Medical decellularized biomaterial and preparation method thereof

A biomaterial and decellularization technology, applied in the field of medical decellularized biomaterials and their preparation, can solve the problems of easy rupture, tearing or other accidents, unfavorable recovery of new tissue, and rapid product degradation, and achieve good induction of tissue. Regeneration, good natural cross-linking degree, good toughness effect

Pending Publication Date: 2019-10-25
上海白衣缘生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] One of the purposes of the present invention is to solve the problem that some decellularized medical materials have poor stability, are easy to degrade, or degrade quickly in actual clinical use, and cannot effectively meet the actual needs of medical clinics. For example, the product degrades too quickly, which is not conducive to As a reliable and stable cell scaffold to assist the formation of new tissue at the site of injury and the recovery of function
[0011] The second object of the present invention is to solve the problem that some decellularized medical materials have poor and weak mechanical properties in actual clinical use, and are prone to rupture, tearing or other accidents in actual clinical use, which cannot effectively meet the needs of medical clinical practice. Need, for example, hernia mesh, tendon mesh, rotator cuff mesh and dura mater mesh with poor mechanical properties will appear bulging, tearing or cracking

Method used

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  • Medical decellularized biomaterial and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: (elimination of sow small intestine)

[0061] Select the preparation of decellularized medical materials derived from the small intestinal submucosa of healthy culled sows, and the specific steps are as follows:

[0062] 1) Pretreatment: In the slaughterhouse, the fresh small intestines of culled sows are selected, cleaned and used as the initial raw material for the patch; after in-depth research, the gestational age of culled binary mixed sows is usually above 6 parities, and the weight is 180 kg The above are all in the empty pregnant stage; the feeding time is usually more than 36 months; the mucosal layer, muscular layer, serosa layer, lymph nodes, etc. of the pig small intestine and jejunum are removed by physical scraping, the submucosa is separated, and soaked in 0.5% acetic acid solution for 30 Minutes, the ratio of pig small intestine to acetic acid solution was 1:5, and then rinsed with purified water 3 times to obtain the raw material of the biolo...

Embodiment 2

[0068] Example 2: (Commercial Pork Small Intestine Control Group 1)

[0069] The pretreatment of connective tissue, disinfection, degreasing, decellularization, DNA and α-Gal antigen removal, freeze-drying, sterilization and other steps are completely the same as in Example 1; the difference is only in the initial animal selection; selection of slaughter In the farm, the fresh small intestine tissue of the Du Chang Sanyuan miscellaneous commodity pork pig with a weight of about 100 kg was cleaned; it was learned that the number of days of breeding was about 160-180 days.

Embodiment 3

[0070] Example 3: (removal of sows --- leather)

[0071] The pretreatment of tissue raw materials, such as disinfection, degreasing, cell removal, DNA removal and α-Gal antigen removal, freeze-drying, and sterilization, are basically the same as in Example 1; but there are differences in the first step and the sixth step; the first step The difference is the different types of raw materials. In this example, skins from healthy culled sows (live pigs weighing more than 180 kg) from slaughterhouses were used as the initial raw materials. treatment, and remove the stratum corneum and epidermis, and use mechanical methods to make the thickness of the dermis 0.5mm, so as to facilitate subsequent treatments.

[0072] The second difference is that in the sixth step, the decellularized sheet material is divided into two pieces, instead of four layers like SIS; the overlapping is fixed on the mold, after freeze-drying, packaging, and finally sterilization.

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Abstract

The invention belongs to the field of medical repair materials, and relates to a medical decellularized material for tissue repair and a preparation method thereof. The medical decellularized materialand the preparation method thereof have the advantages that neither of a chemical cross-linking agent or an optical method and a physical method is applied to solve the problems of existing materials, including low stability, proneness to degradation and poor mechanical property, and accordingly, residues and toxicity of chemical reagents are avoided; connective tissue of breeding stocks, insteadof product fat stocks, is selected as a raw material, because the connective tissue of the breeding stocks has higher toughness and better biomechanical properties than that of the product fat stocks; the medical decellularized material can retain the advantages of existing decellularized materials, has higher stability and better mechanical properties, is not easy to break but easy to sew, has high airtightness, and is particularly suitable for tissue repair of tissue parts with high requirements on stability and mechanical properties.

Description

technical field [0001] The invention belongs to the field of medical biological materials, and relates to a medical decellularized biological material and a preparation method thereof. Background technique [0002] At present, biomaterials used in clinical medicine are divided into two categories: synthetic materials and natural biomaterials; synthetic materials have good mechanical strength, good stability, and are not easy to degrade, but they cannot induce tissue regeneration and healing. Natural biological materials are mostly decellularized materials, mainly derived from mammalian dermis, small intestine, pericardium and other tissues, which are processed by fat removal, cell removal, and DNA removal to obtain three-dimensional materials with extracellular matrix. The structure and some active ingredients, including collagen, non-collagen and growth factors, provide an adaptive environment for host cell adhesion, proliferation, and differentiation; contribute to the str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/58
CPCA61L27/362A61L27/3629A61L27/3687A61L27/3691A61L27/3695A61L27/50A61L27/58A61L2430/40
Inventor 韩韦红葛翠兰钱锵张国强
Owner 上海白衣缘生物工程有限公司
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