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High-yield paenibacillus polymyxa strain and application thereof

A technology of polymyxa spore and bacillus, which is applied in the field of high-yield Paenibacillus polymyxa and its application patent application field, can solve the problems of limited types, limited popularization and application, low fermentation yield and the like

Active Publication Date: 2020-05-19
河南新仰韶生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although Paenibacillus polymyxa is a good biocontrol and growth-promoting bacterium, it has a good market prospect and high application value, but it is limited by technical problems such as the low fermentation yield of Paenibacillus polymyxa. The types of microbial preparation products mainly based on Paenibacillus polymyxa are still relatively limited, which limits the promotion and application of such microbial preparation products in actual production, so it is extremely necessary to screen Paenibacillus polymyxa with better growth effects strain and further optimize the fermentation process of the strain, so as to lay a technical foundation for the production and application of related microbial preparations

Method used

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  • High-yield paenibacillus polymyxa strain and application thereof
  • High-yield paenibacillus polymyxa strain and application thereof
  • High-yield paenibacillus polymyxa strain and application thereof

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specific Embodiment

[0020] The present application will be further explained below in conjunction with the embodiments. Before introducing the specific embodiments, a brief description of some of the experimental backgrounds involved in the following embodiments is as follows.

[0021] Medium:

[0022] Nutrient Agar medium (Nutrient Agar.NA): peptone 10.00 g, beef extract 3.00 g, sodium chloride 5.00 g, agar 15.0 0 g, distilled water 1.00 L, pH 7.0;

[0023] Beef extract medium (Bee4.3 extract Agar): take 500.00 g of fat-free lean meat, chop it, add 1.00 L of distilled water to a beaker, put it in a refrigerator at 4 °C overnight, filter it with cotton, and boil the filtrate in a water bath 1 h, make the protein coagulate, filter, add 10.00 g peptone and 5.00 g sodium chloride to the filtrate, adjust the pH to 7.4, and make up the liquid to 1.00 L, 15.00 g agar, sterilize after aliquoting, and set aside;

[0024] Method for determining the viability of Paenibacillus polymyxa

[0025] Use the d...

Embodiment 1

[0032] In this example, the process of obtaining the Paenibacillus polymyxa YSD7 strain provided by mutagenesis and screening is briefly introduced as follows.

[0033] The Paenibacillus polymyxa YSD7 provided by this application is mainly obtained through ultraviolet mutagenesis and diethyl sulfate (DES) mutagenesis screening of the existing starting strain Paenibacillus polymyxa YSD5, and the specific mutagenesis and screening process are brief described as follows.

[0034] (1) Ultraviolet mutagenesis

[0035] The first is to carry out ultraviolet mutagenesis breeding on the starting strain YSD5, and the main process is as follows:

[0036] Take 4ml of YSD5 bacterial suspension cultured at 30°C to the growth stage of the opponent, and irradiate it with a 15 W ultraviolet lamp at a distance of 28 cm. During the irradiation process, the survival rate of the strain gradually decreases with the extension of the ultraviolet irradiation time. During the radiation mutagenesis pr...

Embodiment 2

[0055] On the basis of the strain obtained in Example 1, the inventors further optimized the fermentation process to further improve the growth and fermentation capacity of the strain. The specific process is introduced as follows.

[0056] (1) Strain activation and seed solution preparation

[0057] After the preserved Paenibacillus polymyxa YSD7 strain was activated and cultured in LB plate medium at 30°C for 2 days, the bacterial lawn was collected and transferred to LB liquid medium for further amplification, and then transferred to the seed medium of the seed tank medium, 30°C, 200rpm fermentation for about 18h as seed liquid;

[0058] The seed medium is: 2% glucose, 3% corn flour, 5% bean cake powder, 1% corn steep liquor powder, 0.2% potassium dihydrogen phosphate, 1.5% yeast powder, pH 7.0;

[0059] (2) Preparation of fermentation broth

[0060] According to the inoculation amount of 6% volume ratio, transfer the seed liquid prepared in step (1) to the fermentation ...

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Abstract

The invention belongs to the technical field of biological fermentation engineering, and specifically relates to a high-yield paenibacillus polymyxa strain and an application thereof. Paenibacillus polymyxa YSD7 is preserved at China General Microbiological Culture Collection Center on December 5, 2019, and a preservation number is CGMCC 19087. The paenibacillus polymyxa YSD7 can prevent and treaterwinia carotovora, taro soft rot bacteria, phytophthora nicotianae, flower root rot bacteria, bipolaris sorokiniana, cercospora personata, or polygonatum root rot bacteria. The inventors finally obtain the paenibacillus polymyxa strain YSD7 with relatively good growth vigor through the combination application of ultraviolet mutagenesis and diethyl sulfate mutagenesis technology, and qualitativescreening breeding based on the existing strain. Preliminary experimental results show that the highest fermentation activity of the YSD7 strain can reach 9.5 billion / mL, the YSD7 strain shows relatively good growth effects, and a good technical foundation for the preparation of paenibacillus polymyxa microbial preparation products can be laid.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering, and specifically relates to a high-yield Paenibacillus polymyxa and its application patent application. Background technique [0002] Paenibacillus polymyxa ( Paenibacillus polymyxa ), is a spore-forming, gram-positive rod-shaped bacterium with diverse physiological characteristics. When it exists in the form of spores, it has the advantages of good temperature resistance and stable quality. Paenibacillus polymyxa is also a common dominant bacterium between soil and plant microecology, so there are many researches and applications in the prevention and control of soil-borne diseases such as bacterial wilt, fusarium wilt, root rot, and soft rot. It has the characteristics of promoting growth, increasing yield, improving quality, and no residue. Therefore, microbial preparations based on Paenibacillus polymyxa are the preferred control agents for pollution-free, green, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N13/00C12N15/01A01N63/25A01P3/00C12R1/01
CPCC12N1/20C12N13/00C12N15/01A01N63/00C12R2001/01C12N1/205
Inventor 孙利鹏焦国宝管宁崔怡梁颖杰崔红建郭蒙恩朱飞帆
Owner 河南新仰韶生物科技有限公司
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