, bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C227/40C07C229/08C12P13/06C12N1/38C12N1/20C12R1/19
CPCC07C227/40C12P13/06C12N1/38C12N1/20C07C229/08
Inventor 赵兰坤赵凤良王小平魏冉冉刘世周程士清
Owner 赵兰坤
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