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Pseudomonas stutzeri and application thereof

A technology of Pseudomonas stutzeri and strains, which is applied in the fields of agricultural microorganism technology and environmental protection, can solve the problems such as difficulty in reducing nitrous oxide emission, and achieve the effects of reducing nitrous oxide emissions, having good application prospects and promoting growth.

Active Publication Date: 2020-05-08
NANJING UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Purpose of the invention: the technical problem to be solved by this invention is to provide a strain of Pseudomonas stutzeri and its application for the problem of difficulty in reducing the emission of greenhouse gas nitrous oxide in farmland soil in the prior art

Method used

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  • Pseudomonas stutzeri and application thereof
  • Pseudomonas stutzeri and application thereof
  • Pseudomonas stutzeri and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and screening of Pseudomonas stutzeri NRCB010

[0030] Weigh 10g of rice rhizosphere soil and transfer to the ultra-clean workbench for operation. Put the soil sample into an Erlenmeyer flask filled with 90mL of 0.87% NaCl solution, shake at 28°C and 200r / min for 1h, and prepare a dilution of 10 -1 soil suspension; take 10 -1 Put 10mL of the soil suspension into a conical flask filled with 90mL of 0.87% NaCl solution, mix well, and make a dilution of 10 -2 soil suspension; and so on. Diluted to a dilution of 10 -3 、10 -4 、10 -5 soil suspension. Then use a pipette to pipette 10 -3 、10 -4 、10 -5 100 μL of the diluted solution was spread on the nitrogen-fixing solid medium (each liter containing KH 2 PO 4 , 0.2g; K 2 HPO 4 , 0.8g; MgSO 4 ·7H 2 O, 0.2g; CaSO 4 2H 2 O, 0.1g; FeCl 3 , trace; Na 2 Mo 4 2H 2 O, trace; yeast extract, 0.5 g; mannitol, 20 g, agar, 15 g; pH 7.2, solvent was water), and each gradient was repeated in triplicat...

Embodiment 2

[0031] The identification of embodiment 2 Pseudomonas stutzeri NRCB010

[0032] When cultured on LB agar medium, the NRCB010 strain showed round and yellow colonies; the cells were short rod-shaped and 10 μm in length; see figure 1 . Gram stain was negative. Through 16S rDNA sequence analysis, it is homologous to Pseudomonas stutzeri after BLAST comparison in NCBI; the 16S rDNA sequence is shown in SEQ ID No.1. The physiological and biochemical characteristics are shown in Table 1 and figure 2 . Through whole genome sequencing, the genome circle map obtained is shown in image 3 .

[0033] Table 1 Physiological and biochemical properties of Pseudomonas stutzeri NRCB010

[0034]

[0035]

[0036] Remarks: "+" is exploitable or sensitive, "-" is not exploitable or insensitive, "\" is between "+" and "-".

Embodiment 3

[0037] Example 3 Under microcosmic conditions, Pseudomonas stutzeri NRCB010 reduces the emission analysis of dryland soil nitrous oxide

[0038] Soil for test: Collect the surface soil of vegetables in Yixing, Jiangsu, air-dry naturally, pass through a 2mm sieve, and set aside. Bacterial suspension preparation: pick a single colony of Pseudomonas stutzeri NRCB010 and inoculate it into NBNS (peptone 5gL -1 , beef dipping powder 3gL -1 , 0.3mM NaNO 3 and 4mM sodium succinate, pH 7.0, the solvent is water) culture medium, placed in a shaker at 28°C at 180rpm and cultured to the logarithmic growth phase, centrifuged at 4000 rpm for 15 minutes, and resuspended to OD with 1 / 100NBNS 600 = 1.0. Microcosm inoculation test: Weigh 100g of air-dried soil into a 500mL culture bottle, add 2mL of bacterial suspension, and mix thoroughly; add the same amount of 1 / 100NBNS to control 1; add the same amount of Escherichia coli DH5α (OD 600 = 1.0) of 1 / 100 NBNS; add an appropriate amount of s...

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Abstract

The invention discloses pseudomonas stutzeri and application thereof. The pseudomonas stutzeri disclosed by the invention is (Pseudomonas stutzeri) NRCB010, and the preservation number of the bacterium in the China General Microbiological Culture Collection Center is CGMCC NO.19067. Microverse experiments, greenhouse pot culture and field plot experiments show that the (Pseudomonas stutzeri) NRCB010 disclosed by the invention is capable of promoting crop growth and reducing emission of a soil greenhouse gas nitrous oxide.

Description

technical field [0001] The invention belongs to the field of agricultural microorganism technology and environmental protection, and relates to a strain of Pseudomonas stutzeri and its application. Background technique [0002] Nitrous oxide (N 2 O), is a greenhouse gas that can be transported to the stratosphere, causing the destruction of the ozone layer, causing an ozone hole, exposing humans and other organisms to solar ultraviolet radiation, and causing damage to human skin, eyes, and immune system. Compared with carbon dioxide, although the content of nitrous oxide in the atmosphere is very low, it is a trace gas, but it persists in the atmosphere for a long time, and the single-molecule warming potential is about 300 times that of carbon dioxide (IPCC, 2007). The warming effect of the increase of nitrous oxide concentration on the global climate has aroused great concern of scientists. [0003] Agricultural sources are an important anthropogenic source of nitrous ox...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/20C09K17/14C12R1/38C09K101/00
CPCC12N1/20C05F3/00C09K17/14C09K2101/00C12R2001/38C12N1/205Y02W30/40
Inventor 高南冷静申卫收牛欢青应汉杰徐虹张欢欢陆顺周超伟
Owner NANJING UNIV OF TECH
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