Yarrowia lipolytica capable of producing bisabolene and constructing method for yarrowia lipolytica and use of yarrowia lipolytica

Abstract

The invention discloses genetically engineered bacteria of yarrowia lipolytica capable of producing bisabolene and use of the genetically engineered bacteria, and belongs to the technical field of molecular biology. The genetically engineered bacteria are obtained by introducing an alpha-bisabolene synthase gene, a beta-bisabolene synthase gene or a gamma-bisabolene synthase gene into a host of the yarrowia lipolytica and by overexpression of a 3-hydroxy-3-methyl glutaryl coenzyme A reductase gene (HMGR gene). After the genetically engineered bacterium undergo shake-flask fermentation in respective yeast extract peptone dextrose (YPD) mediums, the yield of the bisabolene produced by genetically engineered bacteria Po1g Delta ku70 alpha-HR is 100.22 mg / L, the yield of the bisabolene produced by genetically engineered bacteria Po1g Delta ku70 beta-HR is 5.66 mg / L, and the yield of the bisabolene produced by genetically engineered bacteria Po1g Delta ku70 gamma-HR is 3.55 mg / L. After themediums are optimized, the yield of the bisabolene produced by the genetically engineered bacteria Po1g Delta ku70 alpha-HR is 162.24 mg / L, the yield of the bisabolene produced by genetically engineered bacteria Po1g Delta ku70 beta-HR is 20.811 mg / L, and the yield of the bisabolene produced by genetically engineered bacteria Po1g Delta ku70 gamma-HR is 6.25 mg / L.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a Yarrowia lipolytica genetic engineering bacterium producing bisabolene and its application. Background technique [0002] Yarrowia lipolytica is a typical representative unconventional yeast. The yeast is non-pathogenic, the maximum growth temperature is generally below 34°C, and has been identified as (generally regarded as safe, GRAS) safety-level microorganisms. Different from Saccharomyces cerevisiae, this yeast is strictly aerobic, and the maximum biomass value it can achieve is relatively large. Another remarkable advantage of Yarrowia lipolytica is that it widely exists in various foods and various living environments, because the yeast can use a wide range of carbon sources, including organic acids, alkanes, alkenes, oils, Alcohols, esters and other cheap carbon sources. In addition, Yarrowia lipolytica can produce and (or) secrete various metabolites during ...

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a strain introducing α-bisabolene synthase gene or β-bisabolene synthase gene or γ-bisabolene synthase gene or γ-bisabolene Yarrowia lipolytica genetically engineered bacteria with bisabolene synthase gene and the use of the strain in heterologous synthesis of bisabolene, which can synthesize bisabolene and construct bisabolene by means of metabolic engineering or synthetic biology. Engineering strains for alkene production (refer to attached figure 2 )

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