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Method for constructing biological 3D printing skin microcell model containing skin appendage

A 3D printing, micro-unit technology, applied in medical science, prosthesis, additive processing, etc., can solve problems such as construction

Active Publication Date: 2020-06-26
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some 3D printed skin materials have been prepared, it is still not possible to construct complete blood vessels and hair follicles and other appendages in the skin materials.

Method used

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  • Method for constructing biological 3D printing skin microcell model containing skin appendage
  • Method for constructing biological 3D printing skin microcell model containing skin appendage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for constructing a biological 3D printing skin microunit model containing skin appendages, comprising the steps of:

[0024] 1) Prepare a bio-3D printing glue block containing seed cells of sweat gland tissue, and plant hair follicles and sebaceous gland hanging drops on the bio-3D printing glue block;

[0025] 2) Inducing culture using an induction medium on the biological 3D printing block; wherein, the induction medium includes a sweat gland induction medium, a hair follicle induction medium and a sebaceous gland induction medium;

[0026] 3) After 5-10 days of induced culture, add epidermal stem cells on the surface of the bio-3D printing glue block, and perform air-liquid plane culture to form bio-3D printed skin microunits.

[0027] Wherein, the seed cells in step 1) include one or more of skin precursor cells, skin appendage precursor cells, adult stem cells or induced pluripotent stem cells. The skin precursor cells, skin appendage precursor cells, adu...

Embodiment 2

[0031] The preparation of biological 3D printing glue blocks comprises the following steps:

[0032] 1.1) Collect seed cells containing sweat gland tissue, mix with printing materials evenly, and prepare bio-3D printing ink;

[0033] Among them, the printing materials include: lactic acid-glycolic acid copolymer, polylactic acid, polycaprolactone, racemic polylactic acid-polydisperse methylene carbonate copolymer, nano-beta-tricalcium phosphate, nano-hydroxyapatite, nano One or more of calcium phosphate, gelatin, sodium alginate hydrogel or type I collagen hydrogel;

[0034] An example of material preparation for printing:

[0035] Weigh 3.8g of lactic acid-glycolic acid copolymer, dissolve it in 10mg of dichloromethane, stir for 2 hours until the lactic acid-glycolic acid copolymer is completely dissolved, and form a lactic acid-glycolic acid copolymer solution; weigh 3.8g beta-tricalcium phosphate Disperse in the lactic acid-glycolic acid copolymer solution and dissolve co...

Embodiment 3

[0048] The bio-3D printed skin dermis microunits prepared in Example 2 were induced and cultured;

[0049] The 3D bioprinted skin dermis microunits are placed in an incubator at 37°C for 5-10 days, preferably 7 days. The induction medium components include three types, specifically sweat gland induction medium, hair follicle induction medium and sebaceous gland induction medium. In this embodiment, the induction medium uses the same amount of sweat gland induction medium, hair follicle induction medium and sebaceous gland induction medium.

[0050] Specifically, the components of the induction medium:

[0051] Component 1, sweat gland induction medium: DMEN / E12 (1:1) added 10% v / v fetal bovine serum, 10ng / ml epidermal growth factor, 20ng / ml triiodothyronine, 0.64ng Hydrocortisone / ml, ITS 1% v / v;

[0052] Component 2, hair follicle induction medium: DMEN / E12 (1:1) added 5% v / v fetal bovine serum, 20% v / v ITS, 0.2nM / ml triiodothyronine, 20ng Basic fibroblast growth factor pe...

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Abstract

The invention relates to a method for constructing a biological 3D printing skin microcell model containing skin appendages. The method comprises the following steps: 1) preparing a biological 3D printing rubber block containing seed cells of sweat gland tissues, and hanging and planting hair follicles and sebaceous glands on the biological 3D printing rubber block; 2) performing induction cultureon the biological 3D printing rubber block by using an induction culture medium; wherein the induction culture medium comprises a sweat gland induction culture medium, a hair follicle induction culture medium and a sebaceous gland induction culture medium; and 3) after induction culture for 5-10 days, adding epidermal stem cells to a surface of the biological 3D printing rubber block, and performing gas-liquid planar cultivation to form biological 3D printing skin microcell. The skin microcell model prepared by the method realizes synchronous regeneration of dermis and epidermis of the skin and various skin appendages.

Description

technical field [0001] The invention relates to the field of medical model manufacturing and application, in particular to a biological 3D printed skin microunit model containing skin appendages and a preparation method thereof. Background technique [0002] Skin injury is one of the common forms of surgical trauma, but its repair process is often imperfect. For most individuals, skin repair only restores skin integrity, and skin appendages often fail to regenerate. Skin appendages such as sweat glands, hair follicles, and sebaceous glands not only play a very important role in maintaining the homeostasis of the skin, but also have a major impact on the skin function and appearance of the human body. Therefore, how to simultaneously induce the regeneration of skin appendages such as sweat glands and hair follicles during skin wound repair is one of the key issues in the fields of regenerative medicine and trauma surgery. [0003] The regeneration of skin appendages such as...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/18A61L27/50A61L27/60B33Y10/00B33Y70/10B33Y80/00
CPCA61L27/18A61L27/3804A61L27/3813A61L27/3834A61L27/50A61L27/60B33Y10/00B33Y70/00B33Y80/00C08L67/04
Inventor 张熠杰黄沙姚斌李建军付小兵
Owner GENERAL HOSPITAL OF PLA
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