Efficient environmentally-friendly production and extraction process of amino acid
A technology of green production and extraction process, applied in the field of bioengineering, can solve problems such as increasing operating procedures, increasing costs, reducing production efficiency, etc., to achieve the effect of improving production efficiency, maintaining continuous growth, and maintaining cell activity
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[0035] Alanine-containing fermentation obtained by Escherichia coli fermentation
[0036] 1. Experimental method
[0037] Bacterial species: Escherichia coli E.coli MG1655 was used as the test strain.
[0038] Activation of bacteria:
[0039] Take the strain out of the bacteria preservation tube from the -80°C refrigerator, activate and culture it on the slant of the test tube for 2 generations, and cultivate it in a 32°C incubator for 16 hours until the slant of the test tube is covered with a lawn;
[0040] Seed culture:
[0041] The activated strains were subcultured and inoculated into eggplant-shaped bottles, cultured at 32°C for 12 hours, then washed with sterile water and inoculated into 5L seed tanks containing 3L seed medium for primary seed expansion, and the temperature was automatically controlled at 32±0.5 ℃, adjust dissolved oxygen to 30±10% by ventilation flow rate and stirring speed, automatically add 25% ammonia water by coupling pH electrode to maintain pH...
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