Method for inducing polyploid plants of hemsleya chinensis by using adventitious buds and application of method

Abstract

The present invention relates to a polyploid culture method of traditional Chinese medicinal materials and particularly to a method for inducing polyploid plants of hemsleya chinensis by using adventitious buds and an application of the method. The method comprises the specific processes: 1) aseptic culture of adventitious buds; 2) polyploid induction; 3) chimera isolation culture; 4) polyploid screening; 5) polyploid expansion and 6) polyploid rooting. The method of aseptic tissue culture is used to conduct polyploid breeding, can provide a large number of stable hemsleya chinensis polyploidseedlings in a short period of time in planting hemsleya chinensis polyploids. Colchicine is used to conduct polyploid induction of sterile adventitious buds of the hemsleya chinensis, then screeningand purification cultivation are conduct to obtain hemsleya chinensis germplasm through a morphological identification method and a chromosome tableting method and a polyploid induction rate is 26%. The method induces the polyploids produced by germplasm with female parent hemsleyadin content of 1.46%, after two years of planting comparison (generally a planting cycle is 3-4 years), compared withplanted diploid tissue culture seedlings with the same female parents in the same period, the hemsleyadin content is 0.2% higher, and the method effectively improve application value of medicinal materials and market price of the medicinal materials.

Description

technical field [0001] The invention relates to a method for cultivating polyploids of Chinese medicinal materials, belonging to the fields of plant crop breeding and planting of Chinese medicinal materials, in particular to a method for cultivating polyploids of snow gall and its application. Background technique [0002] Snow gall is a plant of the genus Snow gall of Cucurbitaceae, a perennial climbing herb with enlarged tubers, mainly distributed in subtropical to temperate regions of Asia, and born in sparse forests or shrubs at an altitude of 800-3500 meters. There are 31 species of the genus Snow Gall in China, and 26 species in Yunnan. The 1977 edition of "Chinese Pharmacopoeia" included Hemsleya amabils Diels (Hemsleya amabils Diels) and the dried roots of several plants of the same genus as medicinal materials of snow gall, and "Chinese Materia Medica" included Hemsleya amabils (H.chinensis Cogn.ex Forb.et Hemsl) Qulian (H.amabils Diels) and large-seed snow gall (H...

AI Technical Summary

Problems solved by technology

Modern medical research has shown that snow gallbladder contains various active ingredients such as snow gallin (the general name of snow gallbladder and snow gallbladder B), snow gall saponin, oleanolic acid, etc., among which snow gallbladder is the most valuable and The representative active ingredient has antibacterial effect, anti-tumor effect, in vitro anti-HIV activity, etc., and has low toxicity. It has the reputation of "natural antibiotic". The content of snow gallin in gallbladder medicines varies greatly (1 part per million to 1.5 percent), which greatly affects the efficacy of snow gallbladder