Biofilm for guiding oral bone regeneration and preparation method thereof

A technology of biofilm and oral bone, which is applied in the field of oral absorbable biomaterials, and can solve problems such as poor stability and unsustainable barrier effect

Active Publication Date: 2021-12-07
江苏白衣缘生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] In view of the deficiencies and deficiencies of the existing guided bone regeneration composite membrane, the first purpose of the present invention is to provide a composite film that does not use any chemical cross-linking agent or use physical cross-linking methods, but has good resistance to enzymatic degradation. , a more stable natural GBR collagen film that can act as a long-lasting isolation barrier; this type of natural biofilm must have good biocompatibility, be degradable and absorbable, and have no chemical reagent residue. More importantly, it must be able to overcome The existing natural GBR collagen membrane has poor stability, rapid degradation, and short-lasting barrier effect; it can prevent the gingival soft tissue from entering the bone growth area for a longer period of time, and reserve sufficient space for new bone growth in the bone defect area. Regenerate space, and guide osteoblasts to grow into new bone in the bone defect area, so as to achieve the purpose of bone healing

Method used

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  • Biofilm for guiding oral bone regeneration and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] Example 1: Preparation of layer A, decellularized periosteum (eliminated sow periosteum)

[0118] Specific steps are as follows:

[0119]1. Material collection and pretreatment: select and eliminate sows, whose gestational age is over 8 litters, have been bred for more than 40 months, weigh about 180 kg, and are empty; after slaughter, take the forelegs (humerus, radius, fibula) and remove them physically and mechanically. After the epidermis and fascia layer of the bone, the periosteum layer on the surface of the leg bone is exposed, and the periosteum is cut around the selected periosteum area with a scalpel, and the complete periosteum tissue in the selected area is carefully peeled off with the forceps for periosteum stripping. The isolated periosteum was soaked in 1.0% acetic acid solution for 30 minutes, the ratio of young pig periosteum to acetic acid solution was 1:10, and then soaked in purified water to obtain the A-layer membrane raw material;

[0120] 2. Di...

Embodiment 2

[0125] Example 2: Preparation of layer A, decellularized SIS (cultivated sows)

[0126] The steps are basically the same as in Example 1, the only difference lies in the selection of raw material parts. Example 1 is the periosteum. In this example, the submucosa of the small intestine of sows is eliminated, and the mucosal layer, muscular layer, and serosa of the small intestine and jejunum are removed by mechanical scraping. layer, lymph nodes, etc., to obtain raw material of B layer small intestinal submucosa (SIS). The second difference: during the pretreatment of raw materials, 1.8% acetic acid concentration is used instead of 1.0% acetic acid, and the rest of the unspecified steps and reagents are exactly the same as in Example 1.

Embodiment 3

[0127] Example 3: Preparation of layer A, decellularized dermis (cultivated sows)

[0128] The steps are basically the same as in Example 2, the difference is only in the selection of raw material parts. Example 1 is the periosteum. In this example, the dermis of the sow is eliminated, and the middle skin of the abdomen is taken, fully cleaned, and the fat on the inner surface and the outer layer are mechanically removed. Rinse with deionized water, and take a 0.15mm thick corium with a skin remover.

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Abstract

The present invention relates to a guided bone regeneration (GBR) biofilm, which is characterized in that the layer (layer A) in contact with the oral cavity periodontal soft tissue is a decellularized breeding stock ECM film with good cross-linking To prevent premature degradation of the A layer membrane, so that the isolation effect is more durable; at the same time, the layer (B layer) in contact with the alveolar bone defect is the decellularized ECM membrane of young animals, such as the periosteum of the piglet's leg or the mucosa of the small intestine The lower layer; the B layer derived from young animals contains more bioactive factors (such as HA) and has a strong ability to induce tissue regeneration; this type of GBR membrane has both long-lasting isolation effects and good bone tissue regeneration functions, and is completely degradable and degradable. Absorption; no cross-linking agent is used in production to enhance the anti-enzymolysis performance and avoid cross-linking agent residue; the GBR membrane of the present invention is not only conducive to guiding the high-quality reconstruction of bone tissue, but also inducing its early regeneration, which can take into account both Good and fast.

Description

technical field [0001] The invention belongs to the field of absorbable biomaterials for oral cavity, and in particular relates to a biofilm guiding oral cavity bone regeneration and a preparation method thereof. Background technique [0002] At present, with the continuous improvement of living standards, dental implant restoration has become the first choice for patients with missing teeth. However, the key to the success of dental implant restoration is sufficient alveolar bone stock or sufficient alveolar bone augmentation in the missing tooth. However, Clinically, due to various factors such as age, constitution, inflammation, trauma, disuse atrophy, jaw tumor surgery, congenital tooth loss, etc., there are a large number of cases where conventional implant restoration cannot be performed due to insufficient height or width of available bone in the implant area. . [0003] In the early 1980s, the scientific concept of guided tissue regeneration (Guided Tissue Regenerat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/58
CPCA61L27/3633A61L27/3687A61L27/50A61L27/58A61L2430/02A61L2430/40
Inventor 韩韦红葛翠兰钱锵张国强
Owner 江苏白衣缘生物工程有限公司
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