Method for loading medicines on tumor targeted amorphous calcium phosphate fluorescence nanocomposite materials

An amorphous calcium phosphate, fluorescent nanomaterial technology, applied in nanomedicine, antitumor medicine, nanotechnology and other directions, can solve the problems of tissue and organ damage, unsatisfactory curative effect, damage and other problems, achieve good biocompatibility, The effect of realizing the integration of diagnosis and treatment and good biological imaging effect

Active Publication Date: 2020-08-11
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the blood concentration is too high, it will cause damage to the body, and if it is lower than a certain concentration, the desired effect cannot be achieved.
Secondly, traditional drug delivery methods may also cause damage to normal tissues and organs while achieving therapeutic effects

Method used

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  • Method for loading medicines on tumor targeted amorphous calcium phosphate fluorescence nanocomposite materials
  • Method for loading medicines on tumor targeted amorphous calcium phosphate fluorescence nanocomposite materials
  • Method for loading medicines on tumor targeted amorphous calcium phosphate fluorescence nanocomposite materials

Examples

Experimental program
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Effect test

Embodiment 1

[0035] This example provides a method for tumor-targeting amorphous calcium phosphate fluorescent nanocomposite HA-FCNs / ACP, which includes the following steps: firstly synthesize partially carbonized hyaluronic acid (HA-FCNs) by high temperature dehydration, and prepare the HA -FCNs were added to the preparation process of ACP, and the amorphous calcium phosphate fluorescent composite material (HA-FCNs / ACP) was synthesized by co-precipitation method. Specific steps are as follows:

[0036] (1) Dissolve 0.3 g of hyaluronic acid in 10 mL of deionized water, add 10 mL of concentrated sulfuric acid into the aqueous solution of hyaluronic acid under the condition of stirring in an oil bath at 230 °C, stir for 1.5 min, and dialyze with a molecular weight of 500 Da The bag was dialyzed for three days and freeze-dried to obtain HA-FCNs fluorescent material.

[0037] The prepared HA-FCNs nanoparticles were tested by fluorescence emission spectrum and ultraviolet spectrum ( figure 1 ...

Embodiment 2

[0042] (1) Dissolve 0.4 g of hyaluronic acid in 10 mL of deionized water, add 15 mL of concentrated sulfuric acid into the aqueous solution of hyaluronic acid under the condition of stirring in an oil bath at 230 °C, stir for 2 min, and dialyze with a molecular weight of 2000 Da The bag was dialyzed for three days and freeze-dried to obtain HA-FCNs fluorescent material.

[0043] (2) Prepare 0.034 mol / L 29 mL calcium nitrate aqueous solution, 0.024 mol / L 29 mL diammonium hydrogen phosphate aqueous solution, 1 mol / L 50 mL NaOH solution, 700 μg / mL 30 mL HA-FCNs aqueous solution and 0.6 mg / L mL 10 mL DOX aqueous solution, in which, ultrasonically disperse DOX aqueous solution (ultrasonic conditions: probe ultrasonic power 500 W, ultrasonic 2 s, intermittent 3 s). The HA-FCNs aqueous solution was added dropwise to the calcium nitrate solution at 35 ºC, and after blending and stirring for 1.5 h, the DOX aqueous solution was added dropwise to the above mixture, and stirred for 1 h. ...

Embodiment 3

[0046] (1) Dissolve 0.2 g of hyaluronic acid in 10 mL of deionized water, add 15 mL of concentrated sulfuric acid into the aqueous solution of hyaluronic acid under the condition of stirring in an oil bath at 240 °C, stir for 1 min, and dialyze with a molecular weight of 3500 Da The bag was dialyzed for three days and freeze-dried to obtain HA-FCNs fluorescent material.

[0047] (2) Preparation of Cur-loaded composite materials: 0.034 mol / L 29 mL calcium nitrate aqueous solution, 0.024 mol / L 29 mL diammonium hydrogen phosphate aqueous solution, 1 mol / L 50 mL NaOH solution, 800 μg / mL 30 mL HA-FCNs aqueous solution and 5 mg / mL 10 mL Cur aqueous solution, in which the Cur aqueous solution was dispersed ultrasonically (ultrasonic conditions: probe ultrasonic power 400 W, ultrasonic 3 s, intermittent 3 s). The HA-FCNs aqueous solution was added dropwise to the calcium nitrate solution at 25 ºC, and after blending and stirring for 1 h, the Cur aqueous solution was added dropwise to ...

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Abstract

The invention discloses a method for loading medicines on tumor targeted amorphous calcium phosphate fluorescence nanocomposite materials. The method comprises the steps of firstly with hyaluronic acid (HA) as a raw material, preparing partially-carbonized hyaluronic acid fluorescent materials HA-FCNs (HA fluorescence carbon nanoparticles) by a high temperature dehydration method; and then with calcium nitrate as a calcium source, diammonium hydrogen phosphate as a phosphorus source and HA-FCNs as a marker, preparing the tumor targeted amorphous calcium phosphate fluorescence nanocomposite materials (HA-FCNs/ACP) by a coprecipitation method. Besides, curcumin (Cur) is used as a lyophobic medicine model, doxorubicin hydrochloride (DOX) is used as a hydrophilic medicine model, and loading oftwo medicines is realized. The medicine carrier prepared by the method disclosed by the invention is good in biocompatibility, has obvious biological imaging and pH responsiveness, has targeting properties on cancer cells (A549 cells), has obvious enzymatic release reactions in cancer cell internal environment, and significantly reduces cancer cell viability.

Description

technical field [0001] The invention relates to a method for loading drugs on a tumor-targeted amorphous calcium phosphate fluorescent nanocomposite material, and belongs to the technical field of biological materials. Background technique [0002] With the traditional dosage form and administration method, the drug enters the body and releases rapidly, and then drops rapidly after reaching the peak value, resulting in an obvious peak-valley phenomenon in the blood drug concentration. When the blood drug concentration is too high, it will cause damage to the body, and when it is lower than a certain concentration, the ideal curative effect cannot be achieved. Secondly, traditional drug delivery methods may also cause damage to normal tissues and organs while achieving therapeutic effects. Therefore, the development of a new type of drug carrier needs to extend the drug action time, improve the selectivity of the drug to the lesion site, improve the efficacy of the drug, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K47/69A61K47/02A61K31/704A61K31/12A61P35/00B82Y5/00B82Y20/00B82Y30/00B82Y40/00
CPCA61K49/0054A61K47/6939A61K49/0093A61K31/12A61K31/704A61P35/00B82Y5/00B82Y20/00B82Y30/00B82Y40/00A61K47/02
Inventor 牛宝龙李文凤贾剑红连小洁王慧芳高向华
Owner TAIYUAN UNIV OF TECH
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