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Peach gum polysaccharide with efficient anti-allergy and itching-relieving effects, and fermentation extraction method and application thereof

A technology of peach gum polysaccharide and extraction method, applied in the field of bioengineering, can solve the problems of wide molecular weight distribution and poor homogeneity of the product, and achieve the effects of narrow molecular weight range, low emission, and improved anti-allergic and antipruritic effect.

Inactive Publication Date: 2020-06-05
MELLGEN SHENZHEN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the first object of the present invention is to provide a method for fermenting and extracting peach gum polysaccharides with high anti-allergic and antipruritic properties, so as to solve the problems of poor uniformity and wide molecular weight distribution of the existing peach gum polysaccharides.

Method used

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  • Peach gum polysaccharide with efficient anti-allergy and itching-relieving effects, and fermentation extraction method and application thereof
  • Peach gum polysaccharide with efficient anti-allergy and itching-relieving effects, and fermentation extraction method and application thereof
  • Peach gum polysaccharide with efficient anti-allergy and itching-relieving effects, and fermentation extraction method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1 Preparation of engineering yeast dry powder

[0035] 1) Preparation of yeast clones, extract the genomic DNA of the expression vector galactanase (sequence from NCBI: XM_025647395.1), digest with nuclease to obtain linearized DNA, and dissolve the linearized DNA in 5-10 μL TE (purchased from Neopro Biotech). Take 80 μL of commercially competent Pichia pastoris GS115 (purchased from Tiangen Biotechnology) and mix it with 10 μg of linearized DNA, and transfer it into a pre-cooled 0.2 cm electroporation cup. Place on ice for 5 min. Set the machine parameters, immediately add 1ml of pre-cooled 1M sorbitol to the cup, transfer the contents to a sterilized centrifuge tube and divide it into 200μL aliquots, spread it on the MD plate (basic glucose medium), and incubate the plate at 30°C Until the clone is produced, because the transformed vector contains the mut gene, only the successfully transformed strains can be screened by the mut phenotype, so the Mut+ / Mut...

Embodiment 2

[0037] Example 2 Extraction of Peach Gum Polysaccharide by High Pressure Water Extraction

[0038] After the peach gum is removed from debris, take about 100g of peach gum and add appropriate amount of water to soak overnight, add ultra-pure water according to the ratio of solid to liquid at 1:20, stir and extract with hot water at 80°C, the extraction time is 4 hours, and the number of times is 2 times. Due to the high viscosity of the solution, first filter it with 2 layers of gauze, then centrifuge the filtrate at 8000r / min for 15 minutes, pour out the supernatant, reduce the pressure and rotary evaporate to about 1 / 3 of the original volume, pour it out, and add absolute ethanol to The mass fraction of ethanol was 90%, and the supernatant was poured off after standing overnight. The lower precipitate was washed with absolute ethanol and vacuum filtered, then freeze-dried to obtain peach gum polysaccharide. The total sugar content of peach gum polysaccharide was determined ...

Embodiment 3

[0042] Example 3 Extraction of Fermented Peach Gum Polysaccharide

[0043] After the peach gum is removed from debris, take about 100g of peach gum and add appropriate amount of water to soak overnight, and add ultra-pure water according to the material-liquid ratio of peach gum, ultrapure water and yeast dry powder expressing galactanase 1:10:0.01 and yeast dry powder, and then poured into a 5L fermenter, adjusting the temperature to 30°C, rotating at 250rpm, stirring and fermenting for 10h. Measure the viscosity of the feed liquid, and stop the fermentation when the viscosity drops below 500mPa.s. Pour out feed liquid, centrifuge 10 minutes with high-speed centrifuge 6000rpm. Discard the bacterial sediment at the bottom, and use a roll-type filter membrane (pore size 0.22 microns) for microfiltration to remove residual bacterial cells and granular components without causing bacterial contamination. Add absolute ethanol until the mass fraction of ethanol is 70%, and pour of...

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Abstract

The invention discloses a fermentation extraction method of peach gum polysaccharide with efficient anti-allergy and itching-relieving effects. The method comprises the following steps of 1, mixing soaked peach gum, ultrapure water and yeast dry powder capable of expressing galactanase according to a proportion of 1:(5 to 100):(0.01 to 0.1), and performing stirring and fermentation; 2, when the viscosity of fermentation liquid is reduced to 200 to 1000 mPa.s, stopping stirring, pouring out the fermentation liquid, adding absolute ethyl alcohol until the mass percentage of the ethyl alcohol is60 to 90 percent, performing still standing, and removing supernatant; and 3, washing lower layer precipitates by the ethyl alcohol, and performing freeze drying after reduced pressure suction filtration to obtain the peach gum polysaccharide. The invention also discloses the peach gum polysaccharide with efficient anti-allergy and itching-relieving effects, application of the peach gum polysaccharide, and application of the peach gum polysaccharide to preparation of anti-allergy and itching-relieving skin care products. The fermentation extraction method of the peach gum polysaccharide has the advantages that the energy consumption is low, and the environment is protected. The peach gum polysaccharide obtained by using the method provided by the invention has the anti-allergy and itching-relieving effects.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a high-efficiency anti-allergy and antipruritic peach gum polysaccharide and its fermentation extraction method and application. Background technique [0002] Peach gum has a variety of medicinal values, and its main functions in medicine and beauty are lowering blood fat, lowering blood sugar, and moisturizing the skin. Peach gum has a large molecular weight and a highly cross-linked structure, making it difficult to dissolve. Soluble peach gum polysaccharides can usually be prepared by water swelling hydrolysis extraction, improved alcohol extraction, high pressure and other methods. Although the solubility of peach gum polysaccharides is improved and the molecular weight is greatly reduced, the uniformity of the product is poor. The molecular weight distribution is wide, and the skin barrier or intestinal mucosa system has a certain barrier and barrier effect on the ...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04C12N15/81C12N15/56A61K31/715A61P37/08A61P17/04A61K8/73A61Q19/00C12R1/84
CPCC12P19/14C12P19/04C12N15/815C12N9/2471C12Y302/01023A61K31/715A61P37/08A61P17/04A61K8/73A61Q19/00
Inventor 阮仁全
Owner MELLGEN SHENZHEN BIOTECHNOLOGY CO LTD
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