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Application of a kind of human cryptochrome protein i (hcry1) in the preparation of anti-ultraviolet radiation preparations

A pigment protein, ultraviolet radiation technology, applied in the application field of preparations, can solve the problems of toxic side effects, opacity, poor shading effect, etc.

Active Publication Date: 2020-12-18
GUANGZHOU DOUBLLE BIOPRODUCT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to develop safe, environmentally friendly, non-toxic sunscreen sunscreens, researchers have conducted a lot of research. However, the current sunscreens have the characteristics of toxic side effects and non-broad-spectrum absorption, poor shading effect and opacity. Therefore, it is urgent to develop a preparation process with simple, safe, light-transmitting, water-soluble and broad-spectrum absorption properties.

Method used

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  • Application of a kind of human cryptochrome protein i (hcry1) in the preparation of anti-ultraviolet radiation preparations
  • Application of a kind of human cryptochrome protein i (hcry1) in the preparation of anti-ultraviolet radiation preparations
  • Application of a kind of human cryptochrome protein i (hcry1) in the preparation of anti-ultraviolet radiation preparations

Examples

Experimental program
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Effect test

Embodiment 1

[0029]Example 1 Cell Experiment

[0030]Source of raw materials: UVA ultraviolet lamp (Guanya Optoelectronics, wave peak 360nm), UVB ultraviolet lamp (Guanya Optoelectronics, wave peak 320nm), UVC ultraviolet lamp (Beijing Stone, wave peak 257nm), UV illuminance meter (Taiwan Xianchi), enzyme label Instrument (Bio-Rad), fluorescent quantitative PCR instrument (Stratagene MX 3000P), hCRY1 recombinant human cryptochrome protein solution (Dubbio, E. coli expression and fermentation purification), CCK-8 kit (Shanghai Yisheng Biotechnology), 6 -8-week-old SD rats (Guangzhou Youdi Biotechnology), Anti-MMP-1 antibody (Bioss), SPF30 sunscreen (purchased from Meikaina), SYBR Green MIX (Shanghai Yisheng Biotechnology).

[0031]Preparation:

[0032](1) Cell culture: The HaCaT cells were resuscitated and cultured in DMEM medium containing 10% FBS, successively passaged, and the 3-10th passage cells were taken for experiment.

[0033](2) Preparation of UV irradiation box: Use a polystyrene foam box as the o...

Embodiment 2

[0062]Example 2 Animal experiment

[0063]Source of raw materials: Experimental animals: SD rats (purchased from Guangzhou Youdi Bio), 30, MMP-1 antibody (purchased from Bioss).

[0064]QPCR reagents (purchased from YEASEN): TRIZOL, reverse transcriptase, SYBR MIX, MMP-1, MMP-3, GAPDH real-time quantitative PCR primers.

[0065]Specimen preparation and immunohistochemistry: chloral hydrate, paraformaldehyde, paraffin wax, dewaxing agent, mounting tablets, slides, absolute ethanol, HE staining kit, immunohistochemistry secondary antibody kit, immunohistochemistry tool kit .

[0066]UVA UV lamp 15W (Guanya Optoelectronics, wave peak 360nm), UVB UV lamp 15W (Guanya Optoelectronics, wave peak 320nm), dimmer, UV light meter (Taiwan Xianchi), rat immobilizer, electric razor, QPCR instrument (Purchased from STRATAGENE).

[0067]A human cryptochrome protein I (hCRY1) emulsion. The emulsion includes the following raw materials: water, glycerin, polyethylene glycol-100 stearate, salicylate, sodium polyaceta...

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Abstract

The invention discloses an application of human cryptochrome protein I (hCRY1) in the preparation of an anti-ultraviolet radiation preparation, and belongs to the field of biopharmaceutical technology. This application carried out cell experiments and animal experiments respectively, through tissue morphology staining and immunohistochemical staining, and related gene mRNA expression levels to reveal and observe skin photoaging changes and gene expression differences in animal models, and then determine whether hCRY1 has UV protection function. hCRY1 is a potential new biological UV sunscreen in this study. Compared with physical sunscreens that are opaque, chemical sunscreens that have toxic side effects and non-broad-spectrum absorption, hCRY1 is safe, light-transmitting, water-soluble, and broad-spectrum. Features of absorbing 100‑400nm ultraviolet rays.

Description

Technical field[0001]The invention belongs to the field of biopharmaceutical technology, and specifically relates to the application of human cryptochrome protein I (hCRY1) in the preparation of anti-ultraviolet radiation preparations.Background technique[0002]Currently, sunscreen products on the market are mainly divided into physical sunscreens and chemical sunscreens. Physical sunscreens usually reflect or scatter ultraviolet and visible light. Commonly used substances include talc, zinc oxide, titanium dioxide, magnesium oxide, and kaolin. Chemical sunscreens usually absorb ultraviolet rays, and they have different effects depending on the wavelength of ultraviolet rays. For example, the sunscreens that absorb long-wave ultraviolet (wavelength range 320-400nm) include benzophenone, 4-tert-butyl-4-methoxymethane and 4-isopropyl diphenylmethane; absorb medium-wave ultraviolet (wavelength range) (290-320nm) sunscreens include p-aminobenzoic acid and its derivatives, cinnamate and s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K8/64A61P17/16A61Q17/04
CPCA61K8/64A61K38/1709A61Q17/04A61P17/16
Inventor 黄文林周晓鸿
Owner GUANGZHOU DOUBLLE BIOPRODUCT CO LTD
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