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Newcastle disease live vaccine adjuvant and preparation method thereof

A technology for chicken Newcastle disease and live vaccines, applied in biochemical equipment and methods, vaccines, veterinary vaccines, etc., can solve the problems that vaccines and drugs cannot achieve good results, the therapeutic effect cannot meet the expected requirements, and the efficacy of drugs is slow. , to achieve the effect of improving humoral immune response, improving cellular immune response, and reducing side effects after vaccine immunization

Pending Publication Date: 2020-08-07
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing adjuvant of chicken Newcastle disease vaccine has the following problems in the process of treating chickens: the adjuvant drug composition for the treatment of chicken Newcastle disease is relatively single, the drug effect is slow and the therapeutic effect cannot meet the expected requirements, which is easy to cause a series of diseases. The symptoms of the disease, resulting in vaccine drugs can not achieve better results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A live Newcastle disease vaccine adjuvant comprising the following ingredients in weight percent raw materials: buffer 70%, squalane 8%, squalene 8%, Pluronic 4%, emulsifier 2%, hydroxymethyl cellulose 3%, dimethyl dioctadecyl ammonium bromide 3%, polycaprolactone 2%.

[0021] Further improved, the emulsifier is selected from one or more of castor oil polyoxyethylene ether EL60, F-188, ELP, TWEEN-80, TWEEN-60, propylene glycol fatty acid ester, lanolin, glycerin fatty acid ester The combination.

[0022] Further improved, the buffer is one of deionized water, distilled water, physiological saline, phosphate buffer or sodium citrate buffer.

[0023] Further improved, the preparation method includes the following steps:

[0024] S1: Pour squalane, squalene, Pluronic, hydroxymethyl cellulose, dimethyl dioctadecyl ammonium bromide and part of the buffer solution into the container according to the above ratio for pre-mixing treatment, using agitator Stir and mix the abov...

Embodiment 2

[0032] A live chicken Newcastle disease vaccine adjuvant, comprising the weight percentage raw materials of the following components: buffer 75%, squalane 10%, squalene 10%, Pluronic 5%, emulsifier 3%, hydroxymethyl cellulose 4%, Dimethyl Dioctadecyl Ammonium Bromide 4%, Polycaprolactone 3%.

[0033] Further improved, the emulsifier is selected from one or more of castor oil polyoxyethylene ether EL60, F-188, ELP, TWEEN-80, TWEEN-60, propylene glycol fatty acid ester, lanolin, glycerin fatty acid ester The combination.

[0034] Further improved, the buffer is one of deionized water, distilled water, physiological saline, phosphate buffer or sodium citrate buffer.

[0035] Further improved, the preparation method includes the following steps:

[0036] S1: Pour squalane, squalene, Pluronic, hydroxymethyl cellulose, dimethyl dioctadecyl ammonium bromide and part of the buffer solution into the container according to the above ratio for pre-mixing treatment, using agitator Stir...

Embodiment 3

[0044] A live chicken Newcastle disease vaccine adjuvant, comprising the weight percentage raw materials of the following components: buffer 60%, squalane 5%, squalene 5%, Pluronic 3%, emulsifier 1%, hydroxymethyl cellulose 2%, Dimethyl Dioctadecyl Ammonium Bromide 2%, Polycaprolactone 1%.

[0045]Further improved, the emulsifier is selected from one or more of castor oil polyoxyethylene ether EL60, F-188, ELP, TWEEN-80, TWEEN-60, propylene glycol fatty acid ester, lanolin, glycerin fatty acid ester The combination.

[0046] Further improved, the buffer is one of deionized water, distilled water, physiological saline, phosphate buffer or sodium citrate buffer.

[0047] Further improved, the preparation method includes the following steps:

[0048] S1: Pour squalane, squalene, Pluronic, hydroxymethyl cellulose, dimethyl dioctadecyl ammonium bromide and part of the buffer solution into the container according to the above ratio for pre-mixing treatment, using agitator Stir an...

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Abstract

The invention provides a newcastle disease live vaccine adjuvant. The newcastle disease live vaccine adjuvant comprises the following raw materials in percentage by weight: 60-75% of a buffer solution, 5%-10% of squalane, 5%-10% of squalene, 3%-5% of pluronic, 1%-3% of an emulsifier, 2%-4% of hydroxymethyl cellulose, 2%-4% of dimethyl dioctadecyl ammonium bromide and 1%-3% of polycaprolactone. Theadjuvant composition can remarkably improve humoral immune response and cellular immune response of the newcastle disease vaccine, not only can generate a relatively high antibody level in a short time, but also can stimulate an organism to generate efficient protective force for a long time, when the adjuvant composition is used for preparing vaccines, large emulsification equipment is not needed, the vaccine preparation process is stable, and the side reaction after vaccine immunization is small.

Description

technical field [0001] The invention relates to the technical field of animal vaccine adjuvants, in particular to a live Newcastle disease vaccine adjuvant and a preparation method thereof. Background technique [0002] Chicken Newcastle disease is an acute septicemia infectious disease caused by a virus. Commonly known as "chicken plague", the so-called Asian chicken plague. The disease can occur throughout the year, especially in cold and climate-changing seasons. Chickens of various ages can be infected, and chickens aged 20 to 60 days are the most susceptible and have a high mortality rate. The main features are dyspnea, neurological disturbance, mucosal and serosal hemorrhage and necrosis. [0003] Vaccine adjuvants are substances that can non-specifically change or enhance the body's specific immune response to antigens and play an auxiliary role. The adjuvant can induce the body to produce a long-term and efficient specific immune response, improve the body's prot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/17A61P31/14
CPCA61K39/12A61K39/39A61K2039/5254A61K2039/552A61K2039/55511A61K2039/55566A61K2039/55583A61P31/14C12N2760/18134
Inventor 朱亚露
Owner JIANGSU ACAD OF AGRI SCI
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