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Fast-growing and high-quality grass carp snps and its application

An excellent technology for grass carp, applied in the field of DNA markers, can solve problems affecting gene translation efficiency, protein expression level differences, and gene transcription efficiency, etc., and achieve the effect of simple operation, excellent body width, and easy to be widely used

Active Publication Date: 2022-01-04
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that SNPs in the promoter region of functional genes may affect gene transcription efficiency, which in turn affects gene translation efficiency, resulting in differences in protein expression

Method used

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  • Fast-growing and high-quality grass carp snps and its application
  • Fast-growing and high-quality grass carp snps and its application
  • Fast-growing and high-quality grass carp snps and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 SNP marker

[0054] The grass carp gene sequence is as follows, one SNP site is found at the 175th base of SEQ ID NO.1, the 4139th base of SEQ ID NO.1, and the 4516th base of SEQ ID NO.1.

[0055] SNP1 has allele C or G at base 175 of SEQ ID NO.1, forming three genotypes CC, CG and GG; SNP2 has allele at base 4139 of SEQ ID NO.1 Gene G or C constitutes three genotypes CC, CG and GG; SNP3 has an allele G or A at base 4516 of SEQ ID NO.1, constituting two genotypes AG and GG.

Embodiment 2

[0056] Example 2 SNP site screening, verification associated with growth traits

[0057] 1. Sample Genomic DNA Extraction

[0058] Grass carp fin ray tissue was taken, and its genomic DNA was extracted (Guangzhou Meiji Biological Marine Animal Tissue Genomic DNA Extraction Kit), and the DNA integrity, DNA quality and concentration were detected.

[0059] 2. Primer Design and Synthesis

[0060] A gene fragment was obtained from the grass carp genome database. In order to verify the accuracy of the sequence and screen SNPs, 5 pairs of PCR primers P1-P5 (see Table 1 for the sequence) were designed using Primer Premier 5.0 software to amplify 24 populations from different geographical origins. , PCR primers commissioned by Guangzhou Aiji Biotechnology Co., Ltd. to synthesize.

[0061] Table 1 Sequencing primers for screening gene fragment SNPs sites

[0062]

[0063] 3. PCR amplification reaction system:

[0064]

[0065] PCR amplification reaction conditions: the amplific...

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Abstract

The invention discloses fast-growing and high-quality grass carp SNPs and their application. The invention detects three SNP markers of grass carp. SNP1 is located at the 175th base of the grass carp gene SEQ ID NO.1, and SNP2 is located at the 4139th base of the grass carp gene SEQ ID NO.1. base, SNP3 is located at the 4516th base of the grass carp gene SEQ ID NO.1. Through the screening of SNP sites, under the same breeding conditions, fast-growing and slender grass carp individuals with excellent weight, body length, body height and body width and low fatness can be quickly obtained. The invention ensures the reliability of the detection results, does not need cumbersome operations for sequence analysis, and improves the processing efficiency and accuracy. The invention can be effectively used for molecular marker assisted breeding of grass carp, and accelerates the breeding process of grass carp.

Description

technical field [0001] The invention belongs to the technical field of DNA markers, and particularly relates to fast-growing and high-quality grass carp SNPs and applications thereof. Background technique [0002] Grass carp (Ctenopharyngodon idella) belongs to the subfamily Leuciscinae and the genus Ctenopharyn godon. It has the advantages of fast growth, delicious meat, and high nutritional value. Grass carp farming has developed rapidly and healthily in recent years, not only providing a large amount of high-quality animal protein for people in my country and even developing countries, but also driving the development and growth of related industries such as feed processing, transportation and sales. So far, grass carp are mostly directly domesticated from wild species, lack of targeted breeding, resulting in problems such as poor quality of parents, slow growth of offspring, uneven specifications, poor disease resistance, etc., causing significant economic losses to soci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/12C12Q1/6888C12Q1/686C12N15/11
CPCC07K14/461C12Q1/6888C12Q1/686C12Q2600/156C12Q2600/124C12Q2537/143
Inventor 樊佳佳马冬梅朱华平白俊杰姜鹏李胜杰
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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